Acid phosphatase reaction and Alcian Blue-Safranin staining of rat peritoneal mast cells.

Cytochemical Study of Phosphatases in Globule Leucocytes Isolated from Rat Tracheal Epithelium

“…In the peritoneal control cells, acid-phosphatase-positive granules in macrophages, neutrophils and lympho cytes confirm previous observations [19], The relatively few positive granules in peritoneal mast cells are in agree ment with the electron-microscopic results of Miyashita et al [24], indicating that only the Alcian-blue-positive gran ules of mast cells contain acid phosphatase.…”

Relationship between the staining property of mast cell granule with alcian blue-safranin O and toluidine blue O, and the content of mast cell protease I in the granule of rat peritoneal mast cell.

“…Recently, Miyashita et al showed that the alcian blue positive granules correspond to the reticular granules with positive acid phosphatase reaction and the safranin positive granules correspond to the compact granules with negative acid phosphatase reaction (7). With these results the secretion of granular content suggested by Takeoka (12,13) can be related to the secretion of RMCP I.…”

“…The author tried to find techniques which could enable us to examine more precisely and reasonably the content of RMCP I of mast cell granules in the various conditions. Alcian blue-safranin double staining method is a well established method (2,3,6,7) and the author examined the relationship between the stainability of the granules of connective tissue mast cells and the RMCP I content of granules expressed by the electron immunohistochemical method.…”