Acquisition of Desiccation Tolerance by Cultured Carrot Cells upon Ectopic Expression of C-ABI3, a Carrot Homolog of ABI3

Shiota, Hajime; Kamada, Hiroshi

doi:10.1016/s0176-1617(00)80166-2

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…A homolog of this gene in carrot was isolated and named C-ABI3 (Shiota et al 1998). This gene is mainly expressed in embryonic tissue and positively regulates the expression of the ECP genes (Shiota et al 1998;Shiota and Kamada 2000).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells

Kikuchi

Sanuki

Higashi

et al. 2005

Planta

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Studies of carrot embryogenesis have suggested that abscisic acid (ABA) is involved in somatic embryogenesis. A relationship between endogenous ABA and the induction of somatic embryogenesis was demonstrated using stress-induced system of somatic embryos. The embryonic-specific genes C-ABI3 and embryogenic cell proteins (ECPs) were expressed during stress treatment prior to the formation of somatic embryos. The stress-induction system for embryogenesis was clearly distinguished by two phases: the acquisition of embryogenic competence and the formation of a somatic embryo. Somatic embryo formation was inhibited by the application of fluridone (especially at 10(-4) M), a potent inhibitor of ABA biosynthesis, during stress treatment. The inhibitory effect of fluridone was nullified by the simultaneous application of fluridone and ABA. The level of endogenous ABA increased transiently during stress. However, somatic embryogenesis was not significantly induced by the application of only ABA to the endogenous level, in the absence of stress. These results suggest that the induction of somatic embryogenesis, in particular the acquisition of embryogenic competence, is caused not only by the presence of ABA but also by physiological responses that are directly controlled by stresses.

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Section: Introductionmentioning

confidence: 99%

Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells

Kikuchi

Sanuki

Higashi

et al. 2005

Planta

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“…The duration of exogenous ABA (10 mM) treatment increased the viability of somatic embryos after desiccation, and the effect of ABA on viability was saturated within 10 days of ABA treatment ( Figure 3C). This suggests that it takes 10 days to saturate the accumulation of gene products and osmolytes involved in desiccation tolerance and induced by ABA (Shiota and Kamada 2000). The effects of (ϩ)-ABA and racemic ABA on the viability of desiccated somatic embryos were the same ( Figure 3C).…”

Section: Resultsmentioning

confidence: 82%

Optical isomers of abscisic acid in carrot somatic embryos have the same effect on induction of dormancy and desiccation tolerance

Shiota

Kamada

2008

Plant Biotechnology

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“…Further, ABA-inducible genes have been also isolated and characterized that express specifically in embryos or embryogenic cells [60,61]. During the early embryogenesis stages, a carrot homolog of ABI (C-ABI3) gene appears to regulate the expression of embryogenic cell protein genes, and these proteins later were found to be involved in the process to achieve the somatic cell embryogenic competency [16].…”

Section: Abscisic Acid (Aba)-inducible Genesmentioning

confidence: 99%

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

2018

J App Biol Biotech

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Based on the previous available documents involving molecular events during plant somatic embryogenesis, this report aims to review the advances that have been made for the past several years in the area of molecular mechanism of plant somatic embryogenesis. To begin with, studies suggest that the induction and differentiation of embryos from somatic tissue directly or through callusing involves the interaction of various cellular and molecular factors. Several intra-and extra-cellular proteins such as germins and germins-like proteins, lipid transfer proteins, heatshock proteins, and late embryogenesis abundant proteins are known to regulate the induction of somatic embryos from the somatic cell. Simultaneously, regulation and expression of specific genes such as housekeeping genes OsIAA in rice; hormone-responsive genes Dcarg-1, Dchsp-1, DcECP31, DcEMB1 in carrot; and AtECP63, Mt somatic embryo-related factor 1 in arabidopsis have been identified to play key roles during the process of somatic embryogenesis. These genes are known to express differentially for synthesis of new proteins during induction and development of somatic embryo. In addition, several transcription factors such as leafy cotyledon genes, agamouslike15 (AGL15) gene, ethylene-responsive element-binding protein (EREBPs), knotted1-like homeobox proteins, and RWP-RK group of plant-specific transcription factors are equally known that efficiently control the molecular events of somatic embryogenesis. Further, it is also now established that epigenetic factors such asDNA methylation, histone deacetylation/methylation, and microRNAs also influence the molecular mechanism of plant somatic embryogenesis.

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Acquisition of Desiccation Tolerance by Cultured Carrot Cells upon Ectopic Expression of C-ABI3, a Carrot Homolog of ABI3

Cited by 13 publications

References 22 publications

Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells

Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells

Optical isomers of abscisic acid in carrot somatic embryos have the same effect on induction of dormancy and desiccation tolerance

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

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