Acrolein Determination by Means of Tryptophane. A Colorimetric Micromethod

Abstract

A new colorimetric method of analysis for acrolein has the advantages over previous methods of a spectrally purer color with e maximum in the absorption curve, a colorless blank, and greater specificity. The method is based on the color produced by the condensation of acrolein with tryptophane Induced by concentrated hydrochloric acid, and is capable of detecting quantitatively as little as 15 micrograms of acrolein. The method is suitable for quantitative work only in the absence of other aldehydes, unless their concentration is relatively much less than that of the acrolein.

Production of 3-Hydroxypropionaldehyde in Silage Inoculated withLactobacillus coryniformisPlus Glycerol

“…Since several kinds of other aldehydes are also known to be alkali-unstable colorable components, 15) data differentiating between 3-HPA and other colorable aldehydes is also required to confirm 3-HPA production in complex matrices. For this purpose, we examined the absorption spectrum of HLC.…”

“…2) has been reported to be characteristic only of 3-HPA and acrolein. 15,19) It appears unlikely that HLC was acrolein, since it has been shown that acrolein is not produced in a microbiological glycerol fermentation process without thermal distillation of 3-HPA. 19) In addition, when LAB lacked 3-HPA productivity, or when glycerol, substrate for 3-HPA, was not added, HLC was not produced in the silage (Tables 1 and 3).…”

“…The colorimetric treatment is based on the color reaction between tryptophan and certain colorable components, e.g., nitrite and several aldehydes, in the presence of a concentrated acid, such as HCl. 15) The first modification of the colorimetric treatment was replacement of HCl with H 3 PO 4 . The second modification was replacement of acrolein as standard with a 3-HPA-containing solution that was quantitatively analyzed by high performance liquid chromatography (HPLC).…”

“…The absorption spectrum was compared with those of the 3-HPA standard and a control silage sample to confirm that the HLC was 3-HPA. Determination of the absorption spectrum was based on a report by Circle et al, 15) which indicates that the absorption spectrum after colorimetric treatment is dependent on aldehyde. This experiment was conducted as an alternative to thin-layer chromatography analysis, which proved to be difficult due to degradation of HLC in our attempt to lyophilize the silage extract as a pretreatment.…”

Semicarbazide-functionalized resin as a new scavenger for in situ recovery of 3-hydroxypropionaldehyde during biotransformation of glycerol by Lactobacillus reuteri

“…The colorimetric method of Circle et al (1945) as modified by Ulmer and Zeng (2007) was used for measuring total 3HPA (reuterin) concentration using acrolein as standard.…”