Acidic α-galactosidase is the most abundant nectarin in floral nectar of common tobacco (Nicotiana tabacum)

Zha, Hong-Guang; Flowers, V. Lynn; Yang, Min; Chen, Lingyang; Sun, Hang

doi:10.1093/aob/mcr321

Cited by 24 publications

(15 citation statements)

References 51 publications

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“…[13,63] as in our study. Evaluating various Nicotiana taxa, N. rustica had higher nectar protein concentrations, compared to either the other two species involved in our study (Table 1), or to Nicotiana species investigated previously [13,23,63]. The 5 major proteins in the floral nectar of the ornamental tobacco N. langsdorffii × N. sanderae var.…”

Section: Alkaloid Content Of the Floral Nectarmentioning

confidence: 57%

Protein and alkaloid patterns of the floral nectar in some solanaceous species

Kerchner¹,

Darók²,

Bacskay³

et al. 2015

Acta Biologica Hungarica

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The family Solanaceae includes several melliferous plants, which tend to produce copious amounts of nectar. Floral nectar is a chemically complex aqueous solution, dominated by sugars, but minor components such as amino acids, proteins, flavonoids and alkaloids are present as well. This study aimed at analysing the protein and alkaloid profile of the nectar in seven solanaceous species. Proteins were examined with SDS-PAGE and alkaloids were analyzed with HPLC. The investigation of protein profile revealed significant differences in nectar-protein patterns not only between different plant genera, but also between the three Nicotiana species investigated. SDS-PAGE suggested the presence of several Nectarin proteins with antimicrobial activity in Nicotiana species. The nectar of all tobacco species contained the alkaloid nicotine, N. tabacum having the highest nicotine content. The nectar of Brugmansia suaveolens, Datura stramonium, Hyoscyamus niger and Lycium barbarum contained scopolamine, the highest content of which was measured in B. suaveolens. The alkaloid concentrations in the nectars of most solanaceous species investigated can cause deterrence in honeybees, and the nectar of N. rustica and N. tabacum can be considered toxic for honeybees.

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Section: Alkaloid Content Of the Floral Nectarmentioning

confidence: 57%

Protein and alkaloid patterns of the floral nectar in some solanaceous species

Kerchner¹,

Darók²,

Bacskay³

et al. 2015

Acta Biologica Hungarica

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“…Therefore, they did not allow identification of the spatio‐temporal dynamics of the complete metabolic machinery that is required for nectar production. In order to circumvent the problems that arise from the lack or small size of nectaries in most genetically tractable model species, we used a proteomics approach in combination with biochemical analyses and degenerate primers to study the role of the extrafloral nectaries of the ant‐plant, Acacia cornigera , in the synthesis of the major nectar components: sugars (Heil et al ., ), amino acids (González‐Teuber and Heil, ) and nectarins (Carter and Thornburg, ; González‐Teuber et al ., , ; Nepi et al ., , ; Zha et al ., ). We observed highly dynamic processes in the accumulation and activity of key metabolic enzymes in the nectary tissue, demonstrating that the entire metabolic machinery required for the synthesis of nectar is established and active directly before and during the hours of peak nectar secretion.…”

Section: Introductionmentioning

confidence: 97%

Short‐term proteomic dynamics reveal metabolic factory for active extrafloral nectar secretion by Acacia cornigera ant‐plants

et al. 2012

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These authors have contributed equally to this paper. SUMMARYDespite the ecological and evolutionary importance of nectar, mechanisms controlling its synthesis and secretion remain largely unknown. It is widely believed that nectar is 'secreted phloem sap', but current research reveals a biochemical complexity that is unlikely to stem directly from the phloem. We used the short daily peak in production of extrafloral nectar by Acacia cornigera to investigate metabolic and proteomic dynamics before, during and after 2 h of diurnal secretion. Neither hexoses nor dominating nectar proteins (nectarins) were detected in the phloem before or during nectar secretion, excluding the phloem as the direct source of major nectar components. Enzymes involved in the anabolism of sugars, amino acids, proteins, and nectarins, such as invertase, b-1,3-glucanase and thaumatin-like protein, accumulated in the nectary directly before secretion and diminished quantitatively after the daily secretion process. The corresponding genes were expressed almost exclusively in nectaries. By contrast, protein catabolic enzymes were mainly present and active after the secretion peak, and may function in termination of the secretion process. Thus the metabolic machinery for extrafloral nectar production is synthesized and active during secretion and degraded thereafter. Knowing the key enzymes involved and the spatio-temporal patterns in their expression will allow elucidation of mechanisms by which plants control nectar quality and quantity.

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“…There primers are able to suppress the PCR amplification in the secondary TAIL-PCR since both SP2 and AC1 contain complementary ends and the relatively small ones tend to form a stem-loop structure. It has been successful to isolate genomic flanking sequences of T-DNA insertions from transgenic rice 9 and strawberries 15 , and to determine the full gene structure 26,27 .…”

Section: Hitail-pcr (High-efficiency Tail-pcr)mentioning

confidence: 99%

Randomly primed and ligation-independent PCR methods: High efficiency and cost effective strategies for genome walking and flanking sequence cloning

Z¹,

Liu²,

Bao³

et al. 2013

OA Biotechnology

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Introduction The advent of genomics-based technologies has revolutionized many fields of biological enquiry. However, chromosome walking or flanking sequence cloning is still an important needed tool to isolate the unknown DNA sequences flanked by known sequences or T-DNA insertions in molecular biology research. In this article, we described and compared the principles, efficiency, and applications of the five reported technically less complex methods for chromosome walking or flanking sequence cloning, namely (i) thermal asymmetric interlaced PCR (TAIL-PCR), (ii)high-efficiency TAIL-PCR(hiTAIL-PCR),(iii)fusion primer and nested integrated-PCR (FPNI-PCR),(iv) Sitefinding-PCR and (v)Selfformed Adaptor PCR (SEFA-PCR).While they are all based on the randomly primed PCR without enzyme digestion and ligation procedures, they show difference in efficiency and applications due to the different principles. Methodology The principle, efficiency, and applications of the five enzyme digestion-free methods:(i) TAIL-PCR, (ii) hiTAIL-PCR,(iii) FPNI-PCR, (iv) SiteFinding-PCR and (v) SEFA-PCR are reviewed in detail. Comparative analysis is undertaken to illustrate that the newly developed FPNI-PCR method has shown to be more powerful, effective, time-saving, and accurate than the established conventional reported methods. Conclusion Randomly primed PCR methods represent simplicity, less complex manipulations and possibly will be continuously widely employed by many molecular biology research laboratories. The newly developed FPNI-PCR can be done in a shorter timescale and it is more powerful, effective, and accurate than conventional TAIL-PCR and will be widely used for genome walking and flanking sequence cloning.

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Acidic α-galactosidase is the most abundant nectarin in floral nectar of common tobacco (Nicotiana tabacum)

Cited by 24 publications

References 51 publications

Protein and alkaloid patterns of the floral nectar in some solanaceous species

Protein and alkaloid patterns of the floral nectar in some solanaceous species

Short‐term proteomic dynamics reveal metabolic factory for active extrafloral nectar secretion by Acacia cornigera ant‐plants

Randomly primed and ligation-independent PCR methods: High efficiency and cost effective strategies for genome walking and flanking sequence cloning

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