Acid Protease in Nepenthes

Nakayama, Shigeru; Amagase, Shizuko

doi:10.2183/pjab1945.44.358

Cited by 33 publications

(6 citation statements)

References 2 publications

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“…Moreover, tests with silver nitrate showed Cl-to be present in the secretions of many pitchers. Recently, Nakayma and Amagase [2] described the partial purification of the acid protease from opened pitchers and in contrast with the results of earlier authors [3,4] and ours [ 1 ] , the Japanese workers could not find any proteolytic activity in the secretions of unopened pitchers. Thus, they could not exclude the bacterial origin of their protease.…”

Section: Resultscontrasting

confidence: 91%

Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin

Jentsch

1972

FEBS Letters

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Section: Resultscontrasting

confidence: 91%

Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin

Jentsch

1972

FEBS Letters

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“…AP have been purified and characterised from sterile pitcher fluid of several Nepenthes species ( Jentsch, 1972 ; Tokes, Woon & Chambers, 1974 ). In a study conducted by Nakayama & Amagase (1968) , a protease from pooled pitcher fluids of N. mixta and N. maxima was only partially purified and characterised due to insufficient amount. Amagase (1972) investigated aspartic proteases found in N. ampullaria, N. mixta, N. rafflesiana, N. maxima , and N. dyeriana compared to leaf extract from Drosera peltata .…”

Section: Secreted Proteases In Different Families Of Carnivorous Planmentioning

confidence: 99%

Discovery of digestive enzymes in carnivorous plants with focus on proteases

Ravee

Salleh

Goh

2018

PeerJ

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BackgroundCarnivorous plants have been fascinating researchers with their unique characters and bioinspired applications. These include medicinal trait of some carnivorous plants with potentials for pharmaceutical industry.MethodsThis review will cover recent progress based on current studies on digestive enzymes secreted by different genera of carnivorous plants: Drosera (sundews), Dionaea (Venus flytrap), Nepenthes (tropical pitcher plants), Sarracenia (North American pitcher plants), Cephalotus (Australian pitcher plants), Genlisea (corkscrew plants), and Utricularia (bladderworts).ResultsSince the discovery of secreted protease nepenthesin in Nepenthes pitcher, digestive enzymes from carnivorous plants have been the focus of many studies. Recent genomics approaches have accelerated digestive enzyme discovery. Furthermore, the advancement in recombinant technology and protein purification helped in the identification and characterisation of enzymes in carnivorous plants.DiscussionThese different aspects will be described and discussed in this review with focus on the role of secreted plant proteases and their potential industrial applications.

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“…They have primarily been reported from the pitcher fluid of Nepenthes plants [24,25], and account for the majority of protease activity in the fluid [26]. Purification and characterization of proteases from the pitcher fluid has confirmed that the protease activity was indeed mainly ascribed to nepenthesins [27][28][29]. Subsequently, cDNA clones of nepenthesins were characterized from Nepenthes alata [30] and N. gracilis [31,32].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Nepenthesin HvNEP-1 in Barley Endosperm Reduces Fusarium Head Blight and Mycotoxin Accumulation

et al. 2020

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Fusarium head blight (FHB) causes substantial losses of yield and quality in grains, both in the field and in post-harvest storage. To date, adequate natural genetic resistance is not available for the control of FHB. This study reports the cloning and overexpression of a barley (Hordeum vulgare L.) antifungal gene, nepenthesin 1 (HvNEP-1), in the endosperm of barley grains. Transgenic barley lines overexpressing HvNEP-1 substantially reduced FHB severity and disease progression after inoculation with Fusarium graminearum or Fusarium culmorum. The transgenic barley also showed reduced accumulation of the mycotoxin deoxynivalenol (DON) in grain, far below the minimum value allowable for food. Semi-field evaluation of four HvNEP-1 transgenic lines revealed substantial reduction of FHB severity and progression as compared with the control H. vulgare cultivar Golden promise (GP) plants. Our study demonstrated the utility of HvNEP-1 for the control of FHB in barley, and possibly other grains such as wheat and maize.

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Acid Protease in Nepenthes

Cited by 33 publications

References 2 publications

Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin

Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin

Discovery of digestive enzymes in carnivorous plants with focus on proteases

Overexpression of Nepenthesin HvNEP-1 in Barley Endosperm Reduces Fusarium Head Blight and Mycotoxin Accumulation

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