Acknowledgements

Nissen-Meyer, R

doi:10.3109/05678066509170418

Cited by 12 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). This was not unexpected, since recombinant TNF retains its cytotoxic activity well upon dilution [5,10]. When the proportion of adherent cells was quantified by microscopy, by the MTT colorimetric assay [8], and by measurement ofthe amount of TNF they produced upon exposure to LPS, we found that the number of adherent cells after 40 min was similar to the number present after 60 min of adherence.…”

Section: Tnf Associated With Freshly Isolated Monocytes Is Produced Isupporting

confidence: 57%

Evidence that Tumour Necrosis Factor (TNF) is not Constitutively Present in Vivo The Association of TNF with Freshly Isolated Monocytes Reflects a Rapid in Vitro Production

Hofsli¹,

Lamvik²,

Nissen‐Meyer³

1988

Scand J Immunol

View full text Add to dashboard Cite

Tumour necrosis factor (TNF) cytotoxic activity has been shown not to be present in detectable amounts in the serum of healthy humans, but it may be found in some patients with meningococcal disease. In this study we investigated whether TNF is constitutively present in vivo on or within monocytes. TNF was detected on freshly isolated paraformaldehyde-fixed monocytes from both healthy individuals and cancer patients. No significant difference was found between the two groups. TNF appeared first 40-60 min after in vitro monocyte adherence, which is the same time as it took TNF to appear extracellularly after the exposure of in vitro cultured monocytes to lipopolysaccharide (LPS). This indicates that TNF associated with freshly isolated monocytes was synthesized in vitro. The inducing signal may be monocyte contact with plastic. Exposure of whole blood to LPS immediately after vein puncture was followed by about twice as rapid TNF release as that observed with in vitro cultured monocytes. The release was inhibited by cycloheximide but not by actinomycin D, indicating that the TNF detected did not represent TNF present in vivo. This is consistent with the fact that no TNF cytotoxic activity was detected in blood cell extracts. However, TNF-mRNA may have been present in vivo. Thus, the available evidence indicates that TNF is not constitutively expressed in vivo.

show abstract

Section: Tnf Associated With Freshly Isolated Monocytes Is Produced Isupporting

confidence: 57%

Evidence that Tumour Necrosis Factor (TNF) is not Constitutively Present in Vivo The Association of TNF with Freshly Isolated Monocytes Reflects a Rapid in Vitro Production

Hofsli¹,

Lamvik²,

Nissen‐Meyer³

1988

Scand J Immunol

View full text Add to dashboard Cite

show abstract

“…After further incubation of the monocyte culture for 7 h. the culture medium (monocyte supernatant) containing monocyte released TNF was rendered cellfree by low-speed centrifugation (400 x G) and stored at -20 "C. All the cytotoxic activity present in the monocyte supernatant appears to be due to TNF when using the assay system described below for measuring cytotoxicity. since neutralizing antiserum raised against rTNF completely inhibited the cytotoxic activity in the monocyte supernatants (25). Moreover, rTNF and the cytotoxic protein in monocyte supernatants had identical biological and physicochemical characteristics (25).…”

Section: Methodsmentioning

confidence: 97%

“…since neutralizing antiserum raised against rTNF completely inhibited the cytotoxic activity in the monocyte supernatants (25). Moreover, rTNF and the cytotoxic protein in monocyte supernatants had identical biological and physicochemical characteristics (25). 14.…”

Section: Methodsmentioning

confidence: 97%

“…Sect. C. 95; [21][22][23][24][25][26] 1987.The effect of different fatty acids on tumour necrosis factor (TNF)-induced cytolysis of TNF-sensitive WEHl 164 murine fibrosarcoma cells has been determined. The saturated fatty acids palmitic (16:O) and stearic ( I8:O) acid strongly potentiated the cytolytic activity of TNF.…”

mentioning

confidence: 99%

“…More recently, human TNF has been extensively characterized (I), and the gene coding for this protein has been cloned and expressed in Escherichia Cali (24, 30, 33, 35). A biological,physicochemical and immunological comparison Accepted 7.x.86 of CF and recombinant human tumour necrosis factor (rTNF) has shown that CF is identical with or very similar to rTNF (25).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Effect of Free Fatty Acids on the Cytolytic Activity of Tumour Necrosis Factor/Monocyte‐derived Cytotoxic Factor

Bjerve¹,

Espevik²,

Kildahl-Andersen³

et al. 1987

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

View full text Add to dashboard Cite

Bjerve. K.S.. Espevik. T., Kildahl-Andersen, 0. & Nissen-Meyer, J. Effect of free fatty acids on the cytolytic activity of tumour necrosis factor/monocyte-derived cytotoxic factor. Acta path. microbiol. immunol. scand. Sect. C. 95; [21][22][23][24][25][26] 1987.The effect of different fatty acids on tumour necrosis factor (TNF)-induced cytolysis of TNF-sensitive WEHl 164 murine fibrosarcoma cells has been determined. The saturated fatty acids palmitic (16:O) and stearic ( I8:O) acid strongly potentiated the cytolytic activity of TNF. They also had a slight cytotoxic effect in the absence of TNF. Contrary to this, the unsaturated fatty acids oleic (l8:1), linoleic (18:2), alpha-linolenic ( I8:3). arachidonic (20:4), and eicosapentaenoic (205) acid were neither cytotoxic by themselves, nor did they have any significant effect on the cytolytic activity of TNF. Addition of either of the unsaturated fatty acids 18:2 or 2 0 5 together with 18:O eliminated the potentiating effect 18:O by itself had on TNF-induced cytolysis. A mixture of fatty acids resembling that found in cell phospholipids had no cytotoxic effect by itself, nor any effect on the cytolytic activity of TNF. The potentiating effect of saturated fatty acids on TNF thus seems to depend on their relative amount compared to the amount of unsaturated acids. The results indicate that TNF does not exert its cytotoxic effect simply by suppressing hydrolysis of triglyceride, thereby inducing an insuficient cellular supply of fatty acids.

show abstract

Cooperative clinical trials in primary breast cancer: A critical appraisal

Fisher

1973

Cancer

View full text Add to dashboard Cite

Acknowledgements

Cited by 12 publications

References 0 publications

Evidence that Tumour Necrosis Factor (TNF) is not Constitutively Present in Vivo The Association of TNF with Freshly Isolated Monocytes Reflects a Rapid in Vitro Production

Evidence that Tumour Necrosis Factor (TNF) is not Constitutively Present in Vivo The Association of TNF with Freshly Isolated Monocytes Reflects a Rapid in Vitro Production

Effect of Free Fatty Acids on the Cytolytic Activity of Tumour Necrosis Factor/Monocyte‐derived Cytotoxic Factor

Cooperative clinical trials in primary breast cancer: A critical appraisal

Contact Info

Product

Resources

About