Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles

Moon, Dong Chan; Choi, Chul Hee; Lee, Jung Hwa; Choi, Chang Soo; Kim, Hye-Yeon; Park, Jeong Soon; Kim, Kyung Sik; Lee, Je Chul

doi:10.1007/s12275-012-1589-4

Cited by 107 publications

(87 citation statements)

References 43 publications

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“…It appears that OmpA-like domain links the outer membrane to the peptidoglycan layer and is essential for the integrity of the cell. Previous quantitative and qualitative approaches have shown that outer membrane vesicles (OMVs) production is significantly increased in the ΔAbOmpA mutant compared to the wild-type bacteria [34]. This data confirms that OmpA-like domain of AbOmpA plays an important role in the integrity of the outer membrane which may give rise to a controlled shedding of OMV from the surface of the bacteria.…”

Section: Discussionsupporting

confidence: 76%

Molecular analysis of AbOmpA type-1 as immunogenic target for therapeutic interventions against MDR Acinetobacter baumannii infection

Badmasti

Siadat

Bouzari

et al. 2015

vacres

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Introduction: Acinetobacter baumannii is associated with hospital-acquired infections. Outer membrane protein A of A.baumannii (AbOmpA) is a well-characterized virulence factor which has important roles in pathogenesis of this bacterium. Methods: Based on our PCR-sequencing of ompA gene in the clinical isolates, AbOmpA protein can be categorized into two types, named here type-1 and type-2. We are performed in silico analyses on beta-barrel domain of AbOmpA such as sequence alignments, prediction of 3D modeling and immunoinformatics. Results: High prevalence of AbOmpA type-1 were detected both in 21 multidrug-resistant (MDR) A. baumannii isolates collected from clinical settings (64% of total sequences) and in silico sequences extracted from Uniprot database (49.7% of total sequences). Multiple sequence alignments and phylogenic tree revealed AbOmpA sequences in comparison with OmpA of Enterobacteriaceae family were more heterogenic, especially at extracellular loops amino acid positions and were evolutionary far from this family. Characterization of in silico 3D molecular model of beta-barrel domain of AbOmpA type-1 showed this domain had four large extracellular loops which resemble to beta-barrel domain of Klebsiella pneumonia OmpA (KpOmpA). Immunoinformatics analyses showed the four extracellular loops had high scores as B and T cell antigenic epitopes especially in loop-1 and 3. Geometry analyses revealed extracellular loops effectively protrude to outer space of the cell. Conclusion: Beta-barrel domain of AbOmpA type-1 has all the characteristics of a promising vaccine and could be considered as an excellent target for a vaccine against MDR A. baumannii infection.

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Section: Discussionsupporting

confidence: 76%

Molecular analysis of AbOmpA type-1 as immunogenic target for therapeutic interventions against MDR Acinetobacter baumannii infection

Badmasti

Siadat

Bouzari

et al. 2015

vacres

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“…2C). OmpA is an outer membrane protein that is commonly found in Acinetobacter species (59), and IEM with anti-OmpA antibodies attached to gold particles confirmed the presence of OmpA in the OMVs (Fig. 2B).…”

Section: Discussionmentioning

confidence: 74%

Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation

Fulsundar

Harms

Flaten

et al. 2014

Appl Environ Microbiol

197

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dOuter membrane vesicles (OMVs) are continually released from a range of bacterial species. Numerous functions of OMVs, including the facilitation of horizontal gene transfer (HGT) processes, have been proposed. In this study, we investigated whether OMVs contribute to the transfer of plasmids between bacterial cells and species using Gram-negative Acinetobacter baylyi as a model system. OMVs were extracted from bacterial cultures and tested for the ability to vector gene transfer into populations of Escherichia coli and A. baylyi, including naturally transformation-deficient mutants of A. baylyi. Anti-double-stranded DNA (anti-dsDNA) antibodies were used to determine the movement of DNA into OMVs. We also determined how stress affected the level of vesiculation and the amount of DNA in vesicles. OMVs were further characterized by measuring particle size distribution (PSD) and zeta potential. Transmission electron microscopy (TEM) and immunogold labeling were performed using antifluorescein isothiocyanate (anti-FITC)-conjugated antibodies and anti-dsDNA antibodies to track the movement of FITC-labeled and DNA-containing OMVs. Exposure to OMVs isolated from plasmid-containing donor cells resulted in HGT to A. baylyi and E. coli at transfer frequencies ranging from 10 ؊6 to 10 ؊8 , with transfer efficiencies of approximately 10 3 and 10 2 per g of vesicular DNA, respectively. Antibiotic stress was shown to affect the DNA content of OMVs as well as their hydrodynamic diameter and zeta potential. Morphological observations suggest that OMVs from A. baylyi interact with recipient cells in different ways, depending on the recipient species. Interestingly, the PSD measurements suggest that distinct size ranges of OMVs are released from A. baylyi.

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“…and Acinetobacter baumannii mutants lacking OmpA display increased OMV production 34–37 , most probably as a consequence of decreased crosslinking between PG and the outer membrane 30 . Interestingly, although modulation of this type of envelope crosslink also seemed to lead to hypervesiculation in Pseudomonas aeruginosa mutants lacking OprF (an OmpA homologue 38 ), in this case hypervesiculation is actually a consequence of an increase in the levels of Pseudomonas quinolone signal (PQS), which stimulates OMV production in P. aeruginosa 39 (see below).…”

Section: Omv Biogenesismentioning

confidence: 99%

Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions

2015

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Outer-membrane vesicles (OMVs) are spherical buds of the outer membrane filled with periplasmic content and are commonly produced by Gram-negative bacteria. The production of OMVs allows bacteria to interact with their environment, and OMVs have been found to mediate diverse functions, including promoting pathogenesis, enabling bacterial survival during stress conditions and regulating microbial interactions within bacterial communities. Additionally, because of this functional versatility, researchers have begun to explore OMVs as a platform for bioengineering applications. In this Review, we discuss recent advances in the study of OMVs, focusing on new insights into the mechanisms of biogenesis and the functions of these vesicles.

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Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles

Cited by 107 publications

References 43 publications

Molecular analysis of AbOmpA type-1 as immunogenic target for therapeutic interventions against MDR Acinetobacter baumannii infection

Molecular analysis of AbOmpA type-1 as immunogenic target for therapeutic interventions against MDR Acinetobacter baumannii infection

Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation

Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions

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