Acridine — ihre physikochemische und biochemische Bedeutung Eine Betrachtung anläßlich der Entdeckung des Acridins vor 100 Jahren: Teil II

Löber, G.

doi:10.1002/zfch.19710110404

Zeitschrift fuer Chemie

1971

DOI: 10.1002/zfch.19710110404

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Acridine — ihre physikochemische und biochemische Bedeutung Eine Betrachtung anläßlich der Entdeckung des Acridins vor 100 Jahren: Teil II

G. Löber¹

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1976

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Cited by 16 publications

References 140 publications

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Effect of organic solvents on the properties of the complexes of DNA with proflavine and similar compounds

Löber¹,

Schütz²,

Kleinwächter

1972

Biopolymers

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SynopsisIn order to obtain information on the binding forces involved in the formation of the complex proflavine-DNA by the stronger process I, the stability of the complexes was investigated in the presence of various organic solvents, methanol, ethanol, n-propanol, isopropanol, formamide, dimethyl sulfoxide, p-dioxane, glycerol, and ethylene glycol. Quantitative data on binding in terms of K l n and r were obtained by means of absorption and fluorescence spectra, as well as by a thermal denaturation technique.The effectiveness of the solvents increases with their hydrocarbon content, but can hardly be related to their dielectric constant. The complex formation is effectively suppressed by organic solvent concentrations, in which DNA still preserves its double-helical conformation. These results demonstrate the importance of hydrophobic forces in the formation of the complex proflavine-DNA in aqueous solution.The similarity in spectroscopic properties of proflavine bound to DNA by process I and the same dye dissolved in an organic solvent make it possible to interpret the observed red shift of the long-wavelength absorption peak as being due to the interaction of the dye molecules with the less polar environment.The same behavior was found for other dyes capable of intercalation like purified trypaflavine, phenosafranine and ethidium bromide. However, intercalation is not a necessary condition, as it was shown in the case of pinacyanol, which binds only a t the surface of DNA.All organic solvents used decrease the binding ability of the dye.

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Effect of organic solvents on the properties of the complexes of DNA with proflavine and similar compounds

Löber¹,

Schütz²,

Kleinwächter

1972

Biopolymers

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Geometry of DNA–dye intercalation complexes from the study of linear dichroic spectra of stretched films

Kelly

Kurucsev

1976

Biopolymers

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SynopsisFilms of DNA-dye complexes were combined with films of pure DNA deposited on poly-(vinyl alcohol) support and stretched. Reproducible dichroic spectra were obtained after equilibration of the stretched films at 93% relative humidity. Dye diffusion into the supporting poly(viny1 alcohol) matrix was eliminated. The long axis of intercalated acriflavine is perpendicular to the DNA helix axis; proflavine deviates slightly and 9-aminoacridine significantly from such an intercalation geometry. The dichroism of two mutually perpendicularly polarized transitions of 9-aminoacridine enabled us to determine both the angles of tilt and twist of the plane of the dye relative to the DNA helix in the complex.

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ChemInform Abstract: ACRIDINE, IHRE PHYSIKOCHEMISCHE UND BIOCHEMISCHE BEDEUTUNG, BETRACHTUNG ANLAESSLICH DER ENTDECKUNG DES ACRIDINS VOR 100 JAHREN 2. MITT.

LOEBER¹

1971

Chemischer Informationsdienst. Organische Chemie

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Acridine — ihre physikochemische und biochemische Bedeutung Eine Betrachtung anläßlich der Entdeckung des Acridins vor 100 Jahren: Teil II

Cited by 16 publications

References 140 publications

Effect of organic solvents on the properties of the complexes of DNA with proflavine and similar compounds

Effect of organic solvents on the properties of the complexes of DNA with proflavine and similar compounds

Geometry of DNA–dye intercalation complexes from the study of linear dichroic spectra of stretched films

ChemInform Abstract: ACRIDINE, IHRE PHYSIKOCHEMISCHE UND BIOCHEMISCHE BEDEUTUNG, BETRACHTUNG ANLAESSLICH DER ENTDECKUNG DES ACRIDINS VOR 100 JAHREN 2. MITT.

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