Acrosome reaction of human sperm used for in vitro fertilization

Plachot, M.; Mandelbaum, J.; Junca, A. M.

doi:10.1016/s0015-0282(16)48083-0

Cited by 56 publications

(29 citation statements)

References 18 publications

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“…3A). These data are consistent with previous observations showing a low incidence (2-7%) of spontaneous acrosome reactions (evaluated by triple stain, chlortetracyline or immunofluorescence) in human sperm capacitated for 5-8 hr in vitro (Plachot et al, 1984;Boyers et al, 1987;Cross et al, 1988;Yudin et al, 1988). However, we observed changes in the incidence of sperm with completely intact acrosomes and altered acrosomal caps during culture for 0-6 hr.…”

Section: Acrosomal Statussupporting

confidence: 93%

Evidence that anandamide‐signaling regulates human sperm functions required for fertilization

Schuel

Burkman

Lippes

et al. 2002

Molecular Reproduction Devel

128

123

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Ejaculated mammalian sperm require several hours exposure to secretions in female reproductive tracts, or incubation in appropriate culture medium in vitro, before acquiring the capacity to fertilize eggs. Arachidonylethanolamide (AEA), also known as anandamide, is a novel lipid-signal molecule that is an endogenous agonist (endocannabinoid) for cannabinoid receptors. We now report that AEA is present in human seminal plasma, mid-cycle oviductal fluid, and follicular fluid analyzed by high-performance liquid chromatography/mass spectrometry. Sperm are sequentially exposed to these reproductive fluids as they move from the vagina to the site of fertilization in the oviduct. Specific binding of the potent cannabinoid agonist [3 H]CP-55,940 to human sperm was saturable (K D 9.71 AE 1.04 nM), suggesting that they express cannabinoid receptors. R-methanandamide , a potent and metabolically stable AEA analog, and (À)D 9 tetrahydrocannabinol (THC), the major psychoactive constituent of Cannabis, modulated capacitation and fertilizing potential of human sperm in vitro. AM-356 elicited biphasic effects on the incidence of hyperactivated sperm motility (HA) between 1 and 6 hr of incubation: at (2.5 nM) it inhibited HA, while at (0.25 nM) it stimulated HA. Both AM-356 and THC inhibited morphological alterations over acrosomal caps between 2 and 6 hr (IC 50 5.9 AE 0.6 pM and 3.5 AE 1.5 nM, respectively). Sperm fertilizing capacity, measured in the Hemizona Assay, was reduced 50% by (1 nM) AM-356. These findings suggest that AEA-signaling may regulate sperm functions required for fertilization in human reproductive tracts, and imply that smoking of marijuana could impact these processes. This study has potential medical and public policy ramifications because of the incidence of marijuana abuse by adults in our society, previously documented reproductive effects of marijuana, and the ongoing debate about medicinal use of marijuana and cannabinoids.

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Section: Acrosomal Statussupporting

confidence: 93%

Evidence that anandamide‐signaling regulates human sperm functions required for fertilization

Schuel

Burkman

Lippes

et al. 2002

Molecular Reproduction Devel

128

123

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“…Results reported in this paper support previous findings where, under normal in vitro conditions, without the use of ionophores and in isosmotic media, the percentage of reacting cells is relatively low [17,19,221. This reflects the heterogeneity in the maturation state of spermatozoa present in an ejaculate and in their in vitro ability to undergo metabolic changes associated with capacitation and acrosome reaction.…”

Section: Discussionsupporting

confidence: 91%

“…The use of fluorescein-conjugated Ricinus communis lectin does not differentiate between normally reacted and degenerated acrosomes of dead spermatozoa. Moreover, the interspecies model differs from the human in vitro fertilization, where no correlation was established between the rate of acrosome reaction and that of fertilization [17]. In the latter case spermatozoa have to make their way across the follicular cell mass and the zona pellucida before contacting the oolemma.…”

Section: Discussionmentioning

confidence: 96%

Acrosome-Reacting Capacity of Frozen-Thawed Human Semen: Relation to Hamster Ova Penetration

