Acid stress management by Cronobacter sakazakii

Álvarez‐Ordóñez, Avelino; Cummins, Conor; Deasy, Thérèse; Clifford, Tanya; Begley, Máire; Hill, Colin

doi:10.1016/j.ijfoodmicro.2014.03.001

Cited by 46 publications

(18 citation statements)

References 47 publications

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“…6b), and the consensus sequence of CpxR site was also found upstream of the fabA and fabB genes in additional bacteria species (Table 1). Additionally, Cronobacter sakazakii clone carrying transposon insertion in cpxR gene was identified as acid-sensitive mutant 49 . All these facts suggest this ATR system functioning in exponential phase is highly conserved across bacteria species.…”

Section: Resultsmentioning

confidence: 99%

An acid-tolerance response system protecting exponentially growing Escherichia coli

et al. 2020

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The ability to grow at moderate acidic conditions (pH 4.0-5.0) is important to Escherichia coli colonization of the host's intestine. Several regulatory systems are known to control acid resistance in E. coli, enabling the bacteria to survive under acidic conditions without growth. Here, we characterize an acid-tolerance response (ATR) system and its regulatory circuit, required for E. coli exponential growth at pH 4.2. A two-component system CpxRA directly senses acidification through protonation of CpxA periplasmic histidine residues, and upregulates the fabA and fabB genes, leading to increased production of unsaturated fatty acids. Changes in lipid composition decrease membrane fluidity, F 0 F 1 -ATPase activity, and improve intracellular pH homeostasis. The ATR system is important for E. coli survival in the mouse intestine and for production of higher level of 3-hydroxypropionate during fermentation. Furthermore, this ATR system appears to be conserved in other Gram-negative bacteria.

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Section: Resultsmentioning

confidence: 99%

An acid-tolerance response system protecting exponentially growing Escherichia coli

et al. 2020

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“…In this study, 953 clones were obtained by transposition-mediated random mutagenesis, and a single non-motile clone was identified, whose insertion site was mapped within the fliF gene. Several studies, have shown that random mutagenesis by transposition (EZ-Tn5 TM <Kan-2>Tnp Transposome TM ) is an efficient system to generate mutants, allowing the easy identification of their phenotype and mapping [24][25][26][27]. This mutation system has shown a high efficiency ratio in other bacteria, such as E. coli 10 5 > Salmonella enterica serovar Typhimurium 10 4 > Proteus vulgaris 10 3 > Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Proteomics Profile of Cronobacter sakazakii and fliF Mutant: Adherence and Invasion to Mouse Neuroblastoma Cells

Esteban-‬Kenel

Curiel-Quesada²,

Quezada

et al. 2019

Preprint

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Background: Cronobacter sakazakii is an opportunistic foodborne pathogen associated with necrotizing enterocolitis, bacteraemia, and meningitis in infants. A comparative proteomic study of C . sakazakii ATCC BAA-894 (CS WT) and isogenic mutant of flagella were performed; including the ability of both strains to adhere and invade to N1E-115 cells. Results: To achieve this goal, a non-motile C. sakazakii ATCC BAA-894 fliF ::Tn5 (CS fliF ::Tn5) strain was generated using an EZ-Tn5Tnp Transposome kit. Analysis of differential protein expression showed that 81.49% (361/443) of the proteins were identified in both strains, 8.35% (37/443) were exclusively expressed in the CS WT strain and 10.16% (45/443) in the CS fliF ::Tn5 strain. The main exclusive proteins from the CS WT strain were classified into the following subcategories: “cell motility” and “signal transduction mechanisms”. In contrast, the exclusive proteins from the CS fliF ::Tn5 strain were classified into the following subcategories: “intracellular trafficking, secretion, and vesicular transport”, “replication, recombination, and repair”, “nucleotide transport and metabolism”, “carbohydrate transport and metabolism”, “coenzyme transport and metabolism”, and “lipid transport and metabolism”. Expression of the Cpa protein was shared by both strains but was more abundant in the CS WT strain than in the CS fliF ::Tn5 strain. Significant increases (p=0.0001) in adherence to N1E-115 cells were observed for the non-motile CS fliF ::Tn5 strain, with 31.3×10 6 CFU/mL, relative to the CS WT strain, with 14.5×10 6 CFU/mL. Additionally, for infection of N1E-115 cells, the CS WT strain showed a 0.17% invasion frequency, which was significantly increased (p=0.01) compared to that of the non-motile CS fliF ::Tn5 strain. Conclusion : The proteins involved in motility were mainly identified by proteomic analysis in CS WT strain when compare to CS fliF ::Tn5 strain. Our data showed that flagella are required to promote invasion to N1E-115 cells and absence of flagella significantly increases the adherence to N1E-115 cells when compare to CS WT strain.

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“…Also, in Cronobacter , a cross-protection is observed for several stresses. For example, Alvarez-Ordóñez et al (2014) showed that C. sakazakii exposed to acid stress produces activation of several genes encoding chaperone proteins, as DnaJ also involved in heat stress response. Consequently, the way that bacteria undergo environmental perturbation as performed in this study could also promote their survival in gastrointestinal tract until epithelium cells.…”

Section: Discussionmentioning

confidence: 99%

Caco-2 Invasion by Cronobacter sakazakii and Salmonella enterica Exposed to Drying and Heat Treatments in Dried State in Milk Powder

et al. 2017

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Due to the ability of foodborne pathogens to survive in low moisture food, the decontamination of milk powder is an important issue in food protection. The safety of food products is, however, not always insured and the different steps in the processing of food involve physiological and metabolic changes in bacteria. Among these changes, virulence properties may also be affected. In this study, the effect of drying and successive thermal treatments on the invasion capacity of Salmonella Typhimurium, Salmonella Senftenberg, and Cronobacter sakazakii was assessed. Bacteria were dried on milk powder at three different water activity levels (0.25, 0.58, and 0.80) and heated at two different temperatures (90°C and 100°C) for 30 and 120 s. After recovery, stressed bacterial populations were placed in contact with Caco-2 cells to estimate their invasion capacity. Our results show that drying increases the invasion capacity of foodborne pathogens, but that heat treatment in the dried state did not exert a selective pressure on bacterial cells depending on their invasion capacity after drying. Taken together, our findings add to the sum of knowledge on food safety in dried food products and provide insight into the effects of food processing.

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Acid stress management by Cronobacter sakazakii

Cited by 46 publications

References 47 publications

An acid-tolerance response system protecting exponentially growing Escherichia coli

An acid-tolerance response system protecting exponentially growing Escherichia coli

Proteomics Profile of Cronobacter sakazakii and fliF Mutant: Adherence and Invasion to Mouse Neuroblastoma Cells

Caco-2 Invasion by Cronobacter sakazakii and Salmonella enterica Exposed to Drying and Heat Treatments in Dried State in Milk Powder

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