Acoustic Wave Sensors

Grate, Jay W.; Frye, G.C.

doi:10.1002/1616-8984(199610)2:1<37::aid-seup37>3.0.co;2-f

Cited by 134 publications

(57 citation statements)

References 225 publications

(371 reference statements)

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“…To facilitate characterization work, model systems are often studied with surface-sensitive analytical tools [24]. A range of techniques exist to probe biological thin film interfaces, and can measure acoustic [25], optical [26], spectroscopic [27], electrical [28], chemical [29], and morphological properties [30]. With this wealth of options, there are many analytical strategies to choose from in order to characterize the specific target interaction.…”

Section: Introductionsupporting

confidence: 40%

Model Membrane Platforms for Biomedicine: Case Study on Antiviral Drug Development

Jackman

Cho

2012

Biointerphases

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As one of the most important interfaces in cellular systems, biological membranes have essential functions in many activities such as cellular protection and signaling. Beyond their direct functions, they also serve as scaffolds to support the association of proteins involved in structural support, adhesion, and transport. Unfortunately, biological processes sometimes malfunction and require therapeutic intervention. For those processes which occur within or upon membranes, it is oftentimes difficult to study the mechanism in a biologically relevant, membranous environment. Therefore, the identification of direct therapeutic targets is challenging. In order to overcome this barrier, engineering strategies offer a new approach to interrogate biological activities at membrane interfaces by analyzing them through the principles of the interfacial sciences. Since membranes are complex biological interfaces, the development of simplified model systems which mimic important properties of membranes can enable fundamental characterization of interaction parameters for such processes. We have selected the hepatitis C virus (HCV) as a model viral pathogen to demonstrate how model membrane platforms can aid antiviral drug discovery and development. Responsible for generating the genomic diversity that makes treating HCV infection so difficult, viral replication represents an ideal step in the virus life cycle for therapeutic intervention. To target HCV genome replication, the interaction of viral proteins with model membrane platforms has served as a useful strategy for target identification and characterization. In this review article, we demonstrate how engineering approaches have led to the discovery of a new functional activity encoded within the HCV nonstructural 5A protein. Specifically, its N-terminal amphipathic, α-helix (AH) can rupture lipid vesicles in a size-dependent manner. While this activity has a number of exciting biotechnology and biomedical applications, arguably the most promising one is in antiviral medicine. Based on the similarities between lipid vesicles and the lipid envelopes of virus particles, experimental findings from model membrane platforms led to the prediction that a range of medically important viruses might be susceptible to rupturing treatment with synthetic AH peptide. This hypothesis was tested and validated by molecular virology studies. Broad-spectrum antiviral activity of the AH peptide has been identified against HCV, HIV, herpes simplex virus, and dengue virus, and many more deadly pathogens. As a result, the AH peptide is the first in class of broad-spectrum, lipid envelope-rupturing antiviral agents, and has entered the drug pipeline. In summary, engineering strategies break down complex biological systems into simplified biomimetic models that recapitulate the most important parameters. This approach is particularly advantageous for membrane-associated biological processes because model membrane platforms provide more direct characterization of target interactions than is possible with other methods. Consequently, model membrane platforms hold great promise for solving important biomedical problems and speeding up the translation of biological knowledge into clinical applications.

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Section: Introductionsupporting

confidence: 40%

Model Membrane Platforms for Biomedicine: Case Study on Antiviral Drug Development

Jackman

Cho

2012

Biointerphases

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“…With scanning-force microscopy (SFM) it is possible to conduct direct observations of the topography on a nanometer scale. Additionally the monitoring of the spatial distribution of adsorbed proteins is possible without averaging over larger areas as done by ellipsometry [8] or quartz crystal microbalance measurements [9,10]. By conducting measurements in a flow-through setup in a liquid cell information on the time resolved changes occurring during adsorption can be achieved.…”

Section: Introductionsupporting

confidence: 38%

Scanning-force techniques to monitor time-dependent changes in topography and adhesion force of proteins on surfaces

2003

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Scanning-force microscopy (SFM) investigations were conducted to probe the influences of the interactions of proteins with surfaces relevant in medicine. These interactions are an important feature in the area of biofilm formation. The adsorption of proteins leads to changes in topography, which was monitored for the build up of protein layers of hen egg-white lysozyme and bovine serum albumin (BSA) on mica in real time in phosphate-buffered aqueous solution over a time period of 10 min. Phase imaging was additionally applied to compare material contrasts and to evaluate this method for further application in this field. The adhesion forces that develop on a time scale below 20 s between a protein-modified SFM tip and titanium surfaces (TiO(2), TiAl6V4 and TiAl6Nb7) were investigated. The influences of the parameters loading force and interaction time between the protein and the surface were monitored as well as the influence of protein structure. The interaction time dependency of the adhesion force could be described with a kinetic model of two consecutive first-order reactions. For the maximal adhesion force a correlation to the ratio of the amino acids cysteine, proline and glycine has been proposed.

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“…As a result, the acoustic energy is restricted at the surface of the device in the range of acoustic wavelength [30]. Subsequently, SAW based devices are very sensitive in terms of any changes over the surface of the device, such as mass loading, viscosity and conductivity changes [31][32][33].…”

Section: Introductionsupporting

confidence: 40%

Acoustic wave based biosensor to study electroacoustic based detection of progressive (SW-48) colon cancer cells from primary (HT-29) cells

Dahmardeh

Sheybanifar

Gharooni

et al. 2015

Sensors and Actuators A: Physical

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Acoustic Wave Sensors

Cited by 134 publications

References 225 publications

Model Membrane Platforms for Biomedicine: Case Study on Antiviral Drug Development

Model Membrane Platforms for Biomedicine: Case Study on Antiviral Drug Development

Scanning-force techniques to monitor time-dependent changes in topography and adhesion force of proteins on surfaces

Acoustic wave based biosensor to study electroacoustic based detection of progressive (SW-48) colon cancer cells from primary (HT-29) cells

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