Acrylic scaffolds with interconnected spherical pores and controlled hydrophilicity for tissue engineering

Diego, Raúl Brígido; Olmedilla, M. P.; Aroca, Ángel; Ribelles, J.L. Gómez; Pradas, Manuel Monleón; Ferrer, Gloria Gallego; Salmerón-Sánchez, Manuel

doi:10.1007/s10853-005-3885-4

Cited by 38 publications

(21 citation statements)

References 37 publications

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“…It is worth noting that pores collapse is highly anisotropic. As shown in Figure 1 and table 1, the diameter of the pores observed are similar to that of the original scaffold, while in average in the dimension perpendicular to the surface they had to be reduced by the ratio R. On the other hand, pore morphology depends on cross-linking density [21]. By increasing crosslinking density swelling capacity of the polymer network decreases [47,48] and, as a consequence, volumetric changes in the scaffold trabeculae during swelling and deswelling are smaller.…”

supporting

confidence: 52%

“…Macroporous scaffolds were prepared with a template technique [20,21,41]. Template was formed by sintering poly (methyl methacrylate) PMMA (Lucite International Colacryl DP 300, USA) microspheres, with diameter ranging from 90 to 120 µm.…”

Section: Macroporous Membranes Preparationmentioning

confidence: 99%

“…If the scaffold cannot provide a mechanical modulus in the range of native tissues, then any nascent tissue formation will probably fail [18]. Conventional techniques to produce scaffolds for tissue engineering applications such as template or particle leaching techniques [19][20][21][22][23][24][25] with pore sizes in the order of several tens of microns would yield scaffolds that would disaggregate when cut to produce membranes with thicknesses in the order of magnitude of pore sizes. Freeze extraction or freeze gelation techniques [24,[26][27][28][29][30][31][32] yield materials with very high porosity what makes them very weak in the form of thin films.…”

Section: Introductionmentioning

confidence: 99%

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Macroporous thin membranes for cell transplant in regenerative medicine

et al. 2015

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ElsevierAntolinos Turpín, CM.; Morales Román, RM.; Ródenas Rochina, J.; Gómez Ribelles, JL.; Gómez-Tejedor, JA. (2015). Macroporous thin membranes for cell transplant in regenerative medicine. Biomaterials. 67:254-263. doi:10.1016/j.biomaterials.2015.07.032. Biomaterials 67 (2015 ABSTRACTThe aim of this paper is to present a method to produce macroporous thin membranes made of poly (ethyl acrylate-co-hydroxyethyl acrylate) copolymer network with varying cross-linking density for cell transplantation and prosthesis fabrication. The manufacture process is based on template techniques and anisotropic pore collapse. Pore collapse was produced by swelling the membrane in acetone and subsequently drying and changing the solvent by water to produce 100 microns thick porous membranes. These very thin membranes are porous enough to hold cells to be transplanted to the organism or to be colonized by ingrowth from neighboring tissues in the organism, and they present sufficient tearing stress to be sutured with surgical thread. The obtained pore morphology was observed by Scanning Electron Microscope, and confocal laser microscopy. Mechanical properties were characterized by stress-strain experiments in tension and tearing strength measurements. Morphology and mechanical properties were related to the different initial thickness of the scaffold and the cross-linking density of the polymer network.Seeding efficiency and proliferation of mesenchymal stem cells inside the pore structure were determined at 2 hours, 1, 7, 14 and 21 days from seeding.

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supporting

confidence: 52%

Section: Macroporous Membranes Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Macroporous thin membranes for cell transplant in regenerative medicine

et al. 2015

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“…Scaffolds were prepared using a template made of PMMA microspheres with 90 µm average diameter (Colacryl DP 300, Lucite International) that were sintered under pressure at temperatures above its glass transition temperature, according to the procedure previously described (36). The microspheres were introduced into a mould consisting of two glass plates separated by a rubber ring.…”

Section: Polymeric Scaffoldsmentioning

confidence: 99%

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

Sancho‐Tello

Forriol²,

Gastaldi³

et al. 2015

Int J Artif Organs

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The Authors state that this manuscript has not been published previously and is not currently being assessed for publication by any journal other than The InternationalJournal of Artificial Organs.Each Author has contributed substantially to the research, preparation and production of the paper and approves of its submission to the Journal. FINANCIAL SUPPORT CONFLICT OF INTERESTThe authors state they do not have proprietary interest. MEETING PRESENTATIONThis study has been partially presented at the following meeting: ABSTRACT Purpose: Tissue engineering techniques were used to study cartilage repair over 12-month period in a rabbit model. Methods:A full-depth chondral defect along with subchondral bone injury were originated in the knee joint, where a biostable porous scaffold was implanted, synthesized of poly(ethyl acrylate-co-hydroxyethyl acrylate) copolymer. Morphological evolution of cartilage repair was studied 1 and 2 weeks, and 1, 3 and 12 months after implantation by histological techniques. Three-month group was chosen to compare cartilage repair to an additional group where scaffolds were preseeded with allogeneic chondrocytes before implantation, and also to controls, who underwent the same surgery procedure, with no scaffold implantation.

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“…Las características de la estructura molecular del PEA, movilidad de la cadena lateral, la polaridad y su baja hidrofilicidad, permiten que tenga lugar una interacción directa entre el polímero y las proteínas de la matriz extracelular. Por otra parte, es posible llevar a cabo la preparación de materiales ultraporosos, que permiten la invasión celular y que se utilizan como guías para el crecimiento celular, son los "scaffolds" (21)(22)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36) . Además mediante distintas variantes de la técnica de porógeno-lixiviación se consiguen distintas estructuras porosas (27-29, 31, 33-36) .…”

Section: Estrategias Actuales De Regeneración Neural: Uso De "Scaffolunclassified

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Gamboa-MartÍnez

Ribelles

Ferrer

2011

Journal of Bioactive and Compatible Polymers

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A hybrid scaffold was obtained by the deposition of a thin network of submicron fibrin fibrils on the microporous walls of a macroporous poly(L-lactide) (PLLA) three-dimensional structure. The fibrin coating is homogeneous across the entire substrate and allowed the pore structure remain open in the hybrid scaffold. The elastic modulus of the hybrid scaffold (0.65 MPa) was increased up to twofold compared to the pure PLLA scaffold (0.29 MPa). Mouse pre-osteoblastic cells, MC3T3, were seeded on both pure PLLA and hybrid scaffolds, and cultured for 3, 6, and 24 h. The coating enhanced the cell colonization and proliferation and provided a more homogeneous distribution of cells within the scaffolds. In addition, the coating improved the scaffold adhesion properties by supplying new binding sites to the cells that modify the transmembrane receptors involved in initial cell adhesion mechanism. The expression of the β3 integrin was observed in cells cultured on fibrin-coated scaffolds instead of the α5 integrin, which was expressed in the uncoated scaffold. These hybrid PLLA/fibrin scaffolds have cell culture features suitable to promote early tissue regeneration.

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Acrylic scaffolds with interconnected spherical pores and controlled hydrophilicity for tissue engineering

Cited by 38 publications

References 37 publications

Macroporous thin membranes for cell transplant in regenerative medicine

Macroporous thin membranes for cell transplant in regenerative medicine

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

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