AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion

Huo, Yu Meng; Liu, Bing Jiang; Yang, Yan; Miao, Jun; Gao, Li; Kong, Su Ping; Wang, Zhen Bao; Kitano, Hidemi; Xiong, Wei

doi:10.1007/s10681-015-1374-7

Cited by 25 publications

(30 citation statements)

References 43 publications

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“…Huo et al. () also reported that jnurf13 and their newly developed AcSKP1 marker are considered as the same locus, because both originated from previous study of Yang et al. ().…”

Section: Discussionmentioning

confidence: 99%

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Khar

Saini

2016

Plant Breeding

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The main aim of this study was to validate PCR markers for determining cytoplasm and genotypes at the Ms locus in short-day onion. Three cytoplasmic (OSN, MKFR and accD) and four nuclear (OPT, jnurf13, AcSKP1 and AcPMS1) markers were employed. Sel. 121-1 had 100% S cytoplasm, whereas Sel. 121-2, 'Pusa Red' and 'Pusa Madhavi' had 88%, 33% and 17% S cytoplasm, respectively. 'Early Grano' and 'Pusa Riddhi' did not possess S cytoplasm. Observations in 33 commercial varieties revealed two with sterile (S) cytoplasm. Nuclear markers were not found in linkage disequilibrium with the Ms locus, and the constitution of Ms alleles by OPT was different from other three markers, which were in conformity with each other. The other three markers predicted that most of the plants should be homozygous recessive. Anther colour also did not confirm the sterility status. It can be concluded that accD may be used for cytoplasm determination based on the ease of its use. For the Ms locus tagging, more markers are needed to be evaluated to isolate maintainer lines from open-pollinated populations.

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“…Huo et al. () also reported that jnurf13 and their newly developed AcSKP1 marker are considered as the same locus, because both originated from previous study of Yang et al. ().…”

Section: Discussionmentioning

confidence: 99%

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Khar

Saini

2016

Plant Breeding

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“…Three SNP markers tightly linked with Ms loci were identified that remained in linkage disequilibrium in three OP populations (Havey 2013). Recently, Huo et al (2015) identified putative S-phase kinase-associated protein 1 (SKP1) protein in onion and developed PCR-based co-dominant markers from it, which are in linkage disequilibrium with Ms loci. Bulb onion DNA mismatch repair (AcPMS1) gene found to be most probable candidate for regulating fertility restoration.…”

Section: Hybrid Productionmentioning

confidence: 99%

Enhancing onion breeding using molecular tools

et al. 2015

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Bulb onion (Allium cepa L.) is an ancient crop that is thought to have originated in Central Asia and has been cultivated for over 5000 years. Classical genetic and plant breeding approaches have been used to improve onion yield, quality, and resistance against biotic and abiotic stresses. However, its biennial life cycle, cross-pollinated nature and high inbreeding depression have proved challenging for the characterization and breeding of improved traits. New technologies, notably next-generation sequencing, are providing researchers with the genomic resources and approaches to overcome these challenges. Using these genomic technologies, molecular markers are being rapidly developed and utilized for germplasm analysis and mapping in onion. These new tools and knowledge are allowing the integration of molecular and conventional breeding to speed up onion improvement programmes. In this review, we outline recent progress in onion genomics and molecular genetics and prospects for enhancing onion yield and quality in the future.

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“…, Havey , Huo et al. , Kim et al. ), but their validation is needed before applying them in MAS of maintainer lines (Saini et al.…”

Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion

Cited by 25 publications

References 43 publications

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Enhancing onion breeding using molecular tools

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

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