Acid-Soluble Nucleotides in the Unfertilized Eggs of the Sea-Urchin Paracentrotus lividus.

Nilsson, Rune; Marton, Josef; Smith-Kielland, I.; Sömme, R.; Stenhagen, Einar; Palmstierna, H.

doi:10.3891/acta.chem.scand.13-0395

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1963

1975

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The development of a process for recovering nucleotides from Bakers' yeast

Munden

Crook

Donald

1963

Biotech & Bioengineering

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A process has been developed giving over-all yields of about 50yo: which could be increased to about 80% with higher material costs and processing time. The preferred method of recovering nucleotides from the cytoplasm has been thermal extraction by means of a heat exchanger followed by filtration, protein precipitation, and carbon adsorption. The concentrated eluate from the carbon is absorbed onto Ihwex 1 ion-exchauge resin, and groups oi nucleotides are removed by pH-defined eluents and recovered by il second carbon adsorption. Finally, standard ion-exchange resolutions have been used to recover individual nucleotides. Other biochemicals in the extract have been investigated, and preliminary work has been done on the combination of nucleotide extraction with the recovery of nucleic acids from the residual rells.

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