Acoustic alterations of ultrasonic vocalization in rat pups induced by perinatal hypothyroidism

Wada, Hiromi

doi:10.1016/j.neuro.2016.05.017

Cited by 6 publications

(7 citation statements)

References 52 publications

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“…Previous studies have elucidated the developmental changes in the acoustic characteristics of USV emitted by rat pups upon maternal isolation (Kehoe & Harris, 1989;Elsner et al, 1990;Brudzynski et al, 1999;Schwarting & Wohr, 2012;Kromkhun et al, 2013;Wada, 2017) and reported that the number of USVs increases on PNDs 3-5 to reach a…”

Section: Discussionmentioning

confidence: 99%

“…USVs are generated by high-pressure air flow from the thorax through a small orifice located in the closed vocal folds (Brudzynski & Fletcher, 2010). An increase in the rate of development of the size and length of the larynx and vocal tract would be expected to produce lower fundamental frequencies of USVs, which has been previously reported to occur as rat pups age (Elsner et al, 1990;Wada, 2017).…”

Section: Effects Of Ethanol On Peripheral Vocalization-related Organsmentioning

confidence: 93%

“…Where the fundamental frequency at the start and end points of USV was < 30 and ˃ 70 kHz, USV was re-analyzed with the manual selection mode. USVs that satisfied all of the following criteria were selected for statistical analyses (Reno et al, 2013;Wada, 2017). i) Fundamental frequencies at both start and end points of USV were ≥30 and <70 kHz.…”

Section: Statistical Analysesmentioning

confidence: 99%

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Effects of prenatal ethanol exposure on acoustic characteristics of ultrasonic vocalizations in rat pups

Shahrier

Wada

2018

NeuroToxicology

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Rat pups produce ultrasonic vocalizations (USVs) on isolation from their dam. Ultrasonic vocalization is a sensitive tool for evaluating social behavior between pups and their dam. Prenatal ethanol-exposure leads to a reduction in USVs and have the potential of inducing difficulties in social behavior between pups and their dam. However, effects of prenatal ethanol-exposure on the acoustic characteristics of USVs remain unclear. In this study, we recorded USVs produced by rat pups that were prenatally exposed to ethanol and examined their acoustic characteristics. Ethanol was administered to 13 pregnant rats in three stages by gradually increasing concentrations between gestational days (GDs) 8-20. From GDs 14-20, ethanol-containing tap water at concentrations of 30% and 15% (v/v) was administered to the high- and low-ethanol groups, respectively. Tap water without ethanol was given to the control group. On postnatal days (PNDs) 4, 8, 12, and 16, individual newly-born pups were isolated from their dam and littermates and USVs produced by them were recorded for 5 min. The number of USVs in the high-ethanol group was greater than that in both low-ethanol and control groups on PND 12. The mean, minimum, and maximum fundamental frequencies of USVs were elevated in the high-ethanol group compared with that in both low-ethanol and control groups. Higher amplitudes of USVs were produced by male pups in the high-ethanol group than in those in both low-ethanol and control groups on PND 12. These results suggest that prenatal ethanol exposure changed emotionality and accordingly, the high-ethanol group produced more USVs as distress calls.

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Section: Discussionmentioning

confidence: 99%

Section: Effects Of Ethanol On Peripheral Vocalization-related Organsmentioning

confidence: 93%

Section: Statistical Analysesmentioning

confidence: 99%

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Effects of prenatal ethanol exposure on acoustic characteristics of ultrasonic vocalizations in rat pups

Shahrier

Wada

2018

NeuroToxicology

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“…Mice are also born deaf, but their auditory function begins around postnatal day 10, which is when the number of USVs in produced by mice sharply decreases (Ehret, 2005;Wiaderkiewicz et al, 2013). The hypothyroid rat pups weighed significantly less than controls (Wada, 2017), though, so this may also reflect a difference in sound production from potentially underdeveloped vocal cords. Although we have not noted any differences in body weight due to BPA exposure at this dose, several studies have reported that BPA may disrupt thyroid hormone function (Chevrier et al, 2013;Sheng et al, 2012).…”

Section: Ultrasonic Vocalizationsmentioning

confidence: 87%

“…The mechanism behind the increase in median frequency and call duration in BPA pups reported in chapter two is unclear. In a recent study in rats, perinatal hypothyroidism affected the acoustic characteristics and developmental timeline of pup USVs during isolation (Wada, 2017). Production of USVs decreases after auditory function develops, but the hypothyroid pups continued vocalizing after this point, indicating an auditory developmental delay.…”

