Acoustic droplet vaporization for on-demand modulation of microporosity in smart hydrogels

Aliabouzar, Mitra; Quesada, Carole; Chan, Ze Qi; Fowlkes, J. Brian; Franceschi, Renny T.; Putnam, Andrew J.; Fabiilli, Mario L.

doi:10.1016/j.actbio.2023.04.037

Cited by 4 publications

(3 citation statements)

References 59 publications

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“…We previously showed that ADV occurred at lower rarefactional pressures than IC, indicating that phase transition precedes IC [40] . However, at lower frequencies and in phase-shift droplets with higher bulk boiling points, ADV and IC could happen at similar acoustic pressures [41] . The selected P r was above the IC threshold for phase-shift droplets at 1 MHz and 2.5 MHz [40] , [42] .…”

Section: Methodsmentioning

confidence: 97%

“…The selected P r was above the IC threshold for phase-shift droplets at 1 MHz and 2.5 MHz [40] , [42] . In our prior studies IC thresholds of 1.7 ± 0.2 MPa and 5.6 ± 0.4 MPa were measured in fibrin-only hydrogels at 1.1 MHz (pulse repetition frequency (PRF): 100 Hz) and 2.5 MHz (PRF: 100 Hz), respectively [40] , [41] . However, in the current study no IC was observed in fibrin-only hydrogels due to the use of single burst ultrasound.…”

Section: Methodsmentioning

confidence: 99%

“…When accounting for the elastic behavior of the material, the gas pressure can be calculated by adding a material stress term S corresponding to the elastic behavior of the material. The form can be chosen for a suitable material model in general, and for our choice of Neo-Hookean-like elasticity, S is given by [44] :

.Following the approaches of [45] , [46] and incorporating the material stress term, we determined the relation for the quasistatic bubble radius growth rate in fibrin hydrogels to be

where D is mass diffusion coefficient ( D = 2 × 10 -9 m 2 /s for air in water),

is gas density in the bubble (1.2 kg/m 3 for air at ambient pressure),

is ambient pressure (101 kPa), G is the linear shear elastic modulus of fibrin hydrogels [41] ,

is gas concentration (kg/m 3 ) away from the bubble,

is gas concentration at the bubble surface, and γ is surface tension (0.065 N/m) [47] . The constant

was determined based on Henry’s law for oxygen and nitrogen gases in water at 37 °C at the ambient pressure and dimensionless ratio

was treated as a fitting parameter [45] .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Ultra-high-speed dynamics of acoustic droplet vaporization in soft biomaterials: Effects of viscoelasticity, frequency, and bulk boiling point

Abeid,

Fabiilli,

Estrada

et al. 2024

Ultrasonics Sonochemistry

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Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

.Following the approaches of [45] , [46] and incorporating the material stress term, we determined the relation for the quasistatic bubble radius growth rate in fibrin hydrogels to be

where D is mass diffusion coefficient ( D = 2 × 10 -9 m 2 /s for air in water),

is gas density in the bubble (1.2 kg/m 3 for air at ambient pressure),

is ambient pressure (101 kPa), G is the linear shear elastic modulus of fibrin hydrogels [41] ,

is gas concentration (kg/m 3 ) away from the bubble,

is gas concentration at the bubble surface, and γ is surface tension (0.065 N/m) [47] . The constant

was determined based on Henry’s law for oxygen and nitrogen gases in water at 37 °C at the ambient pressure and dimensionless ratio

was treated as a fitting parameter [45] .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Ultra-high-speed dynamics of acoustic droplet vaporization in soft biomaterials: Effects of viscoelasticity, frequency, and bulk boiling point

Abeid,

Fabiilli,

Estrada

et al. 2024

Ultrasonics Sonochemistry

Self Cite

View full text Add to dashboard Cite

Unlocking Transplant Tolerance with Biomaterials

Pham,

Coronel

2024

Adv Healthcare Materials

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For patients suffering from organ failure due to injury or autoimmune disease, allogeneic organ transplantation with chronic immunosuppression is considered the gold standard in terms of clinical treatment. However, the true “holy grail” of transplant immunology is operational tolerance, in which the recipient exhibits a sustained lack of alloreactivity toward unencountered antigen presented by the donor graft. This outcome is resultant from critical changes to the phenotype and genotype of the immune repertoire predicated by the activation of specific signaling pathways responsive to soluble and mechanosensitive cues. Biomaterials have emerged as a medium for interfacing with and reprogramming these endogenous pathways towards tolerance in precise, minimally invasive, and spatiotemporally defined manners. By viewing seminal and contemporary breakthroughs in transplant tolerance induction through the lens of biomaterials‐mediated immunomodulation strategies – which include intrinsic material immunogenicity, the depot effect, graft coatings, induction and delivery of tolerogenic immune cells, biomimicry of tolerogenic immune cells, and in situ reprogramming – this review emphasizes the stunning diversity of approaches in the field and spotlights exciting future directions for research to come.This article is protected by copyright. All rights reserved

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Real-time spatiotemporal characterization of mechanics and sonoporation of acoustic droplet vaporization in acoustically responsive scaffolds

Aliabouzar,

Abeid,

Kripfgans

et al. 2023

Applied Physics Letters

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Phase-shift droplets provide a flexible and dynamic platform for therapeutic and diagnostic applications of ultrasound. The spatiotemporal response of phase-shift droplets to focused ultrasound, via the mechanism termed acoustic droplet vaporization (ADV), can generate a range of bioeffects. Although ADV has been used widely in theranostic applications, ADV-induced bioeffects are understudied. Here, we integrated ultra-high-speed microscopy, confocal microscopy, and focused ultrasound for real-time visualization of ADV-induced mechanics and sonoporation in fibrin-based, tissue-mimicking hydrogels. Three monodispersed phase-shift droplets—containing perfluoropentane (PFP), perfluorohexane (PFH), or perfluorooctane (PFO)—with an average radius of ∼6 μm were studied. Fibroblasts and tracer particles, co-encapsulated within the hydrogel, were used to quantify sonoporation and mechanics resulting from ADV, respectively. The maximum radial expansion, expansion velocity, induced strain, and displacement of tracer particles were significantly higher in fibrin gels containing PFP droplets compared to PFH or PFO. Additionally, cell membrane permeabilization significantly depended on the distance between the droplet and cell (d), decreasing rapidly with increasing d. Significant membrane permeabilization occurred when d was smaller than the maximum radius of expansion. Both ultra-high-speed and confocal images indicate a hyper-local region of influence by an ADV bubble, which correlated inversely with the bulk boiling point of the phase-shift droplets. The findings provide insight into developing optimal approaches for therapeutic applications of ADV.

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Acoustic droplet vaporization for on-demand modulation of microporosity in smart hydrogels

Cited by 4 publications

References 59 publications

Ultra-high-speed dynamics of acoustic droplet vaporization in soft biomaterials: Effects of viscoelasticity, frequency, and bulk boiling point

Ultra-high-speed dynamics of acoustic droplet vaporization in soft biomaterials: Effects of viscoelasticity, frequency, and bulk boiling point

Unlocking Transplant Tolerance with Biomaterials

Real-time spatiotemporal characterization of mechanics and sonoporation of acoustic droplet vaporization in acoustically responsive scaffolds

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