Acinetobacter species identification by using tRNA spacer fingerprinting

Ehrenstein, Boris; Bernards, A.T.; Dijkshoorn, Lenie; Gerner‐Smidt, Peter; Towner, K. J.; Bouvet, Philippe; Daschner, Franz; Grundmann, H.

doi:10.1128/jcm.34.10.2414-2420.1996

Cited by 103 publications

(41 citation statements)

References 26 publications

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“…1). Isolates were initially identified in the individual hospital laboratories using standard microbiological techniques, and were then confirmed as members of the A. baumannii complex [1] by tRNA fingerprinting [19].…”

Section: A T E R I a L S A N D M E T H O D S Bacterial Isolatesmentioning

confidence: 99%

OXA-51-like β-lactamases and their association with particular epidemic lineages of Acinetobacter baumannii

Evans

Hamouda

Towner

et al. 2008

Clinical Microbiology and Infection

105

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Sixty diverse clinical Acinetobacter baumannii isolates of worldwide origin were assigned to sequence groups, based on a multiplex PCR for the ompA, csuE and bla(OXA-51-like) genes. The majority (77%) of isolates belonged to sequence groups 1 and 2 (SG1 and SG2), with sequence group 3 (SG3) and non-grouped isolates accounting for the remainder. The isolates were not closely related according to pulsed-field gel electrophoresis (PFGE), and the majority were sensitive to imipenem and meropenem. The construction of a linkage map of OXA-51-like beta-lactamase sequence relationships revealed two closely related clusters of enzymes, one focused around OXA-66 and the other around OXA-69. Isolates belonging to SG1 encoded an enzyme from the OXA-66 cluster, while those belonging to SG2 encoded an enzyme from the OXA-69 cluster. All SG3 isolates encoded OXA-71, which does not form part of a close enzyme grouping. Major multinational lineages accounted for a significant proportion of A. baumannii clinical isolates, and the evolution of the OXA-51-like enzymes appears to be an ongoing process.

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Section: A T E R I a L S A N D M E T H O D S Bacterial Isolatesmentioning

confidence: 99%

OXA-51-like β-lactamases and their association with particular epidemic lineages of Acinetobacter baumannii

Evans

Hamouda

Towner

et al. 2008

Clinical Microbiology and Infection

105

View full text Add to dashboard Cite

show abstract

“…Twenty-four isolates were selected randomly for detailed retrospective study, and these were further confirmed as A. baumannii by tRNA spacer fingerprinting [20].…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Emergence and spread of carbapenem-resistant strains of Acinetobacter baumannii in a tertiary-care hospital in Poland

Wróblewska

Towner

Marchel

et al. 2007

Clinical Microbiology and Infection

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This study analysed the occurrence of carbapenem resistance among Acinetobacter baumannii isolates from a tertiary-care hospital in Poland, together with the molecular epidemiology of these isolates and the risk-factors for their acquisition and possible nosocomial spread. The medical charts of 21 patients with Acinetobacter infection or colonisation revealed that A. baumannii isolates were obtained most frequently from intensive care unit and surgical patients (particularly those receiving transplantation surgery). First isolation occurred, on average, on day 21 following admission (range 5-45 days). Infection with Acinetobacter contributed directly to the death of seven patients. Several patients were infected with more than one strain, and molecular typing revealed the co-circulation of three predominant clones, of which two belonged to the Acinetobacter lineages designated as European clones I and II. All three clones encoded an OXA-51-type carbapenemase, but were negative for carbapenemases belonging to the OXA-23, OXA-24 and OXA-58 families. The OXA-51 gene was found in both resistant and susceptible isolates, and was not associated directly with carbapenem resistance. Etests with imipenem and imipenem plus EDTA indicated production of a metallo-beta-lactamase (MBL) in carbapenem-resistant isolates. PCRs for IMP-type MBLs were negative, but PCR using consensus primers for VIM-type MBLs were positive for carbapenem-resistant isolates belonging to the European clone II lineage. The occurrence of a VIM-type MBL in association with one of the epidemic lineages of A. baumannii is a cause for concern. Further studies are needed to evaluate possible inter-hospital spread of resistant A. baumannii strains in Poland.

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“…baumannii species (Dijkshoorn et al 2007). A breakthrough in the identification of Acinetobacter species was achieved with the development of advanced molecular methods such as amplified 16S rRNA gene restriction analysis (ARDRA) (Vaneechoutte et al 1995), amplified fragment length polymorphism (AFLP) (Janssen et al 1997), ribotyping (Gerner-Smidt 1992), tRNA spacer fingerprinting (Ehrenstein et al 1996), restriction analysis of the 16S-23S rRNA intergenic spacer sequences (ITS) (Dolzani et al 1995) and sequence analysis of the 16S-23S rRNA gene spacer region (Chang et al 2005). Although molecular methods have been proven as reliable and sensitive techniques for species identification, but the obstacles associated with these techniques (e.g., high cost, being laborious, need for complicated instrument and welltrained personal) restricted their dissemination in routine laboratory identification of Acinetobacter.…”

Section: Introductionmentioning

confidence: 99%

A sensitive colorimetric assay for identification of Acinetobacter baumannii using unmodified gold nanoparticles

et al. 2014

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Aims: Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). Methods and Results: Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals. Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction using boiling method and PCR amplification of the 23S-16S rRNA intergenic spacer sequences (ITS). AuNPs were synthesized using citrate reduction method. Detection and optimization of Ac. baumannii amplicons using unmodified spherical AuNPs were performed using species-specific DNA oligonucleotide. The nano-gold assay was able to colorimetrically detect and distinguish Ac. baumannii from other Gram-negative bacteria. The turnaround time of the assay is about 2 h including sample treatment and amplification. The assay detection limit is 0Á8125 ng of DNA. Conclusions: The developed colorimetric assay is sensitive, fast and reliable and can be used for identification of Ac. baumannii. Significance and Impact of the Study: There is a need to develop robust, rapid, and specific methods for detection of Ac. baumannii isolated from clinical specimens. The developed nanogold assay prototype allows sensitive, specific and rapid detection of amplified DNA of A. baumannii and represents a reliable diagnostic tool to aid routine laboratory identification of this pathogen.

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Acinetobacter species identification by using tRNA spacer fingerprinting

Cited by 103 publications

References 26 publications

OXA-51-like β-lactamases and their association with particular epidemic lineages of Acinetobacter baumannii

OXA-51-like β-lactamases and their association with particular epidemic lineages of Acinetobacter baumannii

Emergence and spread of carbapenem-resistant strains of Acinetobacter baumannii in a tertiary-care hospital in Poland

A sensitive colorimetric assay for identification of Acinetobacter baumannii using unmodified gold nanoparticles

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