Section: Ultrasonic Vocalizationsmentioning

confidence: 91%

The Effects of Bisphenol A Exposure and Calbindin Knockout on Behavior, Gene Expression, and Sexual Differentiation of the Brain

Harris

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Bisphenol A (BPA) is an endocrine disrupting compound used in the production of polycarbonate plastics. Recent data have indicated health risks associated with exposure to BPA, particularly in children exposed during gestation. Gestational BPA exposure has been proposed as a risk factor for the development of neurobehavioral disorders, such as autism spectrum disorder. To address the behavioral impact of developmental exposure to BPA, I tested offspring of mice exposed to a daily low dose of BPA during pregnancy. I also asked if preconception exposure of the sire affected behaviors in offspring. Juvenile offspring exposed to BPA maternally, but not paternally demonstrated increased anxiety-like behavior. However, neither parental exposure group differed significantly from controls in the social recognition task. I also assessed the behaviors of maternally exposed offspring in two novel tasks: ultrasonic vocalizations (USVs) in pups and operant reversal learning in adults. Maternal BPA exposure increased the duration and median frequency of USVs emitted by pups during maternal separation. In the reversal learning task, females responded more accurately and earned more rewards than males and control females received more rewards than BPA females during the acquisition phase of the task. Previous research from our lab demonstrated that gestational BPA exposure not only affects offspring directly exposed in utero but also offspring three generations removed from the BPA exposure. We have previously reported that transgenerational exposure to BPA affects estrogen receptor alpha (ERα) in two sexually dimorphic brain areas. In a follow-up study, I determined the effects of ancestral BPA exposure in three sexually dimorphic brain regions: the anteroventral periventricular nucleus (AVPV), sexually dimorphic nucleus of the preoptic area (SDN-POA), and the cerebellum. In adults from F3 control and BPA lineages, I found that females had more tyrosine hydroxylase positive dopaminergic cells than males in the AVPV, particularly in cells that also had nuclei positive for ERα. Contrastingly, the volume of calbindin immunoreactivity in the SDN-POA was greater in males than females. In juvenile cerebellum, however, I found no effect of sex on the amount of calbindin protein. All three areas lacked a significant impact of transgenerational exposure to BPA. Calbindin, a high-affinity

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Communication and social interaction in the cannabinoid‐type 1 receptor null mouse: Implications for autism spectrum disorder

et al. 2021

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Clinical and preclinical findings have suggested a role of the endocannabinoid system (ECS) in the etiopathology of autism spectrum disorder (ASD). Previous mouse studies have investigated the role of ECS in several behavioral domains; however, none of them has performed an extensive assessment of social and communication behaviors, that is, the main core features of ASD. This study employed a mouse line lacking the primary endocannabinoid receptor (CB1r) and characterized ultrasonic communication and social interaction in CB1−/−, CB1+/−, and CB1+/+ males and females. Quantitative and qualitative alterations in ultrasonic vocalizations (USVs) were observed in CB1 null mice both during early development (i.e., between postnatal days 4 and 10), and at adulthood (i.e., at 3 months of age). Adult mutants also showed marked deficits in social interest in the three‐chamber test and social investigation in the direct social interaction test. These behavioral alterations were mostly observed in both sexes and appeared more marked in CB1−/− than CB1+/− mutant mice. Importantly, the adult USV alterations could not be attributed to differences in anxiety or sensorimotor abilities, as assessed by the elevated plus maze and auditory startle tests. Our findings demonstrate the role of CB1r in social communication and behavior, supporting the use of the CB1 full knockout mouse in preclinical research on these ASD‐relevant core domains. Lay Summary The endocannabinoid system (ECS) is important for brain development and neural function and is therefore likely to be involved in neurodevelopmental disorders such as Autism Spectrum Disorder (ASD). Here we investigated changes in social behavior and communication, which are core features of ASD, in male and female mice lacking the chief receptor of this system. Our results show that loss of this receptor results in several changes in social behavior and communication both during early development and in adulthood, thus supporting the role of the ECS in these ASD‐core behavioral domains.

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Acoustic alterations of ultrasonic vocalization in rat pups induced by perinatal hypothyroidism

Cited by 6 publications

References 52 publications

Effects of prenatal ethanol exposure on acoustic characteristics of ultrasonic vocalizations in rat pups

Effects of prenatal ethanol exposure on acoustic characteristics of ultrasonic vocalizations in rat pups

The Effects of Bisphenol A Exposure and Calbindin Knockout on Behavior, Gene Expression, and Sexual Differentiation of the Brain

Communication and social interaction in the cannabinoid‐type 1 receptor null mouse: Implications for autism spectrum disorder

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