Acid Hydrolysis of Pectin for Cell Growth of Cupriavidus necator

Locatelli, Gabriel Olivo; Silva, Gabriella Dias da; Finkler, Leandro; Finkler, Christine Lamenha Lu

doi:10.3923/biotech.2012.29.36

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…Similar methods of preparation are found in the literature. 19 However, they involve a greater number of reagents (i.e., oxidizing or reducing agents, binders, organic solvents, polymerizing agents) and require special handling conditions (i.e., controlled atmosphere and humidity) or a greater number of steps (i.e., mixing, heating, volume reduction, grinding, sieving). [23][24][25] This pectin-induced method is a much simpler and inexpensive alternative, which adds value to the organic residues from the fruit pulp industry.…”

Section: Resultsmentioning

confidence: 99%

“…The magnetic iron oxides (MIOs) were prepared by using the following commercially available reagents in analytical grade, purchased from Vetec Fine Chemicals and Sigma-Aldrich Company: iron ( 19 consisting of poly(galacturonic acid) connected by 1,4-β-glucoside bonds. The minimum formula is [C 5 H 7 O 4 (COOH) m-1 (COOCH 3 ) m ] n , where m refers to the degree of esterification as deduced from the formula (14.02m + 176.13) and n is the average number of repeating units.…”

Section: Materials and Reagentsmentioning

confidence: 99%

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Facile Synthesis Strategy to Create Mesoporous Magnetic Iron Oxides Using Pectin‑Based Precursors

Mariani¹,

Dalpasquale²,

Toma³

et al. 2016

J. Braz. Chem. Soc.

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Magnetic iron oxides are of great scientific and technological relevance. Therefore, herein, we report the research on developing methodologies for their sustainable and reproducible preparation. We discuss the synthesis of iron oxides involving citrus pectin as a source of energy (42 MJ kg-1) to promote the formation of the magnetic phases. The method involved two simple steps: gelation of the pectin (80 °C, 4 h) with iron nitrate nonahydrate [Fe(NO 3) 3 .9H 2 O], and calcination (600 °C, 1 h). X-ray diffraction indicated the major formation of hematite (α-Fe 2 O 3) and ferrite phases. Microscopy images show materials with an average size below 50 nm. Textural properties indicated pore sizes of 7-30 nm while magnetic measurements indicated the formation of soft and hard oxides (coercivity between 90 and 400 Oe) and high magnetization saturation (50 emu g-1).

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Section: Resultsmentioning

confidence: 99%

Section: Materials and Reagentsmentioning

confidence: 99%

Facile Synthesis Strategy to Create Mesoporous Magnetic Iron Oxides Using Pectin‑Based Precursors

Mariani¹,

Dalpasquale²,

Toma³

et al. 2016

J. Braz. Chem. Soc.

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“…Importantly, structure, recalcitrance, and composition are the main factors that will determine the degree of pretreatment of feedstocks necessary for the formulation of a suitable fermentation media. ,− For example, an extraction is often required if the targeted carbon source is embedded in a complex matrix. If the carbon source is hydrophobic as in the case of lipids, solubilization via the addition of surfactants might be required to improve carbon availability to the cells. ,, Due to the presence of polysaccharides in alternative feedstocks, hydrolysis is also often an essential pretreatment step, as many PHA-producer organisms may not be able to readily process complex polysaccharides such as cellulose. − Finally, the detoxification or dilution of certain waste streams may be also required to avoid inhibition of cell growth or product synthesis. In some cases, and even though the pretreatment of these alternative feedstocks can be regarded as an additional cost, these strategies can significantly decrease the overall cost of the production process when compared to the costs of utilizing pure carbon sources.…”

Section: Challenges For the Commercialization Of Phasmentioning

confidence: 99%

Polyhydroxyalkanoates Production: A Challenge for the Plastic Industry

Guzmán-Lagunes,

Wongsirichot,

Winterburn

et al. 2023

Ind. Eng. Chem. Res.

