Acoustic Rhinometry Evaluation of Nasal Response to Histamine and Antigen in Guinea Pigs

Abstract

The effects of antigen and histamine on the changes of nasal passage patency in 112 guinea pigs with or without allergic rhinitis were evaluated by acoustic rhinometry. The percent change of volume from the nostril to 2 cm into the nasal cavity showed significant reduction of 31.10 +/- 4.11% at 10 minutes and 31.10 +/- 4.11% at 30 minutes after antigen challenge in sensitized guinea pigs. The pretreatment with ketotifen, an H1-histamine receptor antagonist as well as mast cell stabilizing drug, blocked dose-dependently the effects of antigen on those changes in volume. Furthermore, instillation of 10(4) micrograms/mL histamine reduced significantly nasal passage patency to 33.77 +/- 4.63% at 10 minutes and 42.76 +/- 3.32% at 30 minutes after challenge compared with that before challenge and ketotifen inhibited the effects of histamine, which indicated that histamine is an important mediator of allergic upper airway responses in guinea pigs. These results show that acoustic rhinometry is a useful technique to assess the nasal blockade in allergic guinea pigs.

Nasal congestion model in Brown Norway rats and the effects of some H1-antagonists

“…In addition, it has been reported that chlorpheniramine blocked early-phase nasal congestion in sensitized dogs [5]. Although chlorpheniramine and ketotifen inhibited the increase in Penh 1 h after the TDI challenge, ketotifen showed a somewhat stronger effect [3]. As for epinastine, the drug exhibited a more potent and longer lasting effect than chlorpheniramine.…”

“…One of the typical symptoms in allergic rhinitis is nasal congestion. Nasal congestion associated with allergic rhinitis may be due to the increased nasal blood flow causing increased filling and distention of the venous erectile tissue, which develops an edematous swelling of the nasal mucosal membrane [3]. Since nasal congestion in human beings is a subjective symptom, it is therefore difficult to evaluate in animals.…”

Objective measurement of nasal airway dimensions using acoustic rhinometry: methodological and clinical aspects

“…Based on a receptor binding assay and measurements by acoustic rhinometry, it is suggested that sensitization with allergen increases the number of histamine H1 receptors. The increased number of H1 receptors in the nasal mucosa of sensitized guinea pigs may be one of the causes of nasal hyperresponsiveness to antigen (434, 435). The vascular effects of sensory peptides have also been examined (436).…”

Acoustic rhinometry in dog and cat compared with a fluid-displacement method and magnetic resonance imaging

“…The method was first used in laboratory animals (guinea pigs) in 1994 (23). Since then, a small number of studies have used AR to describe the effect of pharmacological interventions on the nasal mucosa in guinea pigs (11,12,(19)(20)(21)24).…”