Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges

Abstract

A Gram-stain-negative, rod-shaped, aerobic, straw yellow, motile strain, designated KNDSW-TSA6, belonging to the genus Acidovorax, was isolated from a water sample of the river Ganges, downstream of the city of Kanpur, Uttar Pradesh, India. Cells were aerobic, non-endospore-forming and motile with single polar flagella. It differed from its phylogenetically related strains by phenotypic characteristics such as hydrolysis of urea, gelatin, casein and DNA, and the catalase reaction. The major fatty acids were C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1ω7c/C18 : 1ω6c. Phylogenetic analysis based on 16S rRNA and housekeeping genes (gyrb, recA and rpoB gene sequences), confirmed its placement within the genus Acidovorax as a novel species. Strain KNDSW-TSA6 showed highest 16S rRNA sequence similarity to Acidovorax soli BL21 (98.9 %), Acidovorax delafieldii ATCC 17505 (98.8 %), Acidovorax temperans CCUG 11779 (98.2 %), Acidovorax caeni R-24608 (97.9 %) and Acidovorax radicis N35 (97.6 %). The digital DNA-DNA hybridization and average nucleotide identity values calculated from whole genome sequences between strain KNDSW-TSA6 and the two most closely related strains A. soli BL21 and A. delafieldii ATCC 17505 were below the threshold values of 70 and 95 % respectively. Thus, the data from the polyphasic taxonomic analysis clearly indicates that strain KNDSW-TSA6 represents a novel species, for which the name Acidovorax kalamii sp. nov. is proposed. The type strain is Acidovorax kalamii (=MTCC 12652=KCTC 52819=VTCC-B-910010).

Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community

“…Finally, members of the Acidovorax genus were over-represented in the adductor muscle and digestive gland bacterial communities. Members of the Acidovorax have been isolated from a diverse range of environments including soil (Chaudhary & Kim, 2018), water (Pal et al, 2018), and from cyanobacterial blooms (Chun et al, 2017).…”

Draft Genome of Acidovorax kalamii Strain JM16, Isolated from Skin Mucus of Zebrafish (Danio rerio)

“…Members of this bacterial genus have been classified as environmental microbes, endosymbionts of earthworms, phytopathogens, and opportunistic human pathogens ( 1 – 9 ). A. kalamii was first described in 2018 ( 10 ) and cultured from a water sample of the River Ganges in India, yet relationships or ecosystem roles are undescribed.…”

Bacterial Biodiversity in Mineromedicinal Waters of Spa “Urauco”. Pichincha. Ecuador

“…En relación con la especie de bacterias Gram negativas de los géneros Acidovorax y Brevundimonas perteneciente a las alfa proteobacterias y beta proteobacterias, respectivamente, las cuales se aislaron también de las aguas termales del balneario Urauco, se pude indicar que las mismas se han aislado de otros manantiales de aguas minerales termales, tanto de Ecuador, España y Venezuela (29,37,42,50).…”