Acrosome reaction and chromatin integrity as additional parameters of semen analysis to predict fertilization and blastocyst rates

Tello-Mora, Pamela; Hernández‐Cadena, Leticia; Pedraza, J.; López-Bayghen, Esther; Quintanilla‐Vega, Betzabet

doi:10.1186/s12958-018-0408-0

Cited by 25 publications

(16 citation statements)

References 73 publications

(75 reference statements)

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“…The capability to undergo the acrosome reaction is associated with fertility status and ART outcome. [50][51][52] Our results demonstrated the involvement of CD147 in the acrosome reaction. Therefore, we reasoned that the level of CD147 may be a marker to predict the fertilization rate and ART outcome.…”

Section: The Seminal Cd147 Level Is Associated With Ivf Outcomessupporting

confidence: 61%

CD147 deficiency is associated with impaired sperm motility/acrosome reaction and offers a therapeutic target for asthenozoospermia

Chen

Shi

et al. 2021

Molecular Therapy - Nucleic Acids

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Patients with asthenozoospermia often present multiple defects in sperm functions apart from a decrease in sperm motility. However, the etiological factors underlying these multifaceted defects remain mostly unexplored, which may lead to unnecessary treatment and unsatisfactory assisted reproductive technologies (ART) outcome. Here, we show that the protein levels of CD147 were lowered in sperm obtained from asthenozoospermic infertile patients exhibiting defects in both sperm motility and the acrosome reaction. Whereas CD147 maintained sperm motility before capacitation, female tract-derived soluble CD147 interacted with sperm-bound CD147 to induce an acrosome reaction in capacitated sperm. Soluble CD147 treatment restored the acrosome reaction and improved the fertility of sperm from patients with asthenozoospermia. Mechanistically, CD147 promotes sperm motility and acrosome reaction (AR) by eliciting Ca 2+ influx through soluble CD147 binding to sperm-bound CD147. Notably, the level of soluble CD147 in seminal plasma was positively correlated with the fertilization rate and pregnancy outcome in infertile couples undergoing in vitro fertilization. Our study has identified a marker for the diagnosis and a therapeutic target for the defective AR capability in asthenozoospermia and a candidate for the prediction of in vitro fertilization outcomes for male infertile patients that facilitates the development of precision medicine in ART.

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Section: The Seminal Cd147 Level Is Associated With Ivf Outcomessupporting

confidence: 61%

CD147 deficiency is associated with impaired sperm motility/acrosome reaction and offers a therapeutic target for asthenozoospermia

Chen

Shi

et al. 2021

Molecular Therapy - Nucleic Acids

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“…Therefore, supplementation of antioxidants could ameliorate sperm oxidative stress and improve sperm quality measures during cold storage [ 12 ]. In confirmation, we showed that melatonin supplementation could protect canine sperm acrosome and DNA integrity which are important determinants for the sperm’s future fertility [ 13 ]. As we observed a simultaneous decline in the Day 5 MDA levels in the treatment groups, the protective effects of melatonin might have come from its antioxidant properties.…”

