Acinetobacter apis sp. nov., isolated from the intestinal tract of a honey bee, Apis mellifera

Abstract

A novel Gram-negative, obligate aerobic, non-motile, and both coccobacillus- and bacillus-shaped bacterium, designated strain HYN18(T), was isolated from the intestinal tract of a honey bee (Apis mellifera). The isolate was oxidase-negative and catalase-positive. Strain HYN18(T) showed optimum growth at 25°C, pH 6-7, and in the presence of 1% (w/v) NaCl in trypticase soy broth medium. The isolate was negative for hydrolyses of starch, casein, gelatin and urea, indole production from tryptone and hemolysis on sheep blood agar. A phylogenetic analysis based on the 16S rRNA gene and rpoB gene sequence showed that strain HYN18(T) was most closely related to Acinetobacter nectaris SAP 763.2(T) and A. boissieri SAP 284.1(T) with 98.3% and 98.1% similarity (16S rRNA gene), respectively, and 84.4% similarity with Acinetobacter nectaris SAP 763.2(T) (rpoB gene). The major cellular fatty acids were summed features 3 (comprising C16:1ω7c /C16:1ω6c ), C12:0 and C16:0. The main isoprenoid quinone was ubiquinone-9 (Q-9). The polar lipids of strain HYN18(T) were phosphatidylethanolamine, three unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 40.6 mol%. DNA-DNA hybridization experiments indicated less than 33 ± 10% relatedness to the closest phylogenetic species, Acinetobacter nectaris SAP 763.2(T). Thus, the phenotypic, phylogenetic and genotypic analyses indicate that strain HYN18(T) is a novel species within the genus Acinetobacter, for which the name Acinetobacter apis is proposed. The type strain is HYN18(T) (=KACC 16906(T) =JCM 18575(T)).

Different Dynamics of Bacterial and Fungal Communities in Hive-Stored Bee Bread and Their Possible Roles: A Case Study from Two Commercial Honey Bees in China

“…Two species of Acinetobacter have been found as novel species in the nectar of a wild plant in the Mediterranean: Acinetobacter nactaris and Acinetobacter boissieri [86]. Furthermore, Acinetobacter apis has been discovered in the intestinal tract of A. mellifera [87]. Many studies have shown that Acinetobacter species thrive in a sugar-rich habitat and have even formed a symbiosis with the Pae aphid, a phloem sap-eating insect [88].…”

Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864)

“…The genus Acinetobacter is currently comprised of fifty-five distinct species that have been originally isolated from various sources, such as human clinical specimens, invertebrate and vertebrate animals, vegetables, soil and water [34,35]. Although research on the genus Acinetobacter has been particularly focused on some species that have effects on human health, especially A. baumannii, the beneficial effects of most of the remaining species on their hosts have never been addressed to date.…”

Ventosimonas gracilis gen. nov., sp. nov., a member of the Gammaproteobacteria isolated from Cephalotes varians ant guts representing a new family, Ventosimonadaceae fam. nov., within the order ‘Pseudomonadales’

“…The only respiratory quinone detected is ubiquinone-9 (Q-9) and Table 3. Phenotypic Characteristics of Ventosimonas gracilis CV58 T compared to other cultivated representatives from the order Pseudomonadales Strains: 1, Ventosimonas gracilis CV58 T ; 2, Pseudomonas aeruginosa ATCC 27853 (G+C content from Fang et al, 2012); 3, Pseudomonas arsenicoxydans VC-1 T (data from Campos et al, 2010); 4, Acinetobacter apis HYN18 T (data from Kim et al, 2014). Substrate usage and enzyme activity were determined using GN2 and GEN III microplates and API 20 NE strips.…”