Acid phosphatase activity in the isolated brush border membrane of the tapeworm, <i>Hymenolepis diminuta</i>: Partial, characterization and differentiation from the alkaline phosphatase activity

Pappas, Peter W.

doi:10.1002/jcb.240370407

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…Changes in the tegumental architecture on treatment with gold nanoparticles suggest that the phytopathogenic fungal products bring about permeability changes in the tegument of the cestodes. In a number of helminth parasites AcPase, AlkPase, ATPase and 5′-Nu are known to be closely associated with the tegument, subtegument, somatic musculature and gut [58]–[61]. As revealed in the present in vitro study, alterations occurred in the enzyme activity of the parasite after treatment with gold nanoparticles, in synchrony with the effectiveness of the reference botanicals and the reference drug tested in previous studies.…”

Section: Discussionsupporting

confidence: 80%

Anthelmintic Efficacy of Gold Nanoparticles Derived from a Phytopathogenic Fungus, Nigrospora oryzae

Kar¹,

Murmu²,

Saha

et al. 2014

PLoS ONE

100

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Exploring a green chemistry approach, this study brings to the fore, the anthelmintic efficacy of gold nanoparticles, highlighting the plausible usage of myconanotechnology. Gold nanoparticles of ∼6 to ∼18 nm diameter were synthesized by treating the mycelia-free culture filtrate of the phytopathogenic fungus with gold chloride. Their size and morphology were confirmed by UV-Vis spectroscopy, DLS data, AFM and TEM images. The XRD studies reveal a crystalline nature of the nanoparticles, which are in cubic phase. The FTIR spectroscopic studies before and after the formation of nanoparticles show the presence of possible functional groups responsible for the bio-reduction and capping of the synthesized gold nanoparticles. The latter were tested as vermifugal agents against a model cestode Raillietina sp., an intestinal parasite of domestic fowl. Further, ultrastructural and biochemical parameters were used to corroborate the efficacy study.

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Section: Discussionsupporting

confidence: 80%

Anthelmintic Efficacy of Gold Nanoparticles Derived from a Phytopathogenic Fungus, Nigrospora oryzae

Kar¹,

Murmu²,

Saha

et al. 2014

PLoS ONE

100

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“…In a number of helminth parasites, AcPase and AlkPase have been detected histochemically and found to be closely associated with the tegument, subtegument, somatic musculature, gut and cuticle (Pappas 1988;Fetterer and Rhoads 2000). AcPase is usually associated with lysosomes and AlkPase is regarded as indicative of membrane transport mechanisms (Barrett 1981).…”

Section: Discussionmentioning

confidence: 99%

Antiparasitic activity of methanolic extract of Acacia oxyphylla (Leguminosae) against Raillietina echinobothrida

Dasgupta

Roy

2010

J Parasit Dis

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Acacia oxyphylla has been used traditionally by the natives of Mizoram against intestinal worm infections. In the present study, the crude methanolic extract of the plant was tested in vitro on the cestode parasite Raillietina echinobothrida to evaluate its potential anthelmintic efficacy. The test parasites were exposed to varying concentrations of the plant extract in phosphate buffered saline and they all slipped into a flaccid paralytic state at varying periods of time post incubation. Ultrastructural observations on the paralysed worms revealed wide scale destruction of the parasite tegument with intense vacuolization of the syncytium and swellings of the basal lamina accompanied by deformities in the cell organelles. To determine the exact mode of action of the botanicals on the parasite body surface, the tegumental enzymes viz. acid phosphatase (AcPase), alkaline phosphatase (AlkPase) and adenosine triphosphatase (ATPase) were investigated. A prominent decrease in the phosphatase activity, in comparison to the untreated control parasites was noticeable. In view of the alterations in the structural and functional integrity of the tegument, occurring in the treated parasites, it may be assumed that the changes transpire because of a compromise in the permeability of the tegument under the influence of the test plant-derived active chemical principles.

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“…APA is often distributed among several distinct isozymes which are, themselves, distributed among the cytoplasm, organelles, and plasma membrane of the cell (SENSABAUGH, 1975). It has been reported recently that the tegument of the tapeworm, Hymenolepis diminuta, contains a membranebound APA (PAPPAS, 1988), and this paper reports on the partial purification and characterization of a second APA in this helminth species.…”

Section: Introductionmentioning

confidence: 90%

“…Assays were terminated by adding 1 ml of 1 N NaOH and heating the reaction mixture at 95°C for 5 min. The amount of p-nitrophenol (PNP) liberated was quantified at 405 nm using a millimolar extinction coefficient of 17-5 (PAPPAS, 1982(PAPPAS, , 1988. Results of these assays were expressed in "units" of APA, where a "unit" was defined as the amount of enzyme liberating 1 nmol min" 1 of PNP.…”

Section: Methodsmentioning

confidence: 99%

“…An aliquot of the purified preparation was diluted to 480 \i\ with 50 mM acetate buffer, pH 5-0, and the reaction was initiated by the addition of 20 ul of the appropriate substrate. Following incubation at 37°C for an appropriate time, reactions were terminated by the addition of 0-8 ml of a Malachite Green-ammonium molybdate solution and the amount of inorganic phosphate (P ( ) liberated was quantified (LANZETTA et al, 1979;PAPPAS, 1988). Specific activities were calculated in units of umol Pj liberated mg~' protein min '.…”

Section: Methodsmentioning

confidence: 99%

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Partial purification and characterization of a soluble acid phosphatase from the tapeworm,Hymenolepis diminuta

Bumbulis

Pappas

1991

J. Helminthol.

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An acid phosphatase activity (APA; EC 3.1.3.2) was demonstrated in homogenates of adult Hymenolepis diminuta. The APA was soluble based on the observation that it did not sediment at 130 000 g. APA was partially purified using a combination of differential centrifugation, ammonium sulphate precipitation, chloroform extraction, and gel and fast-protein-liquid-chromatography. This combination of techniques resulted in a preparation with a specific activity approximately 500 times greater than the crude enzyme preparation. The temperature and pH optima of the partially purified APA were 44°C and pH 5·0. The enzyme appeared to be a monomer with a molecular weight of approximately 62 000. APA had a higher affinity for a greater activity towards aromatic than aliphatic phosphoesters and phosphoryl transferase activity was demonstrable using 1-butanol and ethylene glycol as acceptors. APA was inhibited significantly by sodium dodecyl sulphate, fluoride, molybdate and tartrate, but CuSO4 and Fast Garnet GBC were poor inhibitors. The precise cellular localization and function of this enzyme remains unknown since it possesses characteristics of both cytoplasmic and lysosomal APA's of other organisms.

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Acid phosphatase activity in the isolated brush border membrane of the tapeworm, Hymenolepis diminuta: Partial, characterization and differentiation from the alkaline phosphatase activity

Cited by 12 publications

References 17 publications

Anthelmintic Efficacy of Gold Nanoparticles Derived from a Phytopathogenic Fungus, Nigrospora oryzae

Anthelmintic Efficacy of Gold Nanoparticles Derived from a Phytopathogenic Fungus, Nigrospora oryzae

Antiparasitic activity of methanolic extract of Acacia oxyphylla (Leguminosae) against Raillietina echinobothrida

Partial purification and characterization of a soluble acid phosphatase from the tapeworm,Hymenolepis diminuta

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