Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Vale, Fernando Martins do; Castro, Matilde; Monteiro, Jacinto; Couto, Frederico S.; Pinto, Rui; Rico, J.M. Gia ̃o Toscano

doi:10.1016/s0142-9612(97)00043-4

Cited by 58 publications

(39 citation statements)

References 4 publications

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“…MMA is a toxic, highly volatile organic solvent. Cytotoxic effects on human fibroblasts in culture media have been described and attributed to MMA (Vale et al 1997). Exposure to MMA vapors may have irritating effects on mucous membranes of the respiratory tract and the eyes (Fregert 1983, Nissen andCorydon 1985).…”

mentioning

confidence: 99%

Efficacy of vacuum bone cement mixing systems in reducing methylmethacrylate fume exposureComparison of 7 different mixing devices and handmixing

Schlegel

Sturm

Ewerbeck

et al. 2004

Acta Orthopaedica Scandinavica

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Background Polymethylmethacrylate (PMMA) bone cements are mainly used for implant fixation in joint replacement surgery. During cement preparation for application, all staff in the operating theatre are exposed to methylmethacrylate (MMA) fumes, which are known to have toxic side effects.Methods and results In this study we found that vacuum mixing of bone cement with 7 commercially available mixing devices significantly reduced the emission of MMA vapors in the breathing zone when compared with classic hand mixing in an open bowl. Gas chromatography appears to be more sensitive for detection of MMA fumes than Photo Inonization Detection.Interpretation According to present knowledge, even repeated mixing of PMMA bone cement during a normal working day does not seem to constitute an increased health risk, particularly if vacuum mixing is implemented.

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mentioning

confidence: 99%

Efficacy of vacuum bone cement mixing systems in reducing methylmethacrylate fume exposureComparison of 7 different mixing devices and handmixing

Schlegel

Sturm

Ewerbeck

et al. 2004

Acta Orthopaedica Scandinavica

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“…However, free radicals are, among other side products, potentially cytotoxic for osteoblastic cells, as previously reported by Moreau et al [16]. Furthermore, Vale et al [17] demonstrated a significant increase in lipoperoxide liberation from fibroblasts and a reduced cell viability upon stimulation with medium which had previously been exposed to PMMA. These findings clearly indicate a limited biocompatibility of these compounds, possibly accounting for late implant loosening phenomena.…”

Section: Introductionmentioning

confidence: 70%

“…Consequently, the cytotoxicity should be limited to the duration of the polymerization. In contradiction to this hypothesis, Vale et al [17] demonstrated a significantly reduced cell viability of human fibroblasts upon stimulation with medium which had previously been exposed to polymerized PMMA. Confirming their results, we also identified a significant reduction in cell viability and DNA content on Palacos R and Palacos R+G surfaces after 24 h. After 72 h, a considerably higher cell viability and DNA content was observed for all surfaces.…”

Section: Discussionmentioning

confidence: 98%

In vitro Growth Pattern of Primary Human Osteoblasts on Calcium Phosphate- and Polymethylmethacrylate-Based Bone Cement

Schauwecker

Bock²,

Pohlig³

et al. 2017

Eur Surg Res

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Background/Purpose: Polymethylmethacrylate (PMMA) and calcium phosphate (Ca-P) cements are widely used for arthroplasty surgery and augmentation of bone defects. However, aseptic implant loosening in absence of wear-induced osteolysis indicates an unfavourable interaction between the cement surface and human osteoblasts. Our underlying hypothesis is that cement surfaces directly modify cell viability, proliferation rate, and cell differentiation. Methods: To test this hypothesis, we examined primary human osteoblasts harvested from six individuals. These cells were pooled and subsequently seeded directly on cement pellets prepared from Palacos® R, Palacos® R+G, and Norian® Drillable cements. After incubation for 24 and 72 h, cell viability, proliferation rate, apoptosis rate, and cell differentiation were analysed. Results: Upon cultivation of human osteoblasts on cement surfaces, we observed a significantly reduced cell viability and DNA content compared to the control. Analysis of the apoptosis rate revealed an increase for cells on Palacos R and Norian Drillable, but a significant decrease on Palacos R+G compared to the control. Regarding osteogenic differentiation, significantly lower values of alkaline phosphatase enzyme activity were identified for all cement surfaces after 24 and 72 h compared to cultivation on tissue culture plastic, serving as control. Conclusions: In summary, these data suggest a limited biocompatibility of both PMMA and Ca-P cements, necessitating further research to reduce unfavourable cell-cement interactions and consequently extend implant survival.

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“…The acrylic resin consisting of the lowest level of monomer releases the least amount of methyl methacrylate monomer. But resins with more resident monomer may release no more methyl methacrylate (22). 59.3% of participants knew about this issue.…”

Section: Discussionmentioning

confidence: 99%

Undergraduate Prosthodontics Students’ Knowledge of Working With Methyl Methacrylate at Shahid Sadoughi Medical University in 2014

Lotfikamran¹,

Gholamhosseini²,

Tafti³

2016

Avicenna J Dent Res

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Background: The toxicity of the methyl methacrylate monomer is an ongoing concern in dentistry. Therefore, those who are involved with this substance need to be cognizant of how to work with it. Objectives: This study aimed to evaluate the level of working knowledge about methyl methacrylate among undergraduate prosthodontics students at Shahid Sadoughi university in 2014. Materials and Methods: In this descriptive cross-sectional study, the knowledge level of 54 undergraduate prosthodontics students, who had passed the last 2 years of their education, was assessed via a 13-question survey related to methyl methacrylate. Each correct answer was assigned 2 points, and the total sum of right answers was used to measure the knowledge level of each participant. In order to analyze the study data, SPSS16 was used to apply an ANOVA test, t-test, and Pearson correlation coefficient.

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Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Cited by 58 publications

References 4 publications

Efficacy of vacuum bone cement mixing systems in reducing methylmethacrylate fume exposureComparison of 7 different mixing devices and handmixing

Efficacy of vacuum bone cement mixing systems in reducing methylmethacrylate fume exposureComparison of 7 different mixing devices and handmixing

In vitro Growth Pattern of Primary Human Osteoblasts on Calcium Phosphate- and Polymethylmethacrylate-Based Bone Cement

Undergraduate Prosthodontics Students’ Knowledge of Working With Methyl Methacrylate at Shahid Sadoughi Medical University in 2014

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