Acoustic separation in plastic microfluidics for rapid detection of bacteria in blood using engineered bacteriophage

Dow, Paul C.; Kotz, Kenneth T.; Gruszka, S.; Holder, Justin; Fiering, Jason

doi:10.1039/c7lc01180f

Cited by 89 publications

(63 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, bulk acoustophoresis was used to identify bacteria using a bacteriophage-based luminescence assay in a microfluidic chip [23]. To demonstrate the effectiveness of the acoustophoretic system, three clinically relevant species of bacteria were tested, namely, Pseudomonas aeruginosa, S. aureus and E. coli.…”

Section: Methods Involving Sound Wave Acoustophoresismentioning

confidence: 99%

New solutions to capture and enrich bacteria from complex samples

Sande

Çaykara

Silva

et al. 2020

Med Microbiol Immunol

View full text Add to dashboard Cite

Current solutions to diagnose bacterial infections though reliable are often time-consuming, laborious and need a specific laboratory setting. There is an unmet need for bedside accurate diagnosis of infectious diseases with a short turnaround time. Moreover, low-cost diagnostics will greatly benefit regions with poor resources. Immunoassays and molecular techniques have been used to develop highly sensitive diagnosis solutions but retaining many of the abovementioned limitations. The detection of bacteria in a biological sample can be enhanced by a previous step of capture and enrichment. This will ease the following process enabling a more sensitive detection and increasing the possibility of a conclusive identification in the downstream diagnosis. This review explores the latest developments regarding the initial steps of capture and enrichment of bacteria from complex samples with the ultimate goal of designing low cost and reliable diagnostics for bacterial infections. Some solutions use specific ligands tethered to magnetic constructs for separation under magnetic fields, microfluidic platforms and engineered nano-patterned surfaces to trap bacteria. Bulk acoustics, advection and nano-filters comprise some of the most innovative solutions for bacteria enrichment.

show abstract

Section: Methods Involving Sound Wave Acoustophoresismentioning

confidence: 99%

New solutions to capture and enrich bacteria from complex samples

Sande

Çaykara

Silva

et al. 2020

Med Microbiol Immunol

View full text Add to dashboard Cite

show abstract

“…Although BAW‐based devices generally enable handling larger fluid volumes and throughputs, [ 51–53 ] SAW devices are advantageous for precise micromanipulation since they can achieve higher frequencies, [ 45,54 ] can readily localize the acoustic fields in specific regions, [ 38,55,56 ] and are a well‐established method for the precise spatial control of particles. [ 45 ] Periodic patterns of suspended particles are typically formed using standing surface acoustic waves (SSAWs), [ 57–60 ] though we recently demonstrated particle patterning using traveling SAWs via near‐field interference patterns formed near channel boundaries and in‐channel features.…”

Section: Introductionmentioning

confidence: 99%

Massively Multiplexed Submicron Particle Patterning in Acoustically Driven Oscillating Nanocavities

Tayebi

O’Rorke

Wong

et al. 2020

Small

View full text Add to dashboard Cite

high-throughput particle manipulation for preparation, enrichment, and quality control. For example, the size distribution of synthesized nanoparticle catalysts determines their catalysis efficiency. [7,8] In biomedical applications, exosomes, which are submicron extracellular vesicles [9][10][11] containing DNA, microRNAs, and proteins, are a potential source of diagnostic biomarkers. [12][13][14][15] Suitable nanoparticle manipulation tools would enable sizebased sorting/filtering, or manipulation toward downstream activities such as multiplexed diagnostics, nanoelectronic/ robotic constructions, [16,17] and nanoparticle-based drug delivery systems. [18] Conventional particle separation techniques [19] are based on differential density and size, e.g., ultracentrifugation, [20] ultrafiltration, [21] and size exclusion chromatography. [22] Although impressive progress has been made in the last decade, many nanoparticle manipulation tools suffer from high cost, low throughput, and specimen damage. [23][24][25] Scalable nano-and submicron particle patterning has thus traditionally relied on self-assembly methods [26] or oligomer templating [27] however, these are largely unable to produce deterministic singlenanoparticle positioning, they are irreversible, and they require specific reagents or lengthy processing. [28][29][30] Moreover, drug delivery systems which incorporate efficient nanoparticle concentration are creating new avenues for cancer treatment. [31,32] There is, therefore, a pressing need to develop a fast, precise, and scalable method for nano-and submicron scale manipulation.Microfluidic devices have emerged as a viable platform for particle manipulation using magnetic, [33] optoelectronic, [34] plasmonic, [35] electrokinetic, [36] and hydrodynamic [37] forces. Applying these principles at the nanoscale instead of the microscale, however, can result in far smaller force magnitudes, poor separation efficiency, and channel clogging. [38] Furthermore, most of these manipulation methods are constrained by their dependence on particle polarizability, the need for low conductivity medium (which is often not biocompatible), and heating of the surrounding fluid. Optical tweezers [39,40] provide the highest degree of spatial resolution but they are usually applied in static fluids, owing to the relatively small forces that can be generated, [41] and only small numbers of nanoparticles can be manipulated at any one time (those within the Nanoacoustic fields are a promising method for particle actuation at the nanoscale, though THz frequencies are typically required to create nanoscale wavelengths. In this work, the generation of robust nanoscale force gradients is demonstrated using MHz driving frequencies via acoustic-structure interactions. A structured elastic layer at the interface between a microfluidic channel and a traveling surface acoustic wave (SAW) device results in submicron acoustic traps, each of which can trap individual submicron particles. The acoustically driven deformation of nanocavities gi...

show abstract

“…Therefore, there is a drive in finding new fabrication methods and materials to decrease the cost and time for fabrication of bulk acoustic wave devices. There are a few reports of bulk acoustic wave devices fabricated in plastics [9,10]. Plastic microfluidic chips have the advantage of being suitable for mass production.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Silicon Microfluidic Chips for Acoustic Particle Focusing Using Direct Laser Writing

et al. 2020

View full text Add to dashboard Cite

We have developed a fast and simple method for fabricating microfluidic channels in silicon using direct laser writing. The laser microfabrication process was optimised to generate microfluidic channels with vertical walls suitable for acoustic particle focusing by bulk acoustic waves. The width of the acoustic resonance channel was designed to be 380 µm, branching into a trifurcation with 127 µm wide side outlet channels. The optimised settings used to make the microfluidic channels were 50% laser radiation power, 10 kHz pulse frequency and 35 passes. With these settings, six chips could be ablated in 5 h. The microfluidic channels were sealed with a glass wafer using adhesive bonding, diced into individual chips, and a piezoelectric transducer was glued to each chip. With acoustic actuation at 2.03 MHz a half wavelength resonance mode was generated in the microfluidic channel, and polystyrene microparticles (10 µm diameter) were focused along the centre-line of the channel. The presented fabrication process is especially interesting for research purposes as it opens up for rapid prototyping of silicon-glass microfluidic chips for acoustofluidic applications.

show abstract

Acoustic separation in plastic microfluidics for rapid detection of bacteria in blood using engineered bacteriophage

Cited by 89 publications

References 45 publications

New solutions to capture and enrich bacteria from complex samples

New solutions to capture and enrich bacteria from complex samples

Massively Multiplexed Submicron Particle Patterning in Acoustically Driven Oscillating Nanocavities

Fabrication of Silicon Microfluidic Chips for Acoustic Particle Focusing Using Direct Laser Writing

Contact Info

Product

Resources

About