Acoustic Radiation or Cavitation Forces From Therapeutic Ultrasound Generate Prostaglandins and Increase Mesenchymal Stromal Cell Homing to Murine Muscle

Lorsung, Rebecca M.; Rosenblatt, Robert; Cohen, Gadi; Frank, Joseph A.; Burks, Scott R.

doi:10.3389/fbioe.2020.00870

Cited by 11 publications

(4 citation statements)

References 26 publications

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“…These parameters represented the lowest ultrasound intensity where pro-inflammatory, anti-tumor molecular and cellular responses were observed. Additionally, we recently reported that these sonication parameters generated endogenous microbubble formation and cavitation in skeletal muscle [ 61 ]. We further demonstrated that inflammatory molecular responses in skeletal muscle did not specifically require cavitation, but that such responses were more sensitive to cavitation than ARF.…”

Section: Discussionmentioning

confidence: 99%

Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes

Cohen

Chandran

Lorsung

et al. 2021

Cancers

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Focused ultrasound (FUS) has shown promise as a non-invasive treatment modality for solid malignancies. FUS targeting to tumors has been shown to initiate pro-inflammatory immune responses within the tumor microenvironment. Pulsed FUS (pFUS) can alter the expression of cytokines, chemokines, trophic factors, cell adhesion molecules, and immune cell phenotypes within tissues. Here, we investigated the molecular and immune cell effects of pFUS on murine B16 melanoma and 4T1 breast cancer flank tumors. Temporal changes following sonication were evaluated by proteomics, RNA-seq, flow-cytometry, and histological analyses. Proteomic profiling revealed molecular changes occurring over 24 h post-pFUS that were consistent with a shift toward inflamed tumor microenvironment. Over 5 days post-pFUS, tumor growth rates were significantly decreased while flow cytometric analysis revealed differences in the temporal migration of immune cells. Transcriptomic analyses following sonication identified differences in gene expression patterns between the two tumor types. Histological analyses further demonstrated reduction of proliferation marker, Ki-67 in 4T1, but not in B16 tumors, and activated cleaved-caspase 3 for apoptosis remained elevated up to 3 days post-pFUS in both tumor types. This study revealed diverse biological mechanisms following pFUS treatment and supports its use as a possible adjuvant to ablative tumor treatment to elicit enhanced anti-tumor responses and slow tumor growth.

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Section: Discussionmentioning

confidence: 99%

Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes

Cohen

Chandran

Lorsung

et al. 2021

Cancers

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“… 94 Experiments by Rebecca M. Lorsung et al show that cavitation forces or acoustic radiation from low-intensity pulsed ultrasound (LIPUS) can shape the microenvironment that supports MSC tropism. 91 …”

Section: Factors Influencing the Homing Processmentioning

confidence: 99%

Bioactive Materials That Promote the Homing of Endogenous Mesenchymal Stem Cells to Improve Wound Healing

Jiang,

Chen,

Huang

et al. 2024

IJN

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Endogenous stem cell homing refers to the transport of endogenous mesenchymal stem cells (MSCs) to damaged tissue. The paradigm of using well-designed biomaterials to induce resident stem cells to home in to the injured site while coordinating their behavior and function to promote tissue regeneration is known as endogenous regenerative medicine (ERM). ERM is a promising new avenue in regenerative therapy research, and it involves the mobilizing of endogenous stem cells for homing as the principal means through which to achieve it. Comprehending how mesenchymal stem cells home in and grasp the influencing factors of mesenchymal stem cell homing is essential for the understanding and design of tissue engineering. This review summarizes the process of MSC homing, the factors influencing the homing process, analyses endogenous stem cell homing studies of interest in the field of skin tissue repair, explores the integration of endogenous homing promotion strategies with cellular therapies and details tissue engineering strategies that can be used to modulate endogenous homing of stem cells. In addition to providing more systematic theories and ideas for improved materials for endogenous tissue repair, this review provides new perspectives to explore the complex process of tissue remodeling to enhance the rational design of biomaterial scaffolds and guide tissue regeneration strategies.

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“…pHIFU uses short (1-100 ms) pulses delivered at low duty cycle (<5%) and relatively low frequency (around 1 MHz) to avoid thermal effects and promote cavitation [1]–[8]. This regime was explored in conjunction with several clinical applications, including tumor permeabilization to enhance passive diffusion of systemically administered drugs [1], [2], [9], and inducing changes in tumor and benign tissue microenvironment [3]–[6]. The pHIFU treatments are commonly performed under real-time ultrasound (US) imaging guidance; conventional B-mode imaging with a probe integrated coaxially into the HIFU transducer is typically used for treatment planning and targeting.…”

Section: Introductionmentioning

confidence: 99%

“…pHIFU uses short (1-100 ms) pulses delivered at low duty cycle (<5%) and relatively low frequency (around 1 MHz) to avoid thermal effects and promote cavitation [1]- [8]. This regime was explored in conjunction with several clinical applications, including tumor permeabilization to enhance passive diffusion of systemically administered drugs [1], [2], [9], and inducing changes in tumor and benign tissue microenvironment [3]- [6].…”

Section: Introductionmentioning

confidence: 99%

Dynamic Mode Decomposition for Transient Cavitation Bubbles Imaging in Pulsed High Intensity Focused Ultrasound Therapy

Song,

Sapozhnikov,

Khokhlova

et al. 2024

Preprint

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Pulsed high-intensity focused ultrasound (pHIFU) can induce sparse de novo inertial cavitation without the introduction of exogenous contrast agents, promoting mild mechanical disruption in targeted tissue. Because the bubbles are small and rapidly dissolve after each HIFU pulse, mapping transient bubbles and obtaining real-time quantitative metrics correlated to tissue damage are challenging. Prior work introduced Bubble Doppler, an ultrafast power Doppler imaging method as a sensitive means to map cavitation bubbles. The main limitation of that method was its reliance on conventional wall filters used in Doppler imaging and optimized for imaging blood flow rather than transient scatterers. This study explores Bubble Doppler enhancement using dynamic mode decomposition (DMD) of a matrix created from a Doppler ensemble for mapping and extracting the characteristics of transient cavitation bubbles. DMD was first tested in silico with a numerical dataset mimicking the spatiotemporal characteristics of backscattered signal from tissue and bubbles. The performance of DMD filter was compared to other widely used Doppler wall filters - singular value decomposition (SVD) and infinite impulse response (IIR) highpass filter. DMD was then applied to an ex vivo tissue dataset where each HIFU pulse was immediately followed by a plane wave Doppler ensemble. In silico DMD outperformed SVD and IIR high pass filter and ex vivo provided physically interpretable images of the modes associated with bubbles and their corresponding temporal decay rates. These DMD modes can be trackable over the duration of pHIFU treatment using k-means clustering method, resulting in quantitative indicators of treatment progression.

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Acoustic Radiation or Cavitation Forces From Therapeutic Ultrasound Generate Prostaglandins and Increase Mesenchymal Stromal Cell Homing to Murine Muscle

Cited by 11 publications

References 26 publications

Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes

Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes

Bioactive Materials That Promote the Homing of Endogenous Mesenchymal Stem Cells to Improve Wound Healing

Dynamic Mode Decomposition for Transient Cavitation Bubbles Imaging in Pulsed High Intensity Focused Ultrasound Therapy

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