Acid Degradation of Lignin. IV. Analysis of Lignin Acidolysis Products by Gas Chromatography, Using Trimethylsilyl Derivatives.

Lundquist, Knut; Kirk, T. Kent; Fredlund, Folke; Beronius, Per; Engebretsen, Jan E.; Ehrenberg, L.

doi:10.3891/acta.chem.scand.25-0889

Cited by 63 publications

(27 citation statements)

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“…The ethyl acetate extract was vacuum dried. The ethyl acetate extract was derivatized using trimethyl silyl (BSTFA (N, O-bis (trimethylsilyl) trifluoroacetamide) TMCS) [18]. An aliquot of 1 L of silylated compounds was injected in the injector port of a PE Autosystem XL gas chromatograph interfaced with a Turbomass mass spectrometric mass selective detector (Perkin Elmer, Waltham, MA, USA).…”

Section: Gas Chromatography-mass Spectroscopy (Gc-ms)mentioning

confidence: 99%

Evaluation of the Phytotoxic and Genotoxic Potential of Pulp and Paper Mill Effluent Using Vigna radiata and Allium cepa

Haq

Kumari

Kumar

et al. 2016

Advances in Biology

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Pulp and paper mill effluent induced phytotoxicity and genotoxicity in mung bean (Vigna radiata L.) and root tip cells of onion (Allium cepa L.) were investigated. Physicochemical characteristics such as electrical conductivity (EC), biological oxygen demand (BOD 5 ), chemical oxygen demand (COD), and total phenols of the pulp and paper mill effluent were beyond the permissible limit specified for the discharge of effluent in inland water bodies. Compared to control plants, seedling exposed to 100% effluent concentration showed a reduction in root and shoot length and biomass by 65%, 67%, and 84%, respectively, after 5 days of treatment. A. cepa root tip cells exposed to effluent concentrations ranging from 25 to 100% v/v showed a significant decrease in mitotic index (MI) from 32 to 11% with respect to control root tip cells (69%) indicating effluent induced cytotoxicity. Further, the effluent induced DNA damage as evidenced by the presence of various chromosomal aberrations like stickiness, chromosome loss, anaphase bridge, c-mitosis, tripolar anaphase, vagrant chromosome, and telophase bridge and micronucleated and binucleated cell in A. cepa. Findings of the present study indicate that pulp and paper mill effluents may act as genotoxic and phytotoxic agents in plant model system.

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Section: Gas Chromatography-mass Spectroscopy (Gc-ms)mentioning

confidence: 99%

Evaluation of the Phytotoxic and Genotoxic Potential of Pulp and Paper Mill Effluent Using Vigna radiata and Allium cepa

Haq

Kumari

Kumar

et al. 2016

Advances in Biology

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“…Some wet chemical degradation methods, such as nitrobenzene oxidation, [1] permanganate oxidation, [2] hydrogenolysis, [3] acidolysis, [4] thioacidolysis, [5] ozonation, [6,7] DFRC, [8,9] and TIZ method, [10] have been proposed and employed for understanding lignin structures. Interunitary linkages in a lignin preparation are cleaved by those methods to provide some information on the frequency of the linkages from its low-molecular-weight degradation products.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Reaction Efficiency of Thioacidolysis for Cleavage ofβ-O-4 Interunitary Linkages by Usingβ-O-4 Type Artificial Lignin Polymer

Kanazawa¹,

Kishimoto²,

Koda³

et al. 2009

Journal of Wood Chemistry and Technology

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Dimeric lignin model compounds with non-phenolic and phenolic moieties and a β-O-4 type artificial lignin polymer were subjected to thioacidolysis to evaluate the reaction efficiency of thioacidolysis for cleavage of β-O-4 interunitary linkage. The obtained yields of thioethylated monomeric products from the dimeric lignin model compounds reached nearly 100% under the conventional condition, whereas that from the artificial lignin polymer was as low as 74%. Neither prolonged reaction time nor increased concentration of ethanethiol (the thioacidolysis reagent) enhanced the resulting monomer yields from the polymer (69-79%). Thioacidolysis of the lignin model compounds followed by HPSEC analysis also showed the dimeric model compounds were degraded almost quantitatively, but that the artificial lignin polymer was not. Thioacidolysis followed by desulfurization gave at least one dimeric product resulting from incomplete β-O-4 cleavage at significant yield. These results suggested the conventional thioacidolysis could not achieve quantitative cleavage of β-O-4 linkages in lignin macromolecules.

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“…In these methods, the most commonly present (70% of lignin) arylglycerol-β-arylether bond in lignin is broken, resulting in end products such as cinnamic acid, benzaldehyde, phenones and quinoids [56]. The thioacidolysis method has an advantage over acidolysis in detecting the 5-5′ and 5-O-4′ bonded dimers.…”

Section: Degradative Analytical Techniques (Oxidation Reduction Hydmentioning

confidence: 99%

“…However, both these methods have limitations in detecting compounds that contain only β-arylether bonds. Also, the use of highly acidic conditions makes it difficult to identify the end products obtained from these two methods [56].…”

Section: Degradative Analytical Techniques (Oxidation Reduction Hydmentioning

confidence: 99%

Engineering Plant Biomass Lignin Content and Composition for Biofuels and Bioproducts

et al. 2015

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Abstract:Lignin is an aromatic biopolymer involved in providing structural support to plant cell walls. Compared to the other cell wall polymers, i.e., cellulose and hemicelluloses, lignin has been considered a hindrance in cellulosic bioethanol production due to the complexity involved in its separation from other polymers of various biomass feedstocks. Nevertheless, lignin is a potential source of valuable aromatic chemical compounds and upgradable building blocks. Though the biosynthetic pathway of lignin has been elucidated in great detail, the random nature of the polymerization (free radical coupling) process poses challenges for its depolymerization into valuable bioproducts. The absence of specific methodologies for lignin degradation represents an important opportunity for research and development. This review highlights research development in lignin biosynthesis, lignin genetic engineering and different biological and chemical means of depolymerization used to convert lignin into biofuels and bioproducts. OPEN ACCESSEnergies 2015, 8 7655

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Acid Degradation of Lignin. IV. Analysis of Lignin Acidolysis Products by Gas Chromatography, Using Trimethylsilyl Derivatives.

Cited by 63 publications

References 0 publications

Evaluation of the Phytotoxic and Genotoxic Potential of Pulp and Paper Mill Effluent Using Vigna radiata and Allium cepa

Evaluation of the Phytotoxic and Genotoxic Potential of Pulp and Paper Mill Effluent Using Vigna radiata and Allium cepa

Evaluation of Reaction Efficiency of Thioacidolysis for Cleavage ofβ-O-4 Interunitary Linkages by Usingβ-O-4 Type Artificial Lignin Polymer

Engineering Plant Biomass Lignin Content and Composition for Biofuels and Bioproducts

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