Acoustic bubble for spheroid trapping, rotation, and culture: a tumor-on-a-chip platform (ABSTRACT platform)

Abstract

Cancer is the leading cause of death globally, with 90% of deaths being caused by cancer metastasis. Circulating tumor cells (CTCs) play an important role in early diagnosis of cancer...

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

“…Gao et al developed a microfluidic device with acoustic bubbles to isolate, collect, and culture spheroids with circulating tumour cells. 42 In this device, flow rates were optimized to capture the cells and form spheroids inside the bubbles. Moreover, the formed spheroids were released into the sub-channels in the devices for further culture and observation.…”

“…(A) A microfluidic platform was developed to study the cellular interaction and angiogenesis between tumour spheroids and perfused blood vessels induced by endothelial cells. 42 (B) Co-culture microfluidic platform for culturing tumour spheroids, fibroblasts, and macrophages. Behaviour of fibroblasts and macrophages co-cultured with tumour spheroids is studied within the platform.…”

“…Fig.3Interaction of tumour spheroids with other cell types within the tumour microenvironment in microfluidic platforms. (A) A microfluidic platform was developed to study the cellular interaction and angiogenesis between tumour spheroids and perfused blood vessels induced by endothelial cells 42. (B) Co-culture microfluidic platform for culturing tumour spheroids, fibroblasts, and macrophages.…”

On-demand liquid microlens arrays by non-contact relocation of inhomogeneous fluids in acoustic fields

“…After being passed into a microfluidic chip, 70 μm cell clusters were formed and fixed by the action of acoustic bubbles and UV curing. 54 The cells emitted green fluorescence under 490 nm light excitation, as shown in the bright field and fluorescence images in Fig. S5a and b, † which were taken by the microscope system (with a 4× objective lens).…”

Multifunctional Gelatin-Nanoparticle-Modified Chip for Enhanced Capture and Non-Destructive Release of Circulating Tumor Cells

“…The traditional physical methods based on cellular size, electric properties, density, and deformability may cause cell loss and low purity. In order to solve these problems, researchers have developed diverse mechanisms of CTC enrichment, such as immunomagnetic separation [ 15 , 16 ], microfluidic chips [ 17 , 18 , 19 , 20 ], and micro-filter devices [ 21 , 22 ]. Although these methods have their own advantages, the complicated experimental operations and the long analytical time limit their further application.…”