Acidogenic growth model of embryogenic cell suspension culture and qualitative mass production of somatic embryos from triploid bananas

Chung, Jen-Ping; Lu, Cheng‐Chang; Kuo, Lan-Ting; Ma, Shen; Shii, Chou-Tou

doi:10.1007/s11240-015-0888-y

Cited by 9 publications

(9 citation statements)

References 33 publications

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“…The moment when the pH is increasing again as said to coincide with the first embryo formation ( de Feria et al, 2003 ; Barbón et al, 2008 ). Changes in pH seems to be an important factor in the regulation of SE and can be controlled naturally by the growth and normal substances release from the cells ( Chung et al, 2006 , 2016 ). The cell density also influences SE.…”

Section: Coffee Somatic Embryogenesismentioning

confidence: 99%

Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Campos

Panis²,

Carpentier

2017

Front. Plant Sci.

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One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied in order to provide practical results for coffee improvement. While genetic transformation got many attention in the past and is booming with the CRISPR technology, micropropagation and SE are still the major bottle neck and urgently need more attention. The methodologies to induce SE and the further development of the embryos are genotype-dependent, what leads to an almost empirical development of specific protocols for each cultivar or clone. This is a serious limitation and excludes a general comprehensive understanding of the process as a whole. The aim of this review is to provide an overview of which achievements and molecular insights have been gained in (coffee) somatic embryogenesis and encourage researchers to invest further in the in vitro technology and combine it with the latest omics techniques (genomics, transcriptomics, proteomics, metabolomics, and phenomics). We conclude that the evolution of biotechnology and the integration of omics technologies offer great opportunities to (i) optimize the production process of SE and the subsequent conversion into rooted plantlets and (ii) to screen for possible somaclonal variation. However, currently the usage of the latest biotechnology did not pass the stage beyond proof of potential and needs to further improve.

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Section: Coffee Somatic Embryogenesismentioning

confidence: 99%

Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Campos

Panis²,

Carpentier

2017

Front. Plant Sci.

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“…Maturation, the final stage of embryo development is distinguished by overall cell expansion and accumulation of reserve substances of the embryo. In this regard, the quality and quantity of banana somatic embryos have shown to be influenced by medium pH, the so-called ECS acidogenic growth hypothesis (Chung et al 2015). In several edible bananas genotypes, the basis for the low quality and quantity of the somatic embryos, and the poor regeneration and conversion to vigorous plants is frequently the least studied aspects in banana (Krikorian and Scott 1995).…”

Section: Development and Maturation Of Somatic Embryomentioning

confidence: 99%

“…The addition of the amino acids L-glutamine, alanine or L-glutamic acid have shown to strongly stimulate the development of somatic embryos in carrot (Higashi et al 1996). 21.1g) around 45 days of culture (Côte et al 1996;Chung et al 2015), as is in other plant species (Márquez-Martín et al 2011). Berangan) regeneration (Husin et al 2014).…”

Section: Development and Maturation Of Somatic Embryomentioning

confidence: 99%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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“…In several edible bananas genotypes, the basis for the low quality and quantity of the somatic embryos, and the poor regeneration and conversion to vigorous plants is frequently the least studied aspects in banana (Krikorian and Scott 1995). In this regard, the quality and quantity of banana somatic embryos have shown to be influenced by medium pH, the so-called ECS acidogenic growth hypothesis (Chung et al 2015). Five types of cell aggregates has been recognized in banana ECS cultures of Musa AAA cv.…”

Section: Development and Maturation Of Somatic Embryomentioning

confidence: 99%

“…Available banana protocols control the water availability to the developing somatic embryos by using either higher concentration of gelling gum and/or filter paper or both (Table 21.3), under this circumstances accumulation of starch in during embryo maturity is indicated by the white-opaque color (Fig. 21.1g) around 45 days of culture (Côte et al 1996;Chung et al 2015), as is in other plant species (Márquez-Martín et al 2011). Molecular evidences from transcribed expressed sequences have suggested that an early responsive to dehydration proteins (ERDs) are involved in embryo maturation.…”

Section: Development and Maturation Of Somatic Embryomentioning

confidence: 99%

Somatic Embryogenesis in Banana, Musa ssp.

Escobedo-GraciaMedrano

Enríquez-Valencia

Youssef

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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In Musa (Musaceae family), as for other angiosperms, somatic embryo formation from somatic cells exemplify a distinctive phenomenon of plant cell developmental plasticity. Somatic embryogenesis (SE) through embryogenic cell suspension (ECS) cultures is an important milestone method for accelerating bananas' mass-propagation due to its high regeneration potential, and serves as powerful cellular tool for its non-conventional improvement. Protocols for SE have been standardized for several genotypes of wild Musa species (having AA and/or BB genomes), dessert (AA, AB and AAA), cooking (ABB), and plantain (AAB) bananas using different types of explants; however, in some cases, the protocols are limited by the low embryo germination and plant conversion rates. Therefore, efforts are needed to understand the physiological, biochemical, and genetic processes underpinning banana embryo development (zygotic and somatic), in order to inaugurate robust SE protocols with high rates of embryo germination and plant conversion. Here, we present an overview of the general progress in banana plant regeneration through somatic embryogenesis. Background InformationBananas and plantains, collectively known as bananas, belong to the genus Musa (Family: Musaceae); these monocotyledonous giant herbs are among the most important horticultural crops widely distributed throughout the humid tropical and

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Acidogenic growth model of embryogenic cell suspension culture and qualitative mass production of somatic embryos from triploid bananas

Cited by 9 publications

References 33 publications

Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities

Somatic Embryogenesis: Fundamental Aspects and Applications

Somatic Embryogenesis in Banana, Musa ssp.

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