Acidic polysaccharide isolated from Phellinus linteus induces nitric oxide-mediated tumoricidal activity of macrophages through protein tyrosine kinase and protein kinase C

Kim, Gi‐Young; Oh, Yang-Hyo; Park, Yeong‐Min

doi:10.1016/j.bbrc.2003.08.018

Cited by 76 publications

(32 citation statements)

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“…Hispolon is an active compound isolated from the fungi of Phellinus linteus (10), which is a traditional Chinese medicine and is also known as 'Sanghuang'. Extracts of Phellinus linteus have previously been reported to have immunostimulatory, anti-inflammatory and anticancer activities (10,(20)(21)(22). Numerous studies have demonstrated that hispolon induces apoptosis in human cancer cells (13)(14)(15)(16)22).…”

Section: Discussionmentioning

confidence: 99%

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

2015

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Abstract. Hispolon has been demonstrated to possess analgesic, anti-inflammatory and anticancer activities. However, whether hispolon prevents the invasion of breast carcinoma cells and the underlying mechanisms of its action remain unknown. In the present study, various assays, including a matrigel-based Transwell invasion assay and electrophoretic mobility shift assay, were used to investigate the anti-invasion effect of hispolon and explore its mechanism of action. The results revealed that hispolon inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced migration and invasion of MDA-MB-231 human breast cancer cells at non-toxic concentrations. Hispolon also prevented the TPA-induced secretion of matrix metalloproteinase-9 (MMP-9) and reduced its expression at the transcriptional and translational levels. Furthermore, the phosphorylation of IκBα was reduced by hispolon, which resulted in the suppression of nuclear factor-κB (NF-κB), and p65 phosphorylation and nuclear translocation. An electrophoretic mobility shift assay demonstrated that NF-κB DNA-binding activity was induced by TPA and inhibited by hispolon. In addition, Bay 11-7082, which is a specific inhibitor of NF-κB, functioned in a similar manner as hispolon and blocked the secretion and expression of MMP-9. In conclusion, the results of the present study indicated that hispolon inhibited TPA-induced migration and invasion of MDA-MB-231 cells by reducing the secretion and expression of MMP-9 through the NF-κB signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

2015

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“…These complex polysaccharides have been detected in a variety of different mushroom species and linked to the immunostimulatory and antitumour activities (Wasser, 2002). Therefore, PL stimulated proliferation of T lymphocytes and activated B cells (Kim et al, 1996(Kim et al, , 2003c, and induced maturation of bone marrow-derived dendritic cells and the macrophage response (Kim et al, 2003a). On the other hand, PL demonstrated antiinflammatory effects in lipopolysaccharide-stimulated macrophages (Kim et al, 2006).…”

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confidence: 99%

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling

et al. 2008

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The antitumour activity of a medicinal mushroom Phellinus linteus (PL), through the stimulation of immune system or the induction of apoptosis, has been recently described. However, the molecular mechanisms responsible for the inhibition of invasive behaviour of cancer cells remain to be addressed. In the present study, we demonstrate that PL inhibits proliferation (anchorage-dependent growth) as well as colony formation (anchorage-independent growth) of highly invasive human breast cancer cells. The growth inhibition of MDA-MB-231 cells is mediated by the cell cycle arrest at S phase through the upregulation of p27Kip1 expression. Phellinus linteus also suppressed invasive behaviour of MDA-MB-231 cells by the inhibition of cell adhesion, cell migration and cell invasion through the suppression of secretion of urokinase-plasminogen activator from breast cancer cells. In addition, PL markedly inhibited the early event in angiogenesis, capillary morphogenesis of the human aortic endothelial cells, through the downregulation of secretion of vascular endothelial growth factor from MDA-MB-231 cells. These effects are mediated by the inhibition of serinethreonine kinase AKT signalling, because PL suppressed phosphorylation of AKT at Thr 308 and Ser 473 in breast cancer cells. Taken together, our study suggests potential therapeutic effect of PL against invasive breast cancer.

