Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa

Yokoyama, Keiko; Doi, Yohei; Yamane, Kunikazu; Kurokawa, Hiroshi; Shibata, Naohiro; Shibayama, Keigo; Yagi, Tetsuya; Kato, Haru; Arakawa, Yoshichika

doi:10.1016/s0140-6736(03)14959-8

Cited by 201 publications

(149 citation statements)

References 21 publications

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“…or Streptomyces spp (7). However, we recently reported a novel 16S rRNA methylase, RmtA, that was identified in a P. aeruginosa clinical strain, AR-2 (29 In the present study, we investigated the genetic environments mediating the rmtA genes found in two different strains of P. aeruginosa. The GϩC content of the rmtA gene was 55%, and those of 16S rRNA methylase genes found in aminoglycoside-producing actinomycetes were 64 to 72%.…”

Section: Discussionmentioning

confidence: 92%

“…Strains AR-101 and AR-105 also demonstrated quite different PFGE profiles despite being isolated at the same hospital. Furthermore, the arbakacin resistance profile of AR-2 was transferable to another P. aeruginosa strain by conjugation (29). This suggested that rmtA was mediated by some transferable plasmids in strain AR-2, but we failed to visualize the plasmid either by the method of Kado and Liu (14) or by cesium chloride-ethidium bromide density gradient ultracentrifugation (21).…”

Section: Discussionmentioning

confidence: 99%

“…This strain harbors a novel aminoglycoside resistance gene named rmtA, which encodes a new 16S rRNA methylase (29). Production of 16S rRNA methylase had been reported among aminoglycoside-producing actinomycetes, including Micromonospora spp.…”

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confidence: 99%

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Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Yamane

Doi

Yokoyama

et al. 2004

Antimicrob Agents Chemother

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Nine Pseudomonas aeruginosa strains showing very high levels of resistance to various aminoglycosides have been isolated from clinical specimens in seven separate Japanese hospitals in five prefectures since 1997. These strains harbor the newly identified 16S rRNA methylase gene (rmtA). When an rmtA gene probe was hybridized with genomic DNAs of the nine strains digested with EcoRI, two distinct patterns were observed. The 11.1-and 15.8-kb regions containing the rmtA genes of strains AR-2 and AR-11, respectively, were sequenced and compared. In strain AR-2, a transposase gene-like sequence (sequence 1) and a probable tRNA ribosyltransferase gene (orfA) were located upstream of rmtA, and a Na ؉ /H ؉ antiporter gene-like sequence (sequence 2) was identified downstream of rmtA. This 6.2-kbp insert (the rmtA locus) was flanked by 262-bp ␥ elements. Part of the orfQ gene adjacent to an inverted repeat was found outside of the rmtA locus. In strain AR-11, the rmtA gene and sequence 2 were found, but the 5 end of the orfA gene was truncated and replaced with IS6100. An orfQ-orfI region was present on each side of the rmtA gene in strain AR-11. The G؉C content of the rmtA gene was about 55%, and since the newly identified rmtA gene may well be mediated by some mobile genetic elements such as Tn5041, further dissemination of the rmtA gene could become an actual clinical problem in the near future.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Yamane

Doi

Yokoyama

et al. 2004

Antimicrob Agents Chemother

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“…The first reported 16S rRNA methyltransferase gene armA ( aminoglycoside resistance methyltransferase) was identified in a multiresistant Klebsiella pneumoniae isolated in France (Galimand et al, 2003). A further seven enzymes have been associated with 16S rRNA methylation: rmtA (Yokoyama et al, 2003), rmtB (Doi et al, 2004), rmtC (Wachino et al, 2006a), rmtD (now renamed as rmtD1) , rmtD2 (shares 96.4% amino acid identity with rmtD1) (Tijet et al, 2011), rmtE (Davis et al, 2010) and npmA (Wachino et al, 2007). The deduced amino acid sequences show homology with 16S rRNA methyltransferases produced by Actinomycetales, including Streptomyces and Micromonospora spp.…”

Section: Aminoglycoside Resistance Mediated By 16s Rrna Methylationmentioning

confidence: 99%

16S rRNA Methyltransferases: An Emerging Resistance Mechanism Against Aminoglycosides in Salmonella

Hopkins¹,

González‐Zorn²

2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…Two distinct groups of resistance MTases have been distinguished, based upon their target nucleotide preferences. Five of them -ArmA, RmtA, RmtB, RmtC, and RmtD, that confer resistance to 4,6-but not to 4,5-DOS antibiotics, have been globally found in Enterobacteriacea, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens, Proteus mirabilis and Acinetobacter spp., and they are classified as the Arm MTase family (Aminoglycoside Resistance Methyltransferase) (64)(65)(66)(67)(68)(69). It has been experimentally determined that only two members of the Arm family -ArmA and RmtB, methylate A1405 position at the ribosomal A-site (70), similarly to Kgm MTase family from producers.…”

Section: Pathogenic Bacteriamentioning

confidence: 99%

rRNA Methyltransferases and their Role in Resistance to Antibiotics

Morić¹,

Savic²,

Ilić-Tomič³

et al. 2010

Journal of Medical Biochemistry

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MTaze metiluju nukleinske kiseline (DNK, RNK) i proteine, moduli{u}i tako njihovu aktivnost, funkciju i strukturnu organizaciju. Metilacija G1405 ili A1408 baza u 16S rRNK mikroorganizama koji proizvode aminoglikozide obezbe |u -je rezistenciju na sopstvene toksi~ne pro izvode. Ovaj mehanizam rezistencije je donedavno bio opi san samo kod proizvo|a~a antibiotika. Od 2003. godine i kod patogenih bakterija bele`i se neprestan porast rezi sten cije na aminoglikozide putem ovog mehanizma, {to predstavlja veliku pretnju efikasnoj upotrebi aminoglikozida u klini~koj praksi. Jedno od mogu}ih re{enja problema le`i u razvoju novih jedinjenja koja bi efikasno delovala na nova mesta u okviru ribozoma. Drugi pristup re{avanju ovog problema uklju~uje razvoj inhibitora MTaza odgovornih za rezisten ciju, sa idejom da se onemogu}i modifikacija bakte rijske rRNK i na taj na~in vrati terapeutska efikasnost postoje}im aminogliko zidima. Fundamentalna istra`ivanja vezana za proteinsku ekspresiju, potpuno razumevanje mehanizma rezistencije kao i razre{enje tercijarne strukture proteina su neophodan preduslov za primenu inhibitora 16S rRNK MTaza u medicini.

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Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa

Cited by 201 publications

References 21 publications

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

16S rRNA Methyltransferases: An Emerging Resistance Mechanism Against Aminoglycosides in Salmonella

rRNA Methyltransferases and their Role in Resistance to Antibiotics

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