Acrolein, an α,β-Unsaturated Carbonyl, Inhibits Both Growth and PSII Activity in the Cyanobacterium<i>Synechocystis</i>sp. PCC 6803

Shimakawa, Ginga; Iwamoto, Tatsuya; Mabuchi, Tomohito; Saito, Ryota; Yamamoto, Hiroshi; Amako, Katsumi; Sugimoto, Tsuneaki; Makino, Amane; Miyake, Chikahiro

doi:10.1271/bbb.130186

Cited by 19 publications

(14 citation statements)

References 53 publications

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“…). These data suggest that acrolein did not accumulate in ΔMDR cells, at least not over 30 μ m , under the growth condition used in this study, even though it is detected in S. 6803 WT cells . Acrolein is known to be generated by lipid peroxidation of trienoic fatty acids .…”

Section: Discussionmentioning

confidence: 58%

“…We evaluated the effects of acrolein on the relative chlorophyll fluorescence during steady‐state photosynthesis in S. 6803 WT, ΔMDR, and ΔAKR. Previously, we found that the addition of acrolein to cells during steady‐state photosynthesis leads to a decrease in PSII activity, and a rise in the relative level of chlorophyll fluorescence . In the current study, we added acrolein at 100 μ m to S. 6803 WT, ΔMDR, and ΔAKR.…”

Section: Resultsmentioning

confidence: 75%

“…Asterisks indicate statistically significant differences between WT and DMDR (P < 0.05). is detected in S. 6803 WT cells [16]. Acrolein is known to be generated by lipid peroxidation of trienoic fatty acids [2,9].…”

Section: Discussionmentioning

confidence: 99%

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Medium‐chain dehydrogenase/reductase and aldo‐keto reductase scavenge reactive carbonyls in Synechocystis sp. PCC 6803

2018

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Reactive carbonyls (RCs), which are inevitably produced during respiratory and photosynthetic metabolism, have the potential to cause oxidative damage to photosynthetic organisms. Previously, we proposed a scavenging model for RCs in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803). In the current study, we constructed mutants deficient in the enzymes medium-chain dehydrogenase/reductase (ΔMDR) and aldo-keto reductase (ΔAKR) to investigate their contributions to RC scavenging in vivo. We found that treatment with the lipid-derived RC acrolein causes growth inhibition and promotes greater protein carbonylation in ΔMDR, compared with the wild-type and ΔAKR. In both ΔMDR and ΔAKR, photosynthesis is severely inhibited in the presence of acrolein. These results suggest that these enzymes function as part of the scavenging systems for RCs in S. 6803 in vivo.

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Section: Discussionmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 75%

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Medium‐chain dehydrogenase/reductase and aldo‐keto reductase scavenge reactive carbonyls in Synechocystis sp. PCC 6803

2018

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“…On the other hand, lipid-derived reactive carbonyls, including acrolein, 4-hydroxy-2-nonenal, and malondialdehyde, are produced via lipid peroxidation by ROS [ 23 ]. These carbonyls react with amino acid residues, such as lysine and cysteine, to inactivate protein function [ 24 , 25 ]. The amount of carbonylated proteins is an indicator of oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

A Carbon Dioxide Limitation-Inducible Protein, ColA, Supports the Growth of Synechococcus sp. PCC 7002

2017

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A limitation in carbon dioxide (CO 2 ), which occurs as a result of natural environmental variation, suppresses photosynthesis and has the potential to cause photo-oxidative damage to photosynthetic cells. Oxygenic phototrophs have strategies to alleviate photo-oxidative damage to allow life in present atmospheric CO 2 conditions. However, the mechanisms for CO 2 limitation acclimation are diverse among the various oxygenic phototrophs, and many mechanisms remain to be discovered. In this study, we found that the gene encoding a CO 2 limitation-inducible protein, ColA, is required for the cyanobacterium Synechococcus sp. PCC 7002 (S. 7002) to acclimate to limited CO 2 conditions. An S. 7002 mutant deficient in ColA (∆colA) showed lower chlorophyll content, based on the amount of nitrogen, than that in S. 7002 wild-type (WT) under ambient air but not high CO 2 conditions. Both thermoluminescence and protein carbonylation detected in the ambient air grown cells indicated that the lack of ColA promotes oxidative stress in S. 7002. Alterations in the photosynthetic O 2 evolution rate and relative electron transport rate in the short-term response, within an hour, to CO 2 limitation were the same between the WT and ∆colA. Conversely, these photosynthetic parameters were mostly lower in the long-term response of a few days in ∆colA than in the WT. These data suggest that ColA is required to sustain photosynthetic activity for living under ambient air in S. 7002. The unique phylogeny of ColA revealed diverse strategies to acclimate to CO 2 limitation among cyanobacteria.

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“…In chloroplasts, ROS can also be generated due to the excitation energy in photosystems I and II, which can then impair the growth of oxygenic phototrophs [32,33]. Further, ROS can react with membrane lipids and free fatty acids to produce α,β-unsaturated carbonyls such as acrolein [34], which can also inhibit growth of oxygenic phototrophs [35]. In general, oxygenic phototrophs developed defence mechanisms against the effects of ROS and dicarbonyl stress.…”

Section: Discussionmentioning

confidence: 99%

Responses of the chloroplast glyoxalase system to high CO2 concentrations

Shimakawa

Ifuku

Suzuki

et al. 2018

Bioscience, Biotechnology, and Biochemistry

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Sugar metabolism pathways such as photosynthesis produce dicarbonyls, e.g. methylglyoxal (MG), which can cause cellular damage. The glyoxalase (GLX) system comprises two enzymes GLX1 and GLX2, and detoxifies MG; however, this system is poorly understood in the chloroplast, compared with the cytosol. In the present study, we determined GLX1 and GLX2 activities in spinach chloroplasts, which constituted 40% and 10%, respectively, of the total leaf glyoxalase activity. In Arabidopsis thaliana, five GFP-fusion GLXs were present in the chloroplasts. Under high CO concentrations, where increased photosynthesis promotes the MG production, GLX1 and GLX2 activities in A. thaliana increased and the expression of AtGLX1-2 and AtGLX2-5 was enhanced. On the basis of these findings and the phylogeny of GLX in oxygenic phototrophs, we propose that the GLX system scavenges MG produced in chloroplasts during photosynthesis.

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Acrolein, an α,β-Unsaturated Carbonyl, Inhibits Both Growth and PSII Activity in the CyanobacteriumSynechocystissp. PCC 6803

Cited by 19 publications

References 53 publications

Medium‐chain dehydrogenase/reductase and aldo‐keto reductase scavenge reactive carbonyls in Synechocystis sp. PCC 6803

Medium‐chain dehydrogenase/reductase and aldo‐keto reductase scavenge reactive carbonyls in Synechocystis sp. PCC 6803

A Carbon Dioxide Limitation-Inducible Protein, ColA, Supports the Growth of Synechococcus sp. PCC 7002

Responses of the chloroplast glyoxalase system to high CO2 concentrations

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