Pilikian

Guérin

1986

Archives of Andrology

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Interspecies egg penetration requires completion of capacitation and acrosome reaction of the spermatozoa. The ability of frozen-thawed human semen to accomplish this reaction was studied, and its relation to the zona-free hamster egg penetration was investigated. The percentage of reacted cells in the population of live spermatozoa estimated by the triple-staining technique was found to be greater in frozen than in fresh samples. However, the egg-penetrating ability of cryopreserved semen remained significantly low. Individual differences in the ability to undergo the acrosome reaction and its relation to penetration are discussed.

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“…I1 est bien admis maintenant que le pourcentage de spermatozoides humains qui accomplit spontan6ment la RA dans les milieux classiques de FIV demeure faible (3 fi 12%) quels que soient le milieu et le temps d'incubation [50,51,52,53]. De plus, la r6action spontan6e donne peu d'indication sur l'aptitude des spermatozoides/~ r6agir au contact de la membrane pellucide, h~ducteur physiologique de la r6action du spermatozoide qui va p6n6trer l'ovocyte.…”

Section: Interet Et Conditions D'etudeunclassified

“…Certains auteurs n'ont pas retrouv6 une v6ritable corr61ation entre le taux de clivage et celui de la RA [51]. D'autres, au contraire, ont relevd qu'un pourcentage anormalement dlevd de spermatozoa'des rdagis avant tout traitement dtait souvent lid ~ une fdcondance diminude [56,60].…”

Section: Relation Entre La Ra Et La F6condan-ce Du Spermatozoideunclassified

La réaction acrosomique

Pilikian

1994

Androl.

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RESUME La c a p a c i t~ d e s s p e r m a t o z o i d e s a c c o m p l i r la r~a c t i o n a c r o s o m i I N T R O D U C T I O NLa fonction principale du spermatozo~de est d'apporter son ADN ~ l'ovocyte Pour cela il se sert ~ la fois de sa mobilit5 et de ses enzymes localis~es dans la partie la plus ant~rieure de sa t~te : L'ACROSOME.L'acrosome est une v~sicule aplatie entour~e d'une m e m b r a n e off l'on distingue la m e m b r a n e acrosomique interne face ~ la membrane nucl~aire et la membrane acrosomique externe face ~ la membrane plasmique du spermatozoade. Les membranes acrosomiques interne et externe se joignent au niveau du segment ~quatoriaL. L'acrosome peut ~tre assimil~ ~ une v~sicule s~cr~-toire ; les enzymes h y d r o l y t i q u e s qu'elle contient (acrosine, hyaluronidase, neuraminidase pour ne citer que les principales) sont lib6r6es par un ph~nom~ne d'exocytose suite ~ un signal appropri~ et participent v r a i s e m b l a b l e m e n t h la p r o g r e s s i o n des gametes males ~ travers les multiples enveloppes ovocytaires. L'activation ct la liberation s~lectives de ces enzymes ~ mesure que les spermatozoades approchent l'ovocyte, sont contr61~es par les ph~nom~nes complexes de capacitation et de r~action acrosomique, difficilement dissociables.La n~cessit~ pour les spermatozoa'des ~jacu-l~s de subir des modifications pour acqu~rir la capacit~ de f~conder les ovocytes a ~t~ d~crite pour la premiere fois en 1951 [1,2]. Depuis, malgr~ le nombre considerable de t r a v a u x il s u b s i s t e encore u n e c e r t a i n e confusion sur le sujet. Cela tient d'une part, aux conditions dans lesquelles ces ph~no-37

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Acrosome reaction of human sperm used for in vitro fertilization

Cited by 56 publications

References 18 publications

Evidence that anandamide‐signaling regulates human sperm functions required for fertilization

Evidence that anandamide‐signaling regulates human sperm functions required for fertilization

Acrosome-Reacting Capacity of Frozen-Thawed Human Semen: Relation to Hamster Ova Penetration

La réaction acrosomique

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