Acidithiobacillus ferriphilus sp. nov., a facultatively anaerobic iron- and sulfur-metabolizing extreme acidophile

Falagán, Carmen; Johnson, D. Barrie

doi:10.1099/ijsem.0.000698

Cited by 69 publications

(47 citation statements)

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“…Based on a careful re-evaluation of phenotypic characteristics (e.g., capacity to oxidize molecular hydrogen, temperature, and pH tolerance profiles, tolerance to elevated concentrations of transition metals and chloride, presence of flagella, etc.) and multilocus sequence analyses, three novel iron-oxidizing species have been recently recognized, Acidithiobacillus ferrivorans (Hallberg et al, 2010), Acidithiobacillus ferridurans (Hedrich and Johnson, 2013), and Acidithiobacillus ferriphilus (Falagán and Johnson, 2016), enlarging the genus to a current total of seven species. Provisional recognition of a number of additional ( Acidi ) thiobacillus species—associated to particular niches—has occurred in the past, e.g., “( Acidi)thiobacilus concretivorus,” the predominant isolate during the acidification associated to the final stage of concrete corrosion (Parker, 1945a,b, 1947) and “ Acidithiobacillus cuprithermicus,” described as a novel isolate growing on chalcopyrite obtained from the Tinto River (Fernández-Remolar et al, 2003), though the validity of some proposed novel “species” has often been questioned (e.g., Vishniac and Santer, 1957).…”

Section: Introductionmentioning

confidence: 99%

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

et al. 2017

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The acidithiobacilli are sulfur-oxidizing acidophilic bacteria that thrive in both natural and anthropogenic low pH environments. They contribute to processes that lead to the generation of acid rock drainage in several different geoclimatic contexts, and their properties have long been harnessed for the biotechnological processing of minerals. Presently, the genus is composed of seven validated species, described between 1922 and 2015: Acidithiobacillus thiooxidans, A. ferrooxidans, A. albertensis, A. caldus, A. ferrivorans, A. ferridurans, and A. ferriphilus. However, a large number of Acidithiobacillus strains and sequence clones have been obtained from a variety of ecological niches over the years, and many isolates are thought to vary in phenotypic properties and cognate genetic traits. Moreover, many isolates remain unclassified and several conflicting specific assignments muddle the picture from an evolutionary standpoint. Here we revise the phylogenetic relationships within this species complex and determine the phylogenetic species boundaries using three different typing approaches with varying degrees of resolution: 16S rRNA gene-based ribotyping, oligotyping, and multi-locus sequencing analysis (MLSA). To this end, the 580 16S rRNA gene sequences affiliated to the Acidithiobacillus spp. were collected from public and private databases and subjected to a comprehensive phylogenetic analysis. Oligotyping was used to profile high-entropy nucleotide positions and resolve meaningful differences between closely related strains at the 16S rRNA gene level. Due to its greater discriminatory power, MLSA was used as a proxy for genome-wide divergence in a smaller but representative set of strains. Results obtained indicate that there is still considerable unexplored diversity within this genus. At least six new lineages or phylotypes, supported by the different methods used herein, are evident within the Acidithiobacillus species complex. Although the diagnostic characteristics of these subgroups of strains are as yet unresolved, correlations to specific metadata hint to the mechanisms behind econiche-driven divergence of some of the species/phylotypes identified. The emerging phylogenetic structure for the genus outlined in this study can be used to guide isolate selection for future population genomics and evolutionary studies in this important acidophile model.

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Section: Introductionmentioning

confidence: 99%

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

et al. 2017

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“…The most extensively studied taxa of bacteria in previous studies are chemoautotrophic Acidithiobacillus spp. and Leptospirillum spp., which are generally recognized as the ringleader of the AMD problem [38,39]. Sulfur-oxidizing Thiobacillus spp.…”

Section: Amd Generation and Associated Microorganismsmentioning

confidence: 99%

Microbial Diversity and Community Assembly across Environmental Gradients in Acid Mine Drainage

et al. 2017

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Abstract:Microorganisms play an important role in weathering sulfide minerals worldwide and thrive in metal-rich and extremely acidic environments in acid mine drainage (AMD). Advanced molecular methods provide in-depth information on the microbial diversity and community dynamics in the AMD-generating environment. Although the diversity is relatively low and in general inversely correlated with the acidity, a considerable number of microbial species have been detected and described in AMD ecosystems. The acidophilic microbial communities dominated by iron/sulfur-oxidizing microbes vary widely in their composition and structure across diverse environmental gradients. Environmental conditions affect the microbial community assembly via direct and indirect interactions with microbes, resulting in an environmentally dependent biogeographic pattern. This article summarizes the latest studies to provide a better understanding of the microbial biodiversity and community assembly in AMD environments.

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“…Therefore, several studies reported At. ferrooxidans from acidic environments . As their natural habitats are ecologically enormously diverse and different strains of the same species are thriving in many ecological niches that are characterized by dissimilar growth rate, activities of ferrous and sulfide minerals oxidation, and tolerance toward heavy metals.…”

Section: Discussionmentioning

confidence: 99%

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Sajjad

Zheng

et al. 2018

J Basic Microbiol

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The exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous ironoxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope, China. The morphological, biochemical, physiological, and phylogenetic characteristics of isolates were investigated. These isolates were motile, Gram-negative, and curved shape with pleomorphism except isolate WG101 that was a straight rod. The optimum growth pH and temperature for all isolates were 1.5 and 30°C, respectively, and showed extreme acidophilic nature. All the isolates showed obligate chemoautotrophic nature and used ferrous iron and pyrite as an energy source, however, isolates WG102 and WG103 were unable to use sulfur, while isolate WG101 could use elemental sulfur and reduced inorganic sodium thiosulfate as an energy source. The phylogenetic analyses based on 16S rRNA sequences revealed that the isolates WG101, WG102, and WG103 were homologous with Acidithiobacillus ferrooxidans strain AS2 (99%), Leptospirillum ferriphilum strain YSK (98%), and Leptospirillum ferrooxidans strain L15 (98%), respectively. These bacterial isolates showed efficient copper and zinc dissolution from the ore. The metals dissolution rate of At. ferrooxidans strain WG101 was 54.5 ± 4.33% (copper) and 49.6 ± 5% (zinc). The metals recovery rate of L.ferriphilum strain WG102 was 45.7 ± 3.5% (copper) and 40.5 ± 2.5% (zinc). The recovery rate of copper and zinc was 49.6 ± 4% and 46.5 ± 3% respectively in the case of L. ferrooxidans strain WG103. The findings of this study are consistent with the notion that the indigenous bacteria are more efficient in minerals dissolution. K E Y W O R D SAcidithiobacillus ferrooxidans, Baiyin Copper Mine, bioleaching, Leptospirillum ferriphilum, Leptospirillum ferrooxidansAbbreviations: AMD, acid mine drainage; EPS, exopolysaccharide; ORP, oxidation-reduction potential.

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Acidithiobacillus ferriphilus sp. nov., a facultatively anaerobic iron- and sulfur-metabolizing extreme acidophile

Abstract: Acidithiobacillus ferriphilus sp. nov., a facultatively anaerobic iron-and sulfur-metabolizing extreme acidophile

Cited by 69 publications

References 15 publications

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

Microbial Diversity and Community Assembly across Environmental Gradients in Acid Mine Drainage

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

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