Acid Profile in Carious Dentin

Hojo, Sachiko; Takahashi, Nobuhiro; Yamada, Tadashi

doi:10.1177/00220345910700030501

Cited by 50 publications

(37 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Assuming, therefore, that the chromophore is organic, is it a component of the original dentine matrix or is it an exogenous molecule introduced during the carious attack? The 1989] major bacterial products associated with dentinal decay are lactic acid [Hojo et al, 1991] and proteolytic enzymes. pH is reduced in the lesion, but pH changes per se do not affect the AF signal [van der Veen and ten Bosch, 1996].…”

Section: Discussionmentioning

confidence: 99%

Autofluorescence and Mineral Content of Carious Dentine: Scanning Optical and Backscattered Electron Microscopic Studies

Banerjee

Boyde²

1998

Caries Res

View full text Add to dashboard Cite

The aim of this study was to investigate the extent of natural intrinsic fluorescence in carious human dentine and any correspondence of such autofluorescence (AF) to the mineral distribution within the lesion. Two investigative techniques were used, both employing the same sample set and fields in diamond-polished block surfaces of polymethylmethacrylate-embedded carious teeth. AF at emission wavelengths > 515 nm, excited by 488-nm laser light, was assessed using a confocal laser scanning optical microscope (CLSM), the recordings made under standard operating conditions. The relative mineral content was assessed using digital backscattered scanning electron microscopy (20 kV BSE-SEM). The AF intensity correlated with the level of demineralisation as seen by BSE, but the depth of the lesion as seen by CLSM was significantly greater implying that the AF is not directly related to the mineral component.

show abstract

Section: Discussionmentioning

confidence: 99%

Autofluorescence and Mineral Content of Carious Dentine: Scanning Optical and Backscattered Electron Microscopic Studies

Banerjee

Boyde²

1998

Caries Res

View full text Add to dashboard Cite

show abstract

“…The microbiotas in dentin caries consist of both acidogenic/aciduric bacteria, such as Streptococcus mutans , Lactobacillus , and Bifidobacterium species, and proteolytic/amino acid-degrading bacteria, such as Prevotella and Propionibacterium species . Reflecting this microbiological diversity, dentin caries lesions were reported to contain a variety of organic acids, and their pH was found to vary markedly [Hojo et al, 1991[Hojo et al, , 1994.…”

Section: Microbial Ecosystem Of Dentin and Root Cariesmentioning

confidence: 99%

“…In particular, Prevotella intermedia is known to exhibit protease activity at a wide range of pH (from neutral to acidic) as well as acid-producing activity [Takahashi and Schachtele, 1990]. No studies have identified a bacterial involvement in the initial degradation of dentin matrix, but the detection of bacterial metabolites in carious dentin cavities [Hojo et al, 1991[Hojo et al, , 1994 suggests that bacteria digest partly degraded dentin matrix and metabolize it into organic acids. Nevertheless, bacterial acidification is essential for the acid-induced demineralization of tooth minerals, the acid-induced denaturation of exposed and partly degraded proteins, and the acid-induced activation of host-derived proteases.…”

Section: Protein Degradation By Host-and Bacteria-derived Proteasesmentioning

confidence: 99%

Ecological Hypothesis of Dentin and Root Caries

2016

View full text Add to dashboard Cite

Recent advances regarding the caries process indicate that ecological phenomena induced by bacterial acid production tilt the de- and remineralization balance of the dental hard tissues towards demineralization through bacterial acid-induced adaptation and selection within the microbiota - from the dynamic stability stage to the aciduric stage via the acidogenic stage [Takahashi and Nyvad, 2008]. Dentin and root caries can also be partly explained by this hypothesis; however, the fact that these tissues contain a considerable amount of organic material suggests that protein degradation is involved in caries formation. In this review, we compiled relevant histological, biochemical, and microbiological information about dentin/root caries and refined the hypothesis by adding degradation of the organic matrix (the proteolytic stage) to the abovementioned stages. Bacterial acidification not only induces demineralization and exposure of the organic matrix in dentin/root surfaces but also activation of dentin-embedded and salivary matrix metalloproteinases and cathepsins. These phenomena initiate degradation of the demineralized organic matrix in dentin/root surfaces. While a bacterial involvement has never been confirmed in the initial degradation of organic material, the detection of proteolytic/amino acid-degrading bacteria and bacterial metabolites in dentin and root caries suggests a bacterial digestion and metabolism of partly degraded matrix. Moreover, bacterial metabolites might induce pulpitis as an inflammatory/immunomodulatory factor. Root and dentin surfaces are always at risk of becoming demineralized in the oral cavity, and exposed organic materials can be degraded by host-derived proteases contained in saliva and dentin itself. New approaches to the prevention and treatment of root/dentin caries are required.

show abstract

“…This they achieve by acidic dissolution of hydroxyapatite, 18,19 through cracks in the tooth crown, 20 or through exposed dentinal tubules in traumatized teeth with a disconnected nerve-vessel bundle. 21 In addition, microorganisms can reach the pulp space via the apical foramen in cases of severe periodontal disease.…”

Section: Infection Of the Root Canal Spacementioning

confidence: 99%