Section: Discussionmentioning

confidence: 96%

Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

et al. 2022

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Background Sperm freezing and cold storage are the two most common assisted reproductive technologies in the canine breeding industry. The freeze-thawing process causes significant detrimental changes in both sperm cell structure and function. Previous research has confirmed that excessive accumulation of un-scavenged free radicals (oxidative stress) plays an important role in the cryopreservation-induced damage to sperm cells. Also, the gradual accumulation of the free radicals during cold storage leads to a decline in the sperm quality markers. Melatonin is an endogenous neurohormone synthesized from tryptophan amino acid by pineal glands. Besides its several well-known physiologic roles, melatonin has a significant antioxidant potential through direct free radical scavenging properties. Therefore, the current study was designed to evaluate the potential in vitro protective properties of melatonin (0.5, 1, and 2 mM) on canine sperm cells after freezing or during long-term cold storage (9 days, 5 °C) on most important sperm in vitro fertility markers. Results Melatonin at 0.5, 1- or 2-mM concentrations could preserve significantly higher sperm total motility after 4 days of cold storage. However, only the 1- and 2 mM melatonin concentrations could result in better TM and PM values after 7 days of cold storage. Furthermore, melatonin supplementation could preserve higher sperm viability and acrosome integrity after 7 days of storage. Also, it could have significant protective effects on the cooled sperm DNA integrity. In the freezing section of the current research, melatonin at either 1- or 2-mM concentrations could not improve the sperm post-thaw TM and PM, whereas they improved sperm DNA integrity. Also, the post-thaw plasma membrane functional integrity and sperm velocity parameters were not affected by the treatment. Although DMSO (Dimethyl Sulfoxide) as the melatonin solvent could reduce the level of sperm lipid peroxidation and even improve the post-thaw sperm DNA integrity compared to the negative control, it reduced the post-thaw sperm progressive motility. However, the negative effects were reversed by concurrent melatonin supplementation at 1- and 2-mM concentrations. Conclusion The addition of 1- or 2-mM melatonin to the canine sperm freezing and cooling media could improve sperm motility, viability, acrosome, and DNA integrity.

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“…In fact, it was reported that the use of spermatozoa with damaged DNA did not affect the results of fertilization and early embryonic development until blastocyst formation. 33 , 34 …”

Section: Discussionmentioning

confidence: 99%

Predictive Significance of Sperm DNA Fragmentation Testing in Early Pregnancy Loss in Infertile Couples Undergoing Intracytoplasmic Sperm Injection

Lê

Nguyen

et al. 2021

RRU

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Objective This study aimed to determine the role of sperm DNA fragmentation as a marker that could predict early pregnancy loss (EPL), either independently or in combination with another marker or markers, after intracytoplasmic sperm injection (ICSI) cycles. Methods This prospective descriptive cohort study retrieved data from 162 couples who underwent their first ICSI cycles at the Center for Reproductive Endocrinology and Infertility of Hue University Hospital in Vietnam from May 2018 to December 2019. General characteristics, semen parameters, sperm DNA fragmentation index (DFI), fertilization, embryo development, pregnancy rates, and EPL were assessed. The receiver-operating characteristic (ROC) method was performed to identify the threshold of DFI in EPL. Multivariate analysis was used to demonstrate the relationship between the sperm DNA fragmentation level and EPL. Results Of 162 ICSI cycles, 23 (14.2%) involved EPL. There was no significant difference between the sperm DNA fragmentation rate and the overall rate of pregnancy loss, although the negative pregnancy outcome group had a median DFI that was higher than that of the positive pregnancy outcome group (20% vs 17.8%). The ROC analysis indicated that a sperm DNA fragmentation rate of 16.6% was the priority cut-off that could be used to distinguish EPL with a sensitivity of 73.9% (95% confidence interval [CI], 67.15–80.67) and specificity of 47.48% (95% CI, 39.79–55.17). The multivariate analysis confirmed that in female factors such as age, body mass index (BMI), and sperm DNA fragmentation level affected the EPL rate. However, a combination of the sperm DNA fragmentation level and female age or female BMI could not sufficiently predict EPL. Conclusion EPL can be affected by multiple factors including sperm DNA fragmentation; however, there is no sufficient evidence indicating that sperm DNA fragmentation, both as a single marker and combined with other markers, is a good predictor of EPL.

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Acrosome reaction and chromatin integrity as additional parameters of semen analysis to predict fertilization and blastocyst rates

Cited by 25 publications

References 73 publications

CD147 deficiency is associated with impaired sperm motility/acrosome reaction and offers a therapeutic target for asthenozoospermia

CD147 deficiency is associated with impaired sperm motility/acrosome reaction and offers a therapeutic target for asthenozoospermia

Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

Predictive Significance of Sperm DNA Fragmentation Testing in Early Pregnancy Loss in Infertile Couples Undergoing Intracytoplasmic Sperm Injection

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