Acrolein Production by Bacteria Found in Distillery Grain Mashes

Studies on the Formation of Acrolein in Distillery Mashes1

“…The organism which has been isolated and studied in this investigation is distinctly different from those previously reported to produce acrolein. It also is distinct morphologically from those organisms studied by Serjak, Day, Van Lanen, and Boruff (1953). Further investigation will be required to determine whether the bacterium reported here and those reported by the above investigators are related or whether they belong to different genera.…”

Metabolism of Glycerol by an Acrolein-Forming Lacto-Bacillus

“…The organism used was lactobacillus strain 208-A, a representative of group H described by 261 on September 21, 2020 by guest http://jb.asm.org/ Downloaded from Serjak et al (1954). The stock culture was maintained in tomato juice agar (Garey et al, 1945).…”

“…Little work has been directed toward the mechanism of acrolein formation although the dehydration of glycerol in aqueous media poses intriguing biochemical questions. Both Mills et al (1954) and Serjak et al (1954) speculated that glycerol was attacked by acroleinproducing lactobacilli only after fermentable carbohydrate was largely utilized, i.e., that sugar has a sparing action on glycerol. This view was supported by the observations that acrolein is not formed in the presence of high levels of glucose and that glycerol is not actively fermented as a sole substrate.…”

“…This study is concerned with the identification of the products of glycerol dissimilation by an organism found by Serjak et al (1954) to produce acrolein. A metabolic pathway of glycerol metabolism is proposed.…”

Production of 3-Hydroxypropionaldehyde from Glycerol

“…1) via Lactobacillus sp. strain 208-A (12), an organism identified as a contaminant of grain alcohol fermentations (7,10). When resting cells were allowed to metabolize [1-14C]glycerol, they produced equimolar concentrations of labeled 1,3propanediol and 3-hydroxypropionic acid.…”