Acquisition of haemoglobin-bound iron by Histophilus somni

Tremblay, Yannick D. N.; Bahrami, Fatemeh; Niven, Donald F.

doi:10.1016/j.vetmic.2005.11.053

Cited by 5 publications

(5 citation statements)

References 132 publications

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“…In particular, H. somnus strains that contain single-component transferrin receptors encoded by the tbpA2 gene can acquire iron from bovine, ovine, and goat transferrins (16), but those that possess bipartite transferrin receptors encoded by the tbpA and tbpB genes can acquire iron only from bovine transferrin (16,74). Phase variation of hemoglobin and transferrin receptor genes has been demonstrated in virulent H. somnus strains 2336 and 649 (16,18,67). Taken together with our finding that commensal strain 129Pt contains truncated hemoglobin (hgbA) and single-component transferrin receptor (tbpA2) genes, these results support the idea that virulence may depend in part on the ability to utilize iron from hemoglobin and transferrin.…”

Section: Vol 189 2007 Genomic Sequence Analysis Of Haemophilus Somnsupporting

confidence: 84%

“…H. somnus 129Pt had hgbA, but due to a frameshift, it was split into two ORFS (HS_0720 and HS_0721). The hgbA genes of H. somni ovine strains 9L and 3384Y are also frameshifted, and this finding correlates with an inability of these strains to bind and utilize hemoglobin (67); in the ovine strains 9L and 3384Y, the premature stop codon in hgbA occurs much earlier in the sequence (base 642) than in the 129Pt gene (base 2958). The frameshifted H. somnus 129Pt hgbA gene was adjacent to two genes involved in heme utilization (HS_0722 and HS_0723).…”

Section: Vol 189 2007 Genomic Sequence Analysis Of Haemophilus Somnmentioning

confidence: 99%

“…For example, bovine H. somnus isolates produce hemoglobin receptors that mediate the uptake of iron from bovine hemoglobin but not from ovine, porcine, or human hemoglobin (67). There is evidence that the ability of H. somnus isolates to cause infection in ruminants may depend on the host specificity of their transferrin receptors (74).…”

Section: Vol 189 2007 Genomic Sequence Analysis Of Haemophilus Somnmentioning

confidence: 99%

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Complete Genome Sequence of Haemophilus somnus ( Histophilus somni ) Strain 129Pt and Comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd

et al. 2007

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Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. somnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. somnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.Haemophilus somnus (Histophilus somni), a member of the family Pasteurellaceae (47), can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Commensal H. somnus strains colonize and reside relatively harmlessly in the upper respiratory and reproductive tracts, while pathogenic strains spread systemically and cause diseases such as pneumonia, thrombotic meningoencephalitis, myocarditis, septicemia, arthritis, and abortion (10,34,41,42). Here, we report the genome sequence of H. somnus 129Pt, a commensal preputial isolate. We compared H. somnus 129Pt with the finished genomes of Haemophilus influenzae Rd, an avirulent laboratory strain that can invade certain human epithelial cell lines in culture (12), and Haemophilus ducreyi 35000HP, which causes the human sexually transmitted disease chancroid. H. somnus 129Pt and H. influenzae Rd are moderately related (14, 25), do not cause disease, and colonize mucosal niches in different hosts. Phylogenetic analyses have shown that H. ducreyi is not closely related to members of the genus Haemophilus (1, 14, 30) but is a member of the Pasteurellaceae that colonizes genital mucosal surfaces. Its surface lipooligosaccharides (LOS) are similar in structure to those from Haemophilus and Neisseria strains (8), and it occupies a (mucosal) niche similar to that of H. somnus but in a different host. Therefore, the identification of common genes that these organisms share as well as genes unique to each organism may provide some clues about their host specificities and niche preferences.Through a genome-wide comparative analysis of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP, we found similarities and differences among these organisms in terms of the numbers and compositions o...