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The accumulation of petroleum-based plastics in the environment has heightened the necessity of finding alternative materials with similar properties and lower environmental impact. Polyhydroxyalkanoates (PHAs) are a group of naturally occurring polyesters that have been investigated for several decades as potential substitutes for commonly used plastics. PHAs are biodegradable and biocompatible polymers derived from renewable resources. From this point of view, their industrial implementation represents an attractive strategy to reduce plastic pollution and petroleum dependency. Nevertheless, the high production costs related to these materials have considerably hindered their broader use and commercialization. In this regard, several approaches have been proposed to turn their production competitive from a commercial perspective. For instance, several studies have scrutinized the different production steps of PHAs in terms of economic viability. Reported strategies include optimization of fermentation processes; selection, isolation, and genetic modification of microbial strains; selection of low-cost renewable raw materials; and more efficient separation processes of the produced PHAs. This review summarizes the main advantages and disadvantages of different strategies proposed to improve the competitiveness of PHAs production; potential applications of PHAs manufactured by these different methods, as well as the main challenges to be overcome to develop economically viable industrial processes, are discussed in detail.

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“…Ensaios preliminares indicaram que a enzima tem boa atividade sobre a forma comercial da pectina, com alto grau de esterificação, e que as variáveis concentração de enzima e agitação têm influência direta sobre a variável resposta concentração de CR (LOCATELLI et al, 2011).…”

Section: Hidrólise Enzimáticaunclassified

“…Mas não foram encontrados na literatura trabalhos que relatem o emprego dos resíduos sólidos ou os produtos de hidrólise da pectina para a produção de PHA's por C. necator. Trabalhos anteriores do grupo demonstraram a capacidade de crescimento celular de C. necator utilizando ácido galacturônico e hidrolisados pécticos como única fonte de carbono (LOCATELLI et al, 2011, LOCATELLI, 2012. Desta forma, o objetivo deste trabalho foi investigar as condições de hidrólise enzimática da pectina que favorecessem a obtenção de CR por meio da aplicação de planejamento fatorial e metodologia de superfície de resposta e utilizar esse substrato na formulação de meio de cultura para crescimento celular e produção de PHA's por C. necator.…”

Section: Introductionunclassified

HIDRÓLISE ENZIMÁTICA DE PECTINA PARA A PRODUÇÃO DE POLIHIDROXIALCANOATOS POR Cupriavidus necator

Locatelli¹,

Finkler²,

Finkler³

2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -Resíduos agroindustriais, provenientes da extração de sucos de frutas cítricas, são ricas fontes de substâncias pécticas. Objetivando o aproveitamento desses resíduos como uma alternativa de baixo custo para a produção de biopolímeros, foi estudado o uso de hidrolisados pécticos para crescimento celular e produção de polihidroxialcanoatos por Cupriavidus necator. Nesse trabalho foi empregada uma pectina cítrica comercial (Vetec ® ) e uma endo-poligalacturonase (Sigma ® ), sendo realizado um estudo das condições de hidrólise que favorecessem a obtenção de compostos redutores (CR) por meio da aplicação de planejamento fatorial e metodologia de superfície de resposta. As variáveis investigadas foram a concentração de enzima e a agitação, mantendo-se a concentração de substrato em 1% (p/v). Os resultados obtidos foram comparados com a performance enzimática realizada a uma maior concentração de substrato (10% p/v) empregando-se diferentes concentrações de enzima. Ambos os resultados indicaram que a concentração enzimática de 10 UI/g de pectina favoreceu a obtenção de CR, permitindo atingir em média 70 g/L de CR a partir de 100 g/L de pectina. O meio de cultura formulado a partir desse hidrolisado foi satisfatório para o crescimento celular de C. necator, sendo obtida uma concentração de células acima de 10 g/L e o acúmulo intracelular de polihidroxialcanoatos.

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Acid Hydrolysis of Pectin for Cell Growth of Cupriavidus necator

Cited by 6 publications

References 21 publications

Facile Synthesis Strategy to Create Mesoporous Magnetic Iron Oxides Using Pectin‑Based Precursors

Facile Synthesis Strategy to Create Mesoporous Magnetic Iron Oxides Using Pectin‑Based Precursors

Polyhydroxyalkanoates Production: A Challenge for the Plastic Industry

HIDRÓLISE ENZIMÁTICA DE PECTINA PARA A PRODUÇÃO DE POLIHIDROXIALCANOATOS POR Cupriavidus necator

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