Acidic intracellular Ca2+ stores and caveolae in Ca2+ signaling and diabetes

Guerrero‐Hernández, Agustín; Gallegos-Gomez, Martin Leonardo; Sánchez-Vázquez, Víctor Hugo; López-Méndez, María Cristina

doi:10.1016/j.ceca.2014.08.005

Cited by 9 publications

(4 citation statements)

References 128 publications

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“…It has been demonstrated that cells contain two different types of intracellular Ca 2+ stores, the first is represented primarily by the ER/SR and the other is characterized by having an acidic lumen, and is collectively named “acidic Ca 2+ stores” 31 . A typical characteristic of the acidic Ca 2+ stores is that they can be rapidly depleted by the combination of ionomycin (1 μM) and a substance that can collapse the acidic pH gradient, such as monensin (10 μM) 32 .…”

Section: Resultsmentioning

confidence: 99%

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The Trans Golgi Region is a Labile Intracellular Ca2+ Store Sensitive to Emetine

Gallegos-Gómez

Greotti

López-Méndez

et al. 2018

Sci Rep

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The Golgi apparatus (GA) is a bona fide Ca2+ store; however, there is a lack of GA-specific Ca2+ mobilizing agents. Here, we report that emetine specifically releases Ca2+ from GA in HeLa and HL-1 atrial myocytes. Additionally, it has become evident that the trans-Golgi is a labile Ca2+ store that requires a continuous source of Ca2+ from either the external milieu or from the ER, to enable it to produce a detectable transient increase in cytosolic Ca2+. Our data indicates that the emetine-sensitive Ca2+ mobilizing mechanism is different from the two classical Ca2+ release mechanisms, i.e. IP3 and ryanodine receptors. This newly discovered ability of emetine to release Ca2+ from the GA may explain why chronic consumption of ipecac syrup has muscle side effects.

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Section: Resultsmentioning

confidence: 99%

“…We decided then to study whether acidic Ca 2+ stores were targeted by emetine, with the knowledge that Ca 2+ handling in the acidic compartment is dependent on the proton gradient generated across this membrane 31 . We studied the role of the acidic Ca 2+ stores with the combination of ionomycin and monensin.…”

Section: Discussionmentioning

confidence: 99%

The Trans Golgi Region is a Labile Intracellular Ca2+ Store Sensitive to Emetine

Gallegos-Gómez

Greotti

López-Méndez

et al. 2018

Sci Rep

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“…There are some indications that insufficient calcium and vitamin D intake may alter the balance between intracellular and extracellular calcium pools in β-cells, leading to reduction in insulin secretion [19]. Calcium as a second messenger has an important signalling role for insulin action in insulin-responsive tissues [20], and some studies have reported an inverse association between insulin resistance and intracellular cytoplasmic calcium levels in insulin target tissues [19]. Insulin, in turn, may suppress calcium tubular reabsorption [21] and thus reduce calcium levels.…”

Section: Discussionmentioning

confidence: 99%

Trace element status in patients with type 2 diabetes in Norway: The HUNT3 Survey

Simic

Hansen

Åsvold

et al. 2017

Journal of Trace Elements in Medicine and Biology

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Several epidemiological studies have indicated that a number of trace elements may play a role in type 2 diabetes (T2D). We investigated the association between prevalent T2D and the concentrations of 25 trace elements in whole blood, and the relationships between T2D duration and blood levels of the trace elements that we found to be related to T2D prevalence. In this population based case-control study, 267 patients with self-reported T2D and 609 controls (frequency matched), were selected from the third Nord-Trøndelag Health Survey. Trace element blood levels were determined by high resolution inductively coupled plasma-mass spectrometry. Multivariable conditional logistic regression and multivariable linear regression were used to estimate associations. The prevalence of T2D was positively associated with boron, calcium and silver, and inversely associated with indium, lead and magnesium (P<0.05). We found no statistical evidence for associations between blood levels of arsenic, bromine, cadmium, cesium, chromium, copper, gallium, gold, manganese, mercury, molybdenum, nickel, rubidium, selenium, strontium, tantalum, thallium, tin and zinc and T2D prevalence. After corrections for multiple testing, associations remained significant for calcium and lead (Q<0.05), and borderline significant for magnesium, silver and boron. With increasing disease duration, higher calcium levels were observed (P<0.05). This study suggests an association between prevalent T2D and blood levels of boron, calcium, indium, lead, magnesium and silver.

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“…However, it appears that there are two different mechanisms of Ca 2+ release, one associated with the ER (IP 3 Rs) and the other with acidic Ca 2+ stores [32]. The latter mechanism has been reviewed elsewhere [181]. Agonists like bradykinin, histamine, thrombin, Angiotensin II and ATP via G protein coupled receptors lead to IP 3 production from phospholipid PIP 2 by activation of a phospholipase C, the generated IP 3 in turn activates IP 3 Rs located in the ER and result in Ca 2+ release and a transient elevation of [Ca 2+ ] i .…”

Section: Er Ca 2+ Release Channelsmentioning

confidence: 99%