Acrostichus rhynchophori n. sp. (Rhabditida: Diplogastridae): a phoretic associate of Rhynchophorus cruentatus Fabricius and R. palmarum L. (Coleoptera: Curculionidae) in the Americas

Herrmann

2013

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Micoletzkya masseyi n. sp. and M. japónica n. sp. from bark beetles Orthotomicus caelatus and Dryocoetes uniseriatus, respectively, are described based on morphology and molecular data. Both species are gonochoristic and can be cultured monoxenically on baeteria. Micoletzkya masseyi n. sp. is differentiated from other species of the genus by a very short male tail, P6-P8 genital papillae arranged in a triangle and with P7 being eloser to P8 than to P6, and a eonoid female tail to a sharp terminus. Micoletzkya japónica n. sp. is distinct from other Micoletzkya speeies by the arrangement of its male papillae such that P6 and P7 are close to each other and P8 is positioned apart from them, a conoid male tail with a spicate terminus, and a small gubernaculum {ca 11 /xm in length). Phylogeny ofthe genus was inferred using nucleotide sequences of 18S rRNA, 28S rRNA, COI, and two ribosomal protein genes. Biologieal data for the new species are presented and the associations between Micoletzkya species and bark beetles are discussed.

Section: Discussionmentioning

confidence: 99%

Description of the bark beetle associated nematodes Micoletzkya masseyi n. sp. and M. japonica n. sp. (Nematoda: Diplogastridae)

Susoy

Herrmann

2013

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“…The arrangement of genital papillae were labelled as 'GP + number', e.g., GP1 and GP2 as in Kanzaki et al (2009).…”

Section: Methodsmentioning

confidence: 99%

“…is similar to A. puri and A. halicti. These three species share several characteristic morphological traits, viz., modification of anterior end of cheilostomatal plate, duplicated gymnostomatal wall, very complicated gubernaculum structure, distally hooked spicule with flap-like rostrum (Giblin & Kaya, 1984;Kanzaki et al, 2009;Kanzaki et al, unpubl.). Furthermore, all species share phoretic associations with halictid bees, albeit different species that nest in different substrates (soil for H. ligatus and rotting wood for A. pura and Megalopta spp.).…”

Section: Diagnosis and Relationshipmentioning

confidence: 99%

Acrostichus megaloptae n. sp. (Nematoda: Diplogastridae), a phoretic associate of Megalopta spp. (Hymenoptera: Halictidae) in Central America

Giblin-Davis

Wcislo

et al. 2010

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Adults of the dusk-flying and rotting wood-nesting bees Megalopta genalis and/or M. ecuadoria were collected at Barro Colorado Island, The Smithsonian Tropical Research Institute (BCI, STRI), Panama and La Selva, Costa Rica, and examined for nematodes. Nematodes were recovered as dauer juveniles from the Dufour's gland of female bees and aedeagus of male bees. Adult nematodes isolated from M. genalis (BCI and La Selva) and M. ecuadoria (BCI) were successfully cultured and kept in the laboratory. All three populations were used for morphological observations and molecular analyses and were identified as a new species that is described herein as Acrostichus megaloptae n. sp. It is characterised by its stomatal morphology, possession of six triangular cuticle flaps covering stomatal opening, duplicated cheilo-and gymnostomatal walls, large dorsal tooth and sclerotised ventral stegostomatal ridges, female with vulval flap, male spicule and gubernaculum morphology, i.e., relatively straight spicule with oval-shaped manubrium, rounded flap-like rostrum and separated and strongly ventrally curved spicule with bifurcate tip, gubernaculum L-shaped in lateral view and anchor-shaped in ventral view, and filiform tail of both sexes. Sequences from the three A. megaloptae n. sp. geographical/host isolates were not significantly different and molecular phylogenetic analysis and biological and morphological comparisons place the new species close to A. halicti from Halictus ligatus and A. puri from Augochlora pura from North America, although the new species is distinguished from A. halicti and A. puri based upon its stomatal morphology of males and females, male spicule and gubernaculum morphology and female vulval structure.

“…These papillae were confirmed in all of the more than 50 specimens examined, confirming the stability of this character in R. humicolus n. sp. Fe male vulval papillae have not been reported in any other diplogastrid species examined by scanning electron mi croscopy (SEM), e.g., Acrostichus Rahm, 1928(Kanzaki et al, 2009), Diplogastrellus Paramonov, 1952(Kanzaki e ta i, 2008, Micoletzkya Weingartner, 1955(Susoy et al, 2013, Parasitodiplogaster Poinar, 1979(Giblin-Davis et al, 2006, or Pristionchus (Photos e ta i, 2006). However, these small papillae are not conspicuous, and could easily be overlooked during light microscopic observation.…”

Section: Description Of Cryptic Speciesmentioning

confidence: 99%

Rhabditidoides humicolus n. sp. associated with arthropods from rotting palm tissue in Florida, USA

Giblin-Davis

2015

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Rhabditidoides humicolus n. sp. is described and illustrated from arthropods associated with decaying tissue from the crown shaft of a living spindle palm, Hyophorbe verschajfeltii, in southern Florida, USA. In addition to its generic character, i.e., the arrangement of male genital papillae, (vl, v2, v3d, CO, v4, (ad, v5, ph, v6), (pd, v7)), the new species is characterised by its small stomatal flaps, a secretory pore-like opening, a pair of deirids, two pairs of post-deirids and small subventral vulval papillae located just anterior to the vulva. Besides those newly found characters, there are only a few typological differences between R. humicolus n. sp. and several previously described species in the genus. However, based on the biological characters, e.g., gonochoristic reproduction, association as dauers with the crane fly, Limonia (Rhipidia) schwarzi (Diptera: Limoniidae), millipedes, an immature cockroach, and staphylinid beetle adults, and distribution in southern Florida, the new species was considered to be different from others in the genus.