Acoustic tracheal rupture provides insights into larval mosquito respiration

Nyberg, Herbert; Muto, Kunihiro

doi:10.1038/s41598-020-59321-8

Cited by 5 publications

(7 citation statements)

References 35 publications

(45 reference statements)

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“…It is clear that this procedure would negatively affect not only mosquitoes, but also the rest of the organisms living in the same environment. More recently, Nyberg and Muto (2020) investigated the mechanism of action of what authors called “mosquito acoustic larviciding” . They reported that their acoustic treatment provoked the tracheal system a series of what they reported as “previously unobserved phenomena” According to Nyberg and Muto (2020), these phenomena would be difficult to explain based on the present knowledge of mosquito respiration, eventually concluding that the respiratory function in mosquitoes is far from being completely understood.…”

Section: Discussionmentioning

confidence: 99%

Challenging popular belief, mosquito larvae breathe underwater

Costa

Leonardi

Giraud

et al. 2023

Preprint

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It is taken for granted that immature mosquito only breathe atmospheric air through their siphons. However, there is no quantitative study that demonstrated it. We analysed the survival of the last instar larvae of Aedes aegypti fully submerged at different temperatures, and measured oxygen consumption from air and dissolved in water, of larvae and pupae of this species under different conditions. Results revealed that under water, larvae survived much longer than expected, reaching 50% mortality only after 58, 10, and 5 days at 15, 25 and 35 degrees C, respectively. Interestingly, whereas we registered moults to pupae in larvae with access to air, individuals kept submerged never moulted. When remaining at the water surface, larvae obtained 12.72% of O2 from the water, while pupae only 5.32%. When completely submerged, larvae consumed less oxygen than in contact with the surface, but enough for surviving, while pupae did not. At both media, temperature affected larvae respiration rate, with relatively close Q10 values. In the related species, Ae. albopictus, a similar pattern of O2 consumption were observed. Larvae got 12.14% of their oxygen from the water. Interestingly, no significant differences in total O2 consumption were found between water O2 consumption, when Ae. albopictus larvae were submerged, or when they also have access to air (dual O2 consumption). Our findings not only challenge the classical idea that mosquito larvae only breathe atmospheric O2, but also force us to reconsider the potential effectiveness of control methods based on asphyxiating larvae by detaching from water surface.

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Section: Discussionmentioning

confidence: 99%

Challenging popular belief, mosquito larvae breathe underwater

Costa

Leonardi

Giraud

et al. 2023

Preprint

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“…We adopted a technology employing acoustic waves generated by an ultrasound transducer, the Larvasonic apparatus (New Mountain Innovations Inc, Old Lyme, NY, USA) [5]. The transducer distributes acoustic energy uniformly in the horizontal plane and has a 360-degree volume of vertical coverage.…”

Section: Efficacy Of Ultrasonic Treatment At Laboratory Conditionsmentioning

confidence: 99%

“…Mosquitoes (Diptera: Culicidae) transmit several of the most relevant life-threatening infectious diseases worldwide, including malaria, different types of encephalitis, lymphatic filariasis, West Nile virus, chikungunya, yellow fever, as well as Zika and dengue virus [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic Technology Applied against Mosquito Larvae

et al. 2020

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The effective management of mosquito vectors is a timely challenge for medical and veterinary entomology. In this study, we evaluated the acoustic Larvasonic device to control young instars of the mosquito Aedes aegypti in diverse freshwater environments. Under laboratory conditions, we investigated the effect of exposure time and distance from the transducer on the mortality of larvae and pupae of Ae. aegypti. Furthermore, we evaluated the effectiveness of the ultrasound window of the electromagnetic spectrum under different field conditions. Results showed that first and second instar larvae were more sensitive to the frequency range of 18–30 kHz of the Larvasonic device. Ultrasonic waves applied for 180 s at a frequency from 18 to 30 kHz caused 100% larval mortality at a distance of 60 cm from the transducer. No mortality was observed in the non-target copepod Megacyclops formosanus. The exposure to the soundwaves produced by the acoustic larvicidal device over different distances effectively damaged Ae. aegypti through destruction of the larval dorsal tracheal trunk, thorax and abdomen. Overall, results indicated that the Larvasonic device tested can provide an alternative tool to reduce young instar populations of Ae. aegypti, without any effects on non-target aquatic invertebrates like copepods. It turned out to be a useful device for mosquito biocontrol. This technology has a relevant potential to fight the spread of mosquito-borne diseases.

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“…We synthesized a N 1 -(7-chloroquinoline-4-yl) ethane-1,2-diamine derivative by the method of Shafi et al 8 against A. stephensi providing no harmful impacts on the environment as well on non-target organisms (see Nyberg et al 27 ). As the plasmodium parasite becomes more resistant to quinoline based anti-malarial drugs, it becomes even more important to design a potent anti-malarial molecule 28 , 29 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of new series of quinoline derivatives with insecticidal effects on larval vectors of malaria and dengue diseases

Murugan

Panneerselvam

Subramaniam

et al. 2022

Sci Rep

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Mosquito borne diseases are on the rise because of their fast spread worldwide and the lack of effective treatments. Here we are focusing on the development of a novel anti-malarial and virucidal agent with biocidal effects also on its vectors. We have synthesized a new quinoline (4,7-dichloroquinoline) derivative which showed significant larvicidal and pupicidal properties against a malarial and a dengue vector and a lethal toxicity ranging from 4.408 µM/mL (first instar larvae) to 7.958 µM/mL (pupal populations) for Anopheles stephensi and 5.016 µM/mL (larva 1) to 10.669 µM/mL (pupae) for Aedes aegypti. In-vitro antiplasmodial efficacy of 4,7-dichloroquinoline revealed a significant growth inhibition of both sensitive strains of Plasmodium falciparum with IC50 values of 6.7 nM (CQ-s) and 8.5 nM (CQ-r). Chloroquine IC50 values, as control, were 23 nM (CQ-s), and 27.5 nM (CQ-r). In vivo antiplasmodial studies with P. falciparum infected mice showed an effect of 4,7-dichloroquinoline compared to chloroquine. The quinoline compound showed significant activity against the viral pathogen serotype 2 (DENV-2). In vitro conditions and the purified quinoline exhibited insignificant toxicity on the host system up to 100 µM/mL. Overall, 4,7-dichloroquinoline could provide a good anti-vectorial and anti-malarial agent.

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Acoustic tracheal rupture provides insights into larval mosquito respiration

Cited by 5 publications

References 35 publications

Challenging popular belief, mosquito larvae breathe underwater

Challenging popular belief, mosquito larvae breathe underwater

Ultrasonic Technology Applied against Mosquito Larvae

Synthesis of new series of quinoline derivatives with insecticidal effects on larval vectors of malaria and dengue diseases

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