Acridine Orange Staining of Decapod Crustacean Cuticle

Marlowe, R. L.; Dillaman, Richard M.

doi:10.2307/3226956

Cited by 12 publications

(11 citation statements)

References 12 publications

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“…Epifluorescence of stained surfaces was observed under ultraviolet light using a Zeiss Standard microscope equipped with Neofluor objectives, a Zeiss mercury lamp, excitation filter (365 nm), and transmission cut-off filter (400 nm). Acridine orange is used to stain for sulphated sugars (Dauphin, 2003;Marlowe and Dillman, 1995).…”

Section: Acridine Orange Stainingmentioning

confidence: 99%

In situ distribution and characterization of the organic content of the oyster shell Crassostrea gigas (Mollusca, Bivalvia)

Dauphin

Ball

Castillo‐Michel

et al. 2013

Micron

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Cultivation of commercial oysters is now facing the possible influence of global change in sea water composition, commonly referred to as "ocean acidification". In order to test the potential consequence of the predicted environmental changes, a cultivation experiment was carried out. The left and right valves of the oyster shell Crassostrea gigas differ in their structure; moreover, lenses of non compact layers are irregular. The shell layers of juvenile C. gigas are studied using a variety of highly spatially resolved techniques to establish their composition and structure. Our results confirm the presence of three different calcitic structural types. The role of the lenses of chalky layers is not yet deciplered. Despite a common mineralogy, the elemental composition of the layers differs. The sulphur aminoacids and sulphated polysaccharide contents of the intracrystalline and intercrystalline matrices differ, as well as those of the structural types. The possible different sensitivity of these structures to environmental changes is still unknown.

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Section: Acridine Orange Stainingmentioning

confidence: 99%

In situ distribution and characterization of the organic content of the oyster shell Crassostrea gigas (Mollusca, Bivalvia)

Dauphin

Ball

Castillo‐Michel

et al. 2013

Micron

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“…1C, arrows). Various chromatic results are obtained, ranging from green (acridine orange emitting as a monomer) to orange/red Xuorescence (acridine orange emitting as a polymer) (Marlowe and Dilliman, 1995).…”

Section: Acridine Orange Staining Of Early Mineralization Zonesmentioning

confidence: 99%

The two-step mode of growth in the scleractinian coral skeletons from the micrometre to the overall scale

Cuif¹,

Dauphin²

2005

Journal of Structural Biology

146

114

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“…4A,B, 5A-C, 6B,E). The particular morphology of crustacean cuticle, with multilaminar appearance in some sections and a swirling pattern of chitin fibrils in others, is unmistakable (Johnson, 1980;Stevenson, 1985;Felgenhauer, 1991;Marlowe and Dillaman, 1995). The crustacean cuticle is primarily composed of the multilaminate, chitinous procuticle (exo-and endocuticle), but may also be calcified by the deposition of mineral crystals (usually calcium salts).…”

Section: Cellular Composition and Functionmentioning

confidence: 99%

Studies of male sexual tubes in hermit crabs (crustacea, decapoda, anomura, paguroidea). I. Morphology of the sexual tube in Micropagurus acantholepis (Stimpson, 1858), with comments on function and evolution

Tudge

Lemaitre

2003

Journal of Morphology

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The external morphology and internal structure of the male sexual tube of the hermit crab Micropagurus acantholepis, a member of the family Paguridae from Australian waters, is described in detail using histological thick sectioning and scanning and transmission electron microscopy techniques. This is the first in-depth study of a sexual tube in the Paguroidea, a group where a remarkable number of genera (55.9% in the family Paguridae) with species having these intriguing sexual structures are known. In M. acantholepis a sexual tube is present on the left side, whereas only a gonopore is present on the right side. The tube is used for the delivery of spermatophores to the female and consists of a sheath of cuticular origin surrounding an internal, functional extension of the posterior vas deferens. Pedunculate spermatophores were observed within the lumen and partially extruding from the terminal opening of the tube in preserved specimens. The tube protrudes from the left coxa of the fifth pereopod as an elongate 3-mm-long, hollow, coiled structure with a terminal opening. Exteriorly the tube consists of a conspicuous thick chitinous cuticular ridge throughout its length, and a thin chitinous cuticle with sparse, regularly arranged simple setae. Interior to the cuticle, the tube contains loose connective tissue, secretory cells, oblique muscle, circular muscle, and epithelial cells. The latter cells line a central lumen that runs the length of the sexual tube. The morphology, cellular composition, and function of the tube are discussed.

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Acridine Orange Staining of Decapod Crustacean Cuticle

Cited by 12 publications

References 12 publications

In situ distribution and characterization of the organic content of the oyster shell Crassostrea gigas (Mollusca, Bivalvia)

In situ distribution and characterization of the organic content of the oyster shell Crassostrea gigas (Mollusca, Bivalvia)

The two-step mode of growth in the scleractinian coral skeletons from the micrometre to the overall scale

Studies of male sexual tubes in hermit crabs (crustacea, decapoda, anomura, paguroidea). I. Morphology of the sexual tube in Micropagurus acantholepis (Stimpson, 1858), with comments on function and evolution

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