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“…therefore, plp has been recommended to patients as a natural immunotherapeutic agent without toxicity (23). the immunomodulatory effects of acid polysaccharide isolated from P. linteus (appl) have been correlated with the increased production of nitric oxide (nO) and tumoricidal activity in murine peritoneal macrophages (24). in addition, genistein and staurosporine blocked nO production and tumoricidal activity in response to appl in macrophages, suggesting that appl activates protein tyrosine kinase (ptK) and/or protein kinase c (pKc) signaling in macrophages (24).…”

Section: The Anticancer Activity Of Isolated Compoundsmentioning

confidence: 99%

“…the immunomodulatory effects of acid polysaccharide isolated from P. linteus (appl) have been correlated with the increased production of nitric oxide (nO) and tumoricidal activity in murine peritoneal macrophages (24). in addition, genistein and staurosporine blocked nO production and tumoricidal activity in response to appl in macrophages, suggesting that appl activates protein tyrosine kinase (ptK) and/or protein kinase c (pKc) signaling in macrophages (24). in another study, appl markedly suppressed the metastasis of melanoma cells in mice through the direct inhibition of cell adhesion and invasion (25).…”

Section: The Anticancer Activity Of Isolated Compoundsmentioning

confidence: 99%

Medicinal mushroom Phellinus linteus as an alternative cancer therapy

Slíva

2010

Experimental and Therapeutic Medicine

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Abstract. alternative cancer treatment with nutritional/dietary supplements containing a wide variety of herbal products is on the rise in Western countries. recent epidemiological studies have suggested that mushrooms may prevent against different types of cancers. Phellinus linteus is a well-known Oriental medicinal fungus with a variety of biological activities, including immunomodulatory or direct antitumor activities. the activity of P. linteus and its extracts is associated with the presence of polysaccharides, their peptide/protein complexes and other low molecular weight complexes. polysaccharide fractions isolated from P. linteus were found to be related to the increased activity of immune cells such as the production of cytokines by macrophages and B-cells or the increased cytotoxic activity of natural killer cells. moreover, P. linteus was found to modulate the expression or activity of various genes involved in cell proliferation, apoptosis, angiogenesis, invasive behavior and chemoprevention. Finally, P. linteus extracts demonstrated tumor regression in three independent case reports, suggesting that an extract from P. linteus or a dietary supplement based on the extract from P. linteus may have potential use for the alternative treatment of cancer. Contents1. introduction 2. Phellinus linteus 3. the anticancer activity of isolated compounds 4. the anticancer activity of P. linteus extracts 5. human studies 6. conclusions Introductionthe popularity of complementary and alternative medicine (cam) is steadily increasing among cancer patients, and cam represents one of the fastest growing treatment modalities in the uS (1). the most commonly used cam includes acupuncture, mind-body approaches and dietary supplements. Specifically, among cancer patients, the use of CAM ranges between 30 and 75% worldwide and includes dietary approaches, herbal and other biologically based treatments (2). For example, herbal therapies are used by more than 12% of the uS population each year, resulting in annual out-ofpocket expenses above $5 billion (3). in spite of the popularity of alternative cancer treatments with nutritional/dietary supplements among patients, sometimes based on the anecdotic evidence, cam therapies are in many cases labeled as 'pseudoscience' (4). Therefore, rigorous scientific testing and safety evaluation of dietary supplements must be performed, after which clinicians can recommend the use of a particular dietary supplement (5).Some of the popular, widely used dietary supplements are based on dried mushrooms or mushroom extracts. notably, three recent epidemiological studies from asia demonstrated an inverse correlation between mushroom intake and gastric, gastrointestinal and breast cancer, respectively (6-8). the anticancer activities of mushrooms were usually associated with the stimulation of the immune system by polysaccharides, predominantly β-glucans (9). On the other hand, mushrooms contain minerals, vitamins (e.g., thiamin, riboflavin, ascorbic acid and vitamin d), amino acids and other...

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Acidic polysaccharide isolated from Phellinus linteus induces nitric oxide-mediated tumoricidal activity of macrophages through protein tyrosine kinase and protein kinase C

Cited by 76 publications

References 36 publications

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling

Medicinal mushroom Phellinus linteus as an alternative cancer therapy

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