2001
DOI: 10.1128/iai.69.3.1679-1686.2001
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Acid-Induced Gene Expression in Helicobacter pylori : Study in Genomic Scale by Microarray

Abstract: To understand the RNA expression in response to acid stress of Helicobacter pylori in genomic scale, a microarray membrane containing 1,534 open reading frames (ORFs) from strain 26695 was used. Total RNAs of H. pylori under growth conditions of pH 7.2 and 5.5 were extracted, reverse transcribed into cDNA, and labeled with biotin. Each microarray membrane was hybridized with cDNA probe from the same strain under two different pH conditions and developed by a catalyzed reporter deposition method. Gene expressio… Show more

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Cited by 117 publications
(123 citation statements)
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“…Total RNA (40 mg) labeling, microarray hybridization, colorimetric detection, and densitometry were performed and analyzed as described previously. 11,46 Expression level of 23S rRNA on each membrane was used as an internal standard for normalization. Processed microarray data files have been deposited in the National Center for Biotechnology Information under GEO accession number GSE69327.…”
Section: Methodsmentioning
confidence: 99%
“…Total RNA (40 mg) labeling, microarray hybridization, colorimetric detection, and densitometry were performed and analyzed as described previously. 11,46 Expression level of 23S rRNA on each membrane was used as an internal standard for normalization. Processed microarray data files have been deposited in the National Center for Biotechnology Information under GEO accession number GSE69327.…”
Section: Methodsmentioning
confidence: 99%
“…A number of studies have sought to examine the transcriptional profile of bacteria grown under acidic and/or basic conditions (2,16,18,37,53,84). The small degree of overlap between these studies affirms the importance of using multiple approaches to characterize these responses as thoroughly as possible.…”
mentioning
confidence: 99%
“…These studies revealed that low pH changes the expression of genes involved in energy generation and metabolism, reflecting a switch to metabolic processes that minimize acid production (18,37,53). In addition, pH affects the expression of genes involved in motility and chemotaxis (2,16,37,53,84) as well as genes that encode products that take up and/or export H ϩ (e.g., ATPase and electron transport chain components) (2,16,18,37,53). These studies also have shown that bacterial acid stress responses show some overlap with oxidative stress (16,53,67) and heat shock (2,18,37,67) responses.…”
mentioning
confidence: 99%
“…8 and 9). Whole genome genotyping of Hp1 indicated that it contains the 93 of 95 H. pylori genes reported to be regulated by acid, suggesting that it is equipped to respond to the presence or absence of acid in its microenvironments (9)(10)(11). Our results demonstrate that this bacterium seeks out niches with sparse parietal cell populations and underscore the point that understanding the forces that shape the destiny of an infection requires a commitment to defining host and microbial responses as a function of the various gastric milieus that coexist within a single host.…”
mentioning
confidence: 99%