Acinetobacter baumannii Sampled from Cattle and Pigs Represent Novel Clones

Mateo-Estrada, Valeria; Vali, Leila; Hamouda, Ahmed; Evans, Benjamin A.; Castillo-Ramírez, Santiago

doi:10.1128/spectrum.01289-22

Cited by 12 publications

(15 citation statements)

References 24 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supporting this scenario, a 2022 genomic epidemiology paper has shown that cattle and pig isolates belong to novel clones that are not found in humans. 4 Furthermore, some other animals (eg, donkey, goat, and goose) have clones of A baumannii that have not been described in clinical settings. 3 , 5 Another theory could be that animal and human A baumannii populations are not differentiated from one another and exhibit frequent gene flow between both populations.…”

mentioning

confidence: 99%

Zoonotic Acinetobacter baumannii: the need for genomic epidemiology in a One Health context

Castillo-Ramírez

2022

The Lancet Microbe

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 99%

Zoonotic Acinetobacter baumannii: the need for genomic epidemiology in a One Health context

Castillo-Ramírez

2022

The Lancet Microbe

Self Cite

View full text Add to dashboard Cite

“…However, our results indicate that these interventions can also have negative consequences, since the dysbiosis produced by these drugs could facilitate pathogen colonization, with the consequent higher risk of infection. In fact, we detected in piglets from treated sows some potentials pathogens such as Acinetobacter [72,73], Clostridium [74] or Treponema [75] that were not found in the farm samples. In good health status farms, the colonization of these pathogens is probably controlled by the exclusion provided by the normal nasal microbiota.…”

Section: Discussionmentioning

confidence: 80%

Intensive antibiotic treatment of sows with parenteral crystalline ceftiofur and tulathromycin alters the composition of the nasal microbiota of their offspring

Bonillo-Lopez,

Obregon-Gutierrez,

Huerta

et al. 2023

Preprint

View full text Add to dashboard Cite

Nasal microbiota plays an important role in animal health and the use of antibiotics is one of the major factors that influence its composition. Here, we study the consequences on piglets’ nasal microbiota when an intensive antibiotic treatment was applied to sows and/or their piglets. Four pregnant sows received a treatment of ceftiofur and tulathromycin (CTsows) while two other sows received only crystalline ceftiofur (Csows). Sow treatments were performed at D-4, D3 and D10 for ceftiofur and D-3, D4 and D11 for tulathromycin. Half of the piglets born to CTsows were treated at birth with ceftiofur. Nasal swabs were taken from piglets at 22-24 days of age and bacterial load and nasal microbiota composition were defined by 16s rRNA gene qPCR and amplicon sequencing. Antibiotic treatment of sows reduced their nasal bacterial load, as well as in their offspring, indicating a reduced bacterial transmission from the dams. In addition, nasal microbiota composition of the piglets exhibited signs of dysbiosis, showing unusual taxa. The addition of tulathromycin to the ceftiofur treatment seemed to enhance the effect on the microbiota diversity by diminishing some bacteria commonly found in the piglets’ nasal cavity, such as Glaesserella, Streptococcus, Prevotella, Staphylococcus and several members of the Ruminococcaceae and Lachnospiraceae families. The additional treatment of piglets with ceftiofur resulted in no further effect beyond the treatment of the sows. Altogether these results suggest that intensive antibiotic treatments in sows, especially the double antibiotic treatment, disrupt the nasal microbiota of their offspring and highlight the importance of sow-to-piglet microbiota transmission.

show abstract

“…A. baumannii has been mainly studied as a hospital-associated organism since its emergence as a nosocomial pathogen in the 1970s. Nonetheless, over the last two decades, studies have shown that these bacteria can be isolated from non-clinical settings [4][5][6][7][8][9]. Yet, many of these studies have focused on animal or animal-related products and a lack of effort is evident in plant and soil environments.…”

Section: Discussionmentioning

confidence: 99%

“…A very recent study even found A. baumannii in a milk tank [11]. Clearly, isolates of this pathogen have been found in different types of environments [4,6,7,9,12]. However, to the best of our knowledge, soil and related plant environments have received little attention.…”

Section: Introductionmentioning

confidence: 99%

“…Here, employing genome sequencing and antimicrobial susceptibility tests we analyse 61 isolates sampled from a field trial in Ireland collected for 18 weeks in the summer of 2019. To provide a better framework, in our phylogenomic approach we included some recently described novel animal lineages and representative genomes from the major international clones (ICs) circulating in the clinic [6]. Our results show that grass isolates form novel clones not related to human and animal clones and with limited antibiotic resistance potential.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acinetobacter baumannii from grass: novel but non-resistant clones

et al. 2023

Self Cite

View full text Add to dashboard Cite

Acinetobacter baumannii is one the most worrisome nosocomial pathogens, which has long been considered almost mainly as a hospital-associated bacterium. There have been some studies about animal and environmental isolates over the last decade. However, little effort has been made to determine if this pathogen dwells in the grass. Here, we aim to determine the evolutionary relationships and antibiotic resistance of clones of A. baumannii sampled from grass to the major human international clones and animal clones. Two hundred and forty genomes were considered in total from four different sources for this study. Our core and accessory genomic epidemiology analyses showed that grass isolates cluster in seven groups well differentiated from one another and from the major human and animal isolates. Furthermore, we found new sequence types under both multilocus sequence typing schemes: two under the Pasteur scheme and seven for the Oxford scheme. The grass isolates contained fewer antibiotic-resistance genes and were not resistant to the antibiotics tested. Our results demonstrate that these novel clones appear to have limited antibiotic resistance potential. Given our findings, we propose that genomic epidemiology and surveillance of A. baumannii should go beyond the hospital settings and consider the environment in an explicit One Health approach.

show abstract

Acinetobacter baumannii Sampled from Cattle and Pigs Represent Novel Clones

Abstract: Over the last decades, huge amounts of information have been obtained for clinical isolates of A. baumannii and the clones they belong to. In contrast, very little is known about the genomic identity and the genomic basis for virulence and resistance of animal isolates.

Cited by 12 publications

References 24 publications

Zoonotic Acinetobacter baumannii: the need for genomic epidemiology in a One Health context

Zoonotic Acinetobacter baumannii: the need for genomic epidemiology in a One Health context

Intensive antibiotic treatment of sows with parenteral crystalline ceftiofur and tulathromycin alters the composition of the nasal microbiota of their offspring

Acinetobacter baumannii from grass: novel but non-resistant clones

Contact Info

Product

Resources

About