Acidic Oligosaccharide Sugar Chain, a Marine-Derived Acidic Oligosaccharide, Inhibits the Cytotoxicity and Aggregation of Amyloid Beta Protein

Hu, Jin‐Feng; Geng, Meiyu; Li, Jing; Xin, Xianliang; Wang, Jia; Tang, Michelle; Zhang, Juntian; Zhang, Xiao; Ding, Jian

doi:10.1254/jphs.fpj04004x

Cited by 72 publications

(48 citation statements)

References 29 publications

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“…Although the effect of osmolytes or sugars have been experimentally investigated discretely, 13,31,32 absence of experimental data using a series of osmolytes prompted our study. Lerbret et al 48 reported stabilization of lysozyme in presence of disaccharides using molecular dynamics simulation and stated that ''the addition of sugars significantly reduces lysozyme fluctuations…..''.…”

Section: Discussionmentioning

confidence: 99%

Osmolyte controlled fibrillation kinetics of insulin: New insight into fibrillation using the preferential exclusion principle

Nayak

Lee

McRae

et al. 2009

Biotechnology Progress

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Amyloid proteins are converted from their native-fold to long beta-sheet-rich fibrils in a typical sigmoidal time-dependent protein aggregation curve. This reaction process from monomer or dimer to oligomer to nuclei and then to fibrils is the subject of intense study. The main results of this work are based on the use of a well-studied model amyloid protein, insulin, which has been used in vitro by others. Nine osmolyte molecules, added during the protein aggregation process for the production of amyloid fibrils, slow-down or speed up the process depending on the molecular structure of each osmolyte. Of these, all stabilizing osmolytes (sugars) slow down the aggregation process in the following order: tri > di > monosaccharides, whereas destabilizing osmolytes (urea, guanidium hydrochloride) speed up the aggregation process in a predictable way that fits the trend of all osmolytes. With respect to kinetics, we illustrate, by adapting our earlier reaction model to the insulin system, that the intermediates (trimers, tetramers, pentamers, etc.) are at very low concentrations and that nucleation is orders of magnitude slower than fibril growth. The results are then collated into a cogent explanation using the preferential exclusion and accumulation of osmolytes away from and at the protein surface during nucleation, respectively. Both the heat of solution and the neutral molecular surface area of the osmolytes correlate linearly with two fitting parameters of the kinetic rate model, that is, the lag time and the nucleation rate prior to fibril formation. These kinetic and thermodynamic results support the preferential exclusion model and the existence of oligomers including nuclei and larger structures that could induce toxicity.

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Section: Discussionmentioning

confidence: 99%

Osmolyte controlled fibrillation kinetics of insulin: New insight into fibrillation using the preferential exclusion principle

Nayak

Lee

McRae

et al. 2009

Biotechnology Progress

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“…Additionally, PFF-A inhibited amyloid precursor protein biosynthesis and showed very strong BACE1 inhibitory activity, with a submicromolar IC 50 , making it an interesting potential drug candidate for AD ). An oligosaccharide sugar chain derived from EK inhibited the toxicity induced by the Ab protein in both primary cortical cells and the SH-SY5Y cell line, inhibiting apoptosis and fibril formation and indicating its potency for AD (Hu et al 2004). EM exhibited potent activity against AChE, as did its isolated compounds PG, dibenzo [1,4] dioxine-2,4,7,9-tetraol, and eckol, highlighting its potential as a functional food ingredient for the management of neurodegenerative disorders (Kannan et al 2013).…”

Section: Neuroprotective Activitymentioning

confidence: 97%

Recent advances in pharmacological research on Ecklonia species: a review

2017

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The genus Ecklonia (Lessoniaceae, Phaeophyceae), commonly called kelp (brown algae), is abundant on the coasts of Japan and Korea. During the past few decades, Ecklonia species have received tremendous attention for their wide range of therapeutic properties and multiple health benefits, such as great nutritional value and being rich in vitamins, minerals, dietary fiber, proteins, and polysaccharides. Several novel functional ingredients with diversified biological activities have been isolated and possess antimicrobial, antiviral, hepatoprotective, cardioprotective, anti-inflammatory, neuroprotective, anticarcinogenic, immunomodulatory, hypolipidemic, anti-diabetic, and antioxidant therapeutic properties. The present review discusses the phytochemical, pharmacological, therapeutic, nutritional, and health benefits of different species of genus Ecklonia, as well as their use in the prevention of disease and maintenance of good health.

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“…11 Similarly, an acidic oligosaccharide sugar chain (AOSC) inhibited the formation of β-amyloid induced fibrils and increased cell viability in primary cortical neurons. 12 Sterols [(23E)-3β-hydroxystigmasta-5,23-dien-28-one and (22E)-3β-hydroxycholesta-5,22-dien-24-one], bisindole alkaloids (Racemosa A and Racemosa B) and terpenoids (α-tocospirone) increased the viability of SH-SY5Y cells. 13 …”

Section: A R T I C L E I N F O Abstractmentioning

confidence: 99%

Algae as an Important Resource of Natural Products of Medical and Biotechnological Importance: A Mini-Review

Okoh¹,

Olasehinde²,

Mabinya³

et al. 2017

TJNPR

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Acidic Oligosaccharide Sugar Chain, a Marine-Derived Acidic Oligosaccharide, Inhibits the Cytotoxicity and Aggregation of Amyloid Beta Protein

Cited by 72 publications

References 29 publications

Osmolyte controlled fibrillation kinetics of insulin: New insight into fibrillation using the preferential exclusion principle

Osmolyte controlled fibrillation kinetics of insulin: New insight into fibrillation using the preferential exclusion principle

Recent advances in pharmacological research on Ecklonia species: a review

Algae as an Important Resource of Natural Products of Medical and Biotechnological Importance: A Mini-Review

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