Across the Gobi Desert: impact of landscape features on the biogeography and phylogeographically-structured release calls of the Mongolian Toad, Strauchbufo raddei in East Asia

Othman, Siti N.; Choe, Minjee; Chuang, Ming‐Feng; Purevdorj, Zoljargal; Maslova, Irina V.; Schepina, Natalya; Jang, Yikweon; Borzée, Amaël

doi:10.1007/s10682-022-10206-4

Cited by 3 publications

(3 citation statements)

References 98 publications

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“…Genomic DNA was extracted from ethanol preserved liver or tail tissue samples using the HiPure Universal DNA Kit (Magen Biotech, Guangzhou, China). To incorporate the data published by Zhao et al [32], we amplified the mitochondrial DNA cytochrome As the range of E. argus and the Mongolian toad (Strauchbufo raddei) overlap in most of the modern areas, we categorized all sampling sites a priori into seven geographic regions (Northern region: Mongolia and Russia; Southwestern region: Qinghai Province; Northeast region: Liaoning and Heilongjiang provinces; Central region: Inner Mongolia; Southern region: the Ningxia, Shanxi, Shaanxi, Hebei, Gansu provinces and Beijing Municipality; Southeastern region: Shandong, Anhui and Henan provinces; Eastern Region: Korean Peninsula) and five landscapes (canyon, plateau, river basin, steppe, and lowland forest) [33,34].…”

Section: Dna Extraction Amplification and Sequence Analysismentioning

confidence: 99%

“…We used the similar division of landscapes (Steppe: Inner Mongolia, Beijing, Shanxi, Hebei, Mongolia and Russia; River: Heilongjiang and Liaoning provinces; Canyon: Shaanxi and Gansu provinces; Lowland Forest: Shandong, Henan and Anhui provinces and Korea; Plateau: Gonghe County, Qinghai Province.) in Othman et al [33]. Variation was calculated between groups, between populations within groups and within populations.…”

Section: Population Genetic Analysismentioning

confidence: 99%

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Range-Wide Phylogeography and Ecological Niche Modeling Provide Insights into the Evolutionary History of the Mongolian Racerunner (Eremias argus) in Northeast Asia

Tian,

Xu,

Chen

et al. 2024

Animals

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The Mongolian racerunner, Eremias argus, is a small lizard endemic to Northeast Asia that can serve as an excellent model for investigating how geography and past climate change have jointly influenced the evolution of biodiversity in this region. To elucidate the processes underlying its diversification and demography, we reconstructed the range-wide phylogeographic pattern and evolutionary trajectory, using phylogenetic, population genetic, landscape genetic, Bayesian phylogeographic reconstruction and ecological niche modeling approaches. Phylogenetic analyses of the mtDNA cyt b gene revealed eight lineages that were unbounded by geographic region. The genetic structure of E. argus was mainly determined by geographic distance. Divergence dating indicated that E. argus and E. brenchleyi diverged during the Mid-Pliocene Warm Period. E. argus was estimated to have coalesced at~0.4351 Ma (Marine Isotope Stage 19). Bayesian phylogeographic diffusion analysis revealed out-of-Inner Mongolia and rapid colonization events from the end of the Last Interglacial to the Last Glacial Maximum, which is consistent with the expanded suitable range of the Last Glacial Maximum. Pre-Last Glacial Maximum growth of population is presented for most lineages of E. argus. The Glacial Maximum contraction model and the previous multiple glacial refugia hypotheses are rejected. This may be due to an increase in the amount of climatically favorable habitats in Northeast Asia. Furthermore, E. argus barbouri most likely represents an invalid taxon. The present study is the first to report a range-wide phylogeography of reptiles over such a large region in Northeast Asia. Our results make a significant contribution towards understanding the biogeography of the entire Northeast Asia.

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Section: Dna Extraction Amplification and Sequence Analysismentioning

confidence: 99%

Section: Population Genetic Analysismentioning

confidence: 99%

Range-Wide Phylogeography and Ecological Niche Modeling Provide Insights into the Evolutionary History of the Mongolian Racerunner (Eremias argus) in Northeast Asia

Tian,

Xu,

Chen

et al. 2024

Animals

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“…Furthermore, this method can yield less DNA than tissues [ 34 ], and a reptile study found the type of swab and DNA extraction method used can affect buccal swabbing success [ 35 ]. Despite these concerns, buccal swabbing has recently been used for collecting phylogeographic [ 43 , 44 ], and population and conservation genetic data [ 45–47 ].…”

Section: Introductionmentioning

confidence: 99%

Comparing skin swabs, buccal swabs, and toe clips for amphibian genetic sampling, a case study with a small anuran (Acris blanchardi)

Rainey,

Tryc,

Nicholson

2024

Biology Methods and Protocols

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Multiple methods for collecting genetic samples from amphibians exist, each with their own implications for study design, animal welfare, and costs. Toe clipping is one common method, but there is ongoing debate regarding its potential detriment. Less invasive methods should be implemented, if efficacious, as amphibians are a particularly vulnerable vertebrate group. Skin and buccal swabbing are less invasive methods for genetic sampling, but the potential for contamination and a lower yield of DNA may exist. To compare these methods, we gathered skin swabs, buccal swabs, and toe clips from the same individuals of a relatively small anuran species, Blanchard’s Cricket Frog (Acris blanchardi). We then compared DNA yield, DNA purity, amplification success rate, and genotypic data quality among sample types. We found toe clips and buccal swabs generated similar DNA yield and purity, with skin swabs yielding significantly less DNA of significantly lower purity than the other sample types. Amplification success rate was significantly higher using toe clips compared to the other sample types, though buccal swab samples amplified more readily than skin swabs. Genotypic data from toe clips and buccal swabs did not differ significantly in quality, but skin swab data quality was significantly lowest among sample types. Thus, skin swabbing could produce erroneous data in some situations, but buccal swabbing is likely an effective substitute to toe clipping, even for small species. Our results can help future researchers select which genetic sampling method might best suit their research needs.

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Bufonidae (Bufo & Strauchbufo)

Borzée

2024

Continental Northeast Asian Amphibians

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Across the Gobi Desert: impact of landscape features on the biogeography and phylogeographically-structured release calls of the Mongolian Toad, Strauchbufo raddei in East Asia

Cited by 3 publications

References 98 publications

Range-Wide Phylogeography and Ecological Niche Modeling Provide Insights into the Evolutionary History of the Mongolian Racerunner (Eremias argus) in Northeast Asia

Range-Wide Phylogeography and Ecological Niche Modeling Provide Insights into the Evolutionary History of the Mongolian Racerunner (Eremias argus) in Northeast Asia

Comparing skin swabs, buccal swabs, and toe clips for amphibian genetic sampling, a case study with a small anuran (Acris blanchardi)

Bufonidae (Bufo & Strauchbufo)

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