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Section: Vol 189 2007 Genomic Sequence Analysis Of Haemophilus Somnsupporting

confidence: 84%

Section: Vol 189 2007 Genomic Sequence Analysis Of Haemophilus Somnmentioning

confidence: 99%

Section: Vol 189 2007 Genomic Sequence Analysis Of Haemophilus Somnmentioning

confidence: 99%

See 1 more Smart Citation

Complete Genome Sequence of Haemophilus somnus ( Histophilus somni ) Strain 129Pt and Comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd

et al. 2007

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“…These compounds could also be used by Histophilus in its methane, nitrogen, and sulfur metabolism, contributing to the context of infection. Furthermore, competition could occur between Histophilus, Acinetobacter, and Corynebacterium over the use of iron, because they have been shown to use iron in some metabolic processes and/or pathogens (Tremblay et al, 2006;Follmann et al, 2009;Zimbler et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease

Rodrigues¹,

Kästle²,

Coutinho³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The microbial community of the reproductive apparatus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic information about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal surface mucosal secretions were collected from five healthy cows and five cows that showed symptoms of reproductive disorders. Following high-throughput sequencing of the isolated microbial DNA, data were processed using the Mothur software to remove low-quality sequences and chimeras, and released to the Ribosomal Database Project for classification of operational taxonomic units (OTUs). Local BLASTn was performed and results were 6519 Vaginal microbiota diversity of healthy and diseased cattle ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6518-6528 (2015) loaded into the MEGAN program for viewing profiles and taxonomic microbial attributes. The control profile comprised a total of 15 taxa, with Bacteroides, Enterobacteriaceae, and Victivallis comprising the highest representation of OTUs; the reproductive disorder-positive profile comprised 68 taxa, with Bacteroides, Enterobacteriaceae, Histophilus, Victivallis, Alistipes, and Coriobacteriaceae being the taxa with the most OTU representation. A change was observed in both the community composition as well as in the microbial attributes of the profiles, suggesting that a relationship might exist between the pathogen and representative taxa, reflecting the production of metabolites to disease progression.

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“…Acquisition of haemoglobin-bound iron has been studied in Hist. somni (Tremblay et al, 2006), P. multocida (Harper et al, 2006), Haemophilus ducreyi (Elkins et al, 1995), Aggregatibacter actinomycetemcomitans (Hayashida et al, 2002), Actinobacillus pleuropneumoniae (Jacques, 2004) and A. suis (Bahrami & Niven, 2005). Relevance of iron in pathogenesis has been demonstrated since it has been shown that irondeficient diets enhance resistance to infection, whereas hyperferremia increases host susceptibility to infection (Litwin & Calderwood, 1993).…”

Section: Introductionmentioning

confidence: 99%

Identification of iron-acquisition proteins ofAvibacterium paragallinarum

et al. 2009

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When Avibacterium paragallinarum reference strain 0083 (serovar A) was grown in an iron-restricted culture medium, the expression of the 60, 68 and 93 kDa outer membrane proteins increased as compared with normal media. Sera of chickens experimentally infected with Av. paragallinarum recognized these ironrestriction induced proteins, suggesting their expression in vivo. The three outer membrane proteins were identified as transferrin receptor and iron transport proteins by mass spectroscopy and a search in sequence databases. As these proteins have been reported to be regulated by the Fur protein in many bacteria, we investigated, through molecular methods, the presence of the fur gene in Av. paragallinarum. A candidate fur gene of Av. paragallinarum was amplified by polymerase chain reaction using complementary primers to conserved regions of fur gene sequences from members of the Pasteurellaceae family. The nucleotide sequence of the cloned gene, from ATG to TAA stop codon, was 453 base pairs in length and the deduced amino acid sequence showed 94% identity with Fur sequences of Actinobacillus pleuropneumoniae and Haemophilus ducreyi. The Av. paragallinarum deduced Fur protein (17.8 kDa) amino acid sequence contains the N-terminal helixÁturnÁhelix DNA-binding domain and the two iron-binding sites in the C-terminal end, typical of other described Fur proteins. The study of iron-restriction-induced proteins and the mechanism regulating their expression could lead to an understanding of the responses of Av. paragallinarum to survive in an iron-restricted environment on host mucosal surfaces.

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Acquisition of haemoglobin-bound iron by Histophilus somni

Cited by 5 publications

References 132 publications

Complete Genome Sequence of Haemophilus somnus ( Histophilus somni ) Strain 129Pt and Comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd

Complete Genome Sequence of Haemophilus somnus ( Histophilus somni ) Strain 129Pt and Comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd

Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease

Identification of iron-acquisition proteins ofAvibacterium paragallinarum

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