This paper examines potential prosodic cues for level of formality in Japanese conversational speech using speech data gathered via one-on-one interviews. Based on previous work on the phonetic properties of formality in Japanese (Ofuka et al. 2000, Ito 2002), further studies of Korean (Winter & Grawunder 2012) and Catalan Spanish (Hübscher, Borràs-Comes & Prieto 2017), and on a lab-based pilot study, the study examined properties of f0, intensity, pause frequency, and articulation rate via mixed effects regression models and a functional data analysis (Grabe, Kochanski & Coleman 2007, Ramsay 2006). Analysis of the speech data shows significant relationships between a number of prosodic variables and level of formality, and suggests that some of these relationships may apply cross-linguistically.