Acid‐citrate‐dextrose‐phosphoenolpyruvate medium as a rejuvenant for blood storage

Hamasaki, N; Hirota-Chigita, C

doi:10.1046/j.1537-2995.1983.23183147295.x

Cited by 25 publications

(6 citation statements)

References 13 publications

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“…However, even though abnormal RBC shapes were seen, one study reported that the morphological changes after thawing using HES recovered rapidly in fresh autologous plasma [21]. Further research is required to determine the effects that ATP and 2,3-DPG have on RBC membranes using rejuventation [1,3,14,24] and to determine the biochemical changes to RBC membranes.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of the Effects of Glycerol and Hydroxyethyl Starch in Canine Red Blood Cell Cryopreservation

et al. 2004

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ABSTRACT. Hydroxyethyl starch (HES) is a nonpenetrating extracellular cryoprotectant. In contrast to glycerol, it does not require laborintensive removal from thawed red blood cells (RBCs) prior to transfusion. In this study, we compared glycerol and HES, and assessed HES as a substitute for glycerol in cryopreserved canine RBCs. The RBCs were preserved for 2 months in liquid nitrogen using a 20% (w/v) glycerol solution, and variable concentrations of HES solution. We evaluated the two cryoprotectants by the percentage of postthaw hemolysis from the total free hemoglobin, saline stability, osmotic fragility, and by observing the erythrocyte morphology using a scanning electron microscope after thawing. The optimal concentration of HES was 12.5% (w/v) for the cryopreservation of canine RBCs. The thaw hemolysis, saline stability, and osmotic fragility index were 25.6 ± 4.7%, 87.8 ± 6.9%, and 0.445 ± 0.024% NaCl respectively. These parameters resemble the results of RBCs frozen in a 20% (w/v) glycerol solution, which are 24.7 ± 5.2%, 99.2 ± 0.1%, and 0.485 ± 0.023% NaCl respectively. From a morphological point of view, 12.5% (w/v) HES showed the best cryoprotection of RBCs compared to the other concentrations of HES. These results suggest that HES could be a possible substitute for glycerol for the cryopreservation of canine RBCs.

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Section: Discussionmentioning

confidence: 99%

A Comparative Study of the Effects of Glycerol and Hydroxyethyl Starch in Canine Red Blood Cell Cryopreservation

et al. 2004

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“…Ad ditions of chemicals such as bicarbonate [8], dihydroxyacetone [9], and phosphoenolpyruvate [10] have been tried but abandoned, often for practical or economical reasons. In recent studies [11] we have shown that a combination of half-strength citrate CPD [12] with a new additive solution for red cell suspension (RAS), inspired by the work of Meryman et al [13], results in good maintenance of BPG without loss of adenine nucleotides.…”

Section: Anticoagulants and Additive Solutionsmentioning

confidence: 99%

Half-Strength Citrate CPD Combined with a New Additive Solution for Improved Storage of Red Blood Cells Suitable for Clinical Use

Högman¹,

Gong²,

Högman³

et al. 1993

Vox Sanguinis

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Currently used systems for red blood cell (RBC) collection and storage for transfusion have the disadvantage that the RBC 2,3-bisphosphoglycerate (BPG) concentration is depleted within two weeks of storage, resulting in a left-shift of the oxygen dissociation curve and a temporarily impaired capacity to deliver oxygen. We have studied the effects on red cell metabolism, morphology and in vivo recovery of 49-day storage of RBC, with collection in half-strength citrate CPD (0.5CPD) and storage in an additive solution containing citrate, adenine, mannitol, phosphate and glucose (RAS2). Traditional CPD-SAGM was used for comparison. Component preparation was performed after an initial holding period of the whole blood at ambient temperature for 8h. The BPG concentration in 0.5CPD-RAS2 RBC was 0.633±0.120 mol (mol Hb)^-1 as compared to 0.454±0.138 mol (mol Hb)^-1 in CPD-SAGM RBC which implied a decrease to 67 and 48% of normal concentration, respectively. The mean RBC BPG concentration was maintained at the initial level for 28 days in the new system but decreased to very low levels within 14 days in the controls. The total adenine nucleotides were well maintained in both systems, adenosine triphosphate slightly better in the new system. Hemolysis after 49 days was 0.35±0.21% in the new system and 0.72±0.25% in the controls (p<0.001). The morphology was better maintained in the new system (p<0.001). The 24-hour posttransfusion survival of 49-day stored RBC was 78.9±7.1%. The membrane leakage of sodium and potassium was not significantly different in the two systems. The concentration of inorganic phosphate (Pi) in the extracellular fluid of 0.5 CPD-RAS2 RBC was initially 7.1±0.8 mmol l^-1. It decreased to approximately 5.5 mmol l^-1 during the first 2 weeks of storage and then slowly increased to 9.4±0.9 mmol l^-1 on day 49. Pi in the CPD-SAGM controls was low initially but rose to 6.2±0.4 mmol l^-1 at the end of storage. The new system offers clearcut advantages as compared to traditional systems and does not seem to raise any serious clinical objections against its use even in massive transfusions.

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“…A certain 'rejuvenlating' effect appears to occur, however, 2,3-DPG level was not maintained even by this method (data not shown). A more effective reagent such a s phosphoenolpyruvate (Hamasaki et al, 1981;Hamasaki & Hirota-Chigita, 1983) or PIGPA solution (DeVenuto rt a/., 1974) may be required a t the final stage of storage to achieve this.…”

Section: Discussionmentioning

confidence: 99%

The preservative‐exchange method using a sextuple‐bag system for a 10‐week storage period of red blood cells

Ohkuma

Miyahara

Nishizaki

1991

Transfusion Medicine

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Prolonged storage of red blood cells in a liquid state was achieved by replacing the preservative using a sextuple-bag system. The bag system consists of one primary bag containing citrate-phosphate-dextrose (CPD) solution, three satellite bags containing saline-adenine-glucose-phosphate-maltose (SAGP-maltose) solution, and two empty satellite bags to remove plasma and buffy coat. Preservative can be exchanged three times in this closed system. The system is able to supply nutrients, such as glucose, and to remove harmful metabolites, such as lactic acid, by exchanging the preservative during storage. As a result, red cells stored by this method showed much higher levels of total adenylate and morphological score after the second preservative exchange, when compared with red cells stored by the conventional method (P < 0.01). Judging from these two in-vitro parameters, red cells may tolerate storage for at least 10 weeks in a liquid state. This method might be useful for 'predeposit autologous transfusion', as it is more convenient and more cost effective than the freeze-preservation method.

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Acid‐citrate‐dextrose‐phosphoenolpyruvate medium as a rejuvenant for blood storage

Cited by 25 publications

References 13 publications

A Comparative Study of the Effects of Glycerol and Hydroxyethyl Starch in Canine Red Blood Cell Cryopreservation

A Comparative Study of the Effects of Glycerol and Hydroxyethyl Starch in Canine Red Blood Cell Cryopreservation

Half-Strength Citrate CPD Combined with a New Additive Solution for Improved Storage of Red Blood Cells Suitable for Clinical Use

The preservative‐exchange method using a sextuple‐bag system for a 10‐week storage period of red blood cells

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