Acid-base dissociation mechanisms and energetics at the silica–water interface: An activationless process

Lowe, Benjamin; Skylaris, Chris‐Kriton; Green, Nicolas G.

doi:10.1016/j.jcis.2015.01.094

Cited by 102 publications

(70 citation statements)

References 78 publications

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“…174,175 The theoretical detection time for an individual proton is non-trivial to calculate due to the complexity introduced by acid-base equilibria and Grotthuss transport, which cannot be described by simple Brownian motion. 176 In summary, for BioFET design the limitations imposed by the mass transport, binding kinetics and geometry choices should be considered when designing for optimal response. 52,147,169,177 Non-specific response.…”

Section: Critical Review Analystmentioning

confidence: 99%

Field-effect sensors – from pH sensing to biosensing: sensitivity enhancement using streptavidin–biotin as a model system

Lowe

Sun

Zeimpekis

et al. 2017

Analyst

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117

104

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a Field-Effect Transistor sensors (FET-sensors) have been receiving increasing attention for biomolecular sensing over the last two decades due to their potential for ultra-high sensitivity sensing, label-free operation, cost reduction and miniaturisation. Whilst the commercial application of FET-sensors in pH sensing has been realised, their commercial application in biomolecular sensing (termed BioFETs) is hindered by poor understanding of how to optimise device design for highly reproducible operation and high sensitivity. In part, these problems stem from the highly interdisciplinary nature of the problems encountered in this field, in which knowledge of biomolecular-binding kinetics, surface chemistry, electrical double layer physics and electrical engineering is required. In this work, a quantitative analysis and critical review has been performed comparing literature FET-sensor data for pH-sensing with data for sensing of biomolecular streptavidin binding to surface-bound biotin systems. The aim is to provide the first systematic, quantitative comparison of BioFET results for a single biomolecular analyte, specifically streptavidin, which is the most commonly used model protein in biosensing experiments, and often used as an initial proofof-concept for new biosensor designs. This novel quantitative and comparative analysis of the surface potential behaviour of a range of devices demonstrated a strong contrast between the trends observed in pH-sensing and those in biomolecule-sensing. Potential explanations are discussed in detail and surfacechemistry optimisation is shown to be a vital component in sensitivity-enhancement. Factors which can influence the response, yet which have not always been fully appreciated, are explored and practical suggestions are provided on how to improve experimental design.

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Section: Critical Review Analystmentioning

confidence: 99%

Field-effect sensors – from pH sensing to biosensing: sensitivity enhancement using streptavidin–biotin as a model system

Lowe

Sun

Zeimpekis

et al. 2017

Analyst

Self Cite

117

104

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“…The Nandoni Dam is also surrounded by activities that involve discharges from a sewer network, irrigation canals, drainage network and pumped groundwater which find their way to the lake via several tributaries. These activities result to the pH fluctuations depending on their hydronium and hydroxide ion dissociation potentials in water [28,29].…”

Section: Physico-chemical Analysismentioning

confidence: 99%

Spectroscopic Determination of Water Salinity in Brackish Surface Water in Nandoni Dam, at Vhembe District, Limpopo Province, South Africa

Nthunya

Maifadi

Mamba

et al. 2018

Water

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Abstract:The problem of limited water supply in the Vhembe District (Limpopo Province, South Africa) is exacerbated by a preponderance of dissolved salts, which cause disagreeable taste and odour in the water as reported by the communities using this water for drinking. The water treatment plant that supplies the treated water to the communities in the District sources this raw water from the Nandoni Dam at the Luvuvhu river catchment. There are no scientific studies that have been reported in the literature that focused on determining the levels of water salinity from various water sources in the municipalities of the District. Water samples from various sites across the Nandoni Dam, a primary source of domestic water supply in the region, were collected through each season over a period of twelve months in order to ascertain the concentrations of dissolved salts in the dam. Onsite analyses of the water samples were conducted using the YSI ProDSS multimeter, while the laboratory water analyses were conducted using the spectroquant and atomic absorption spectrometers. Although salinity tests seem to indicate that the water sampled across most of the Nandoni Dam is brackish during all seasons of the year with the highest being 750 mg/L, water samples from the dam mid-outlet and the treatment plant are slightly below the World Health Organization (WHO) brackish water bracket of 500 mg/L with unfavourable taste for drinking. Results from this study indicate that the water sourced from the Nandoni Dam is not suitable for human consumption and therefore requires integrated water resource management, as well as robust and cost-effective water desalination treatment.

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“…These results can be explained by consideration of interactions between protonated peptides and residual silanol groups of the stationary phase. At the mobile phase pH (between 3 and 3.5), residual silanols of the stationary phase are partially ionized, and the ionized silanols exist as anions . A triply charged peptide has a stronger interaction with the negatively charged silanols than a doubly charged peptide, based on the number of positive charges involved alone.…”

Section: Resultsmentioning

confidence: 99%

“…At the mobile phase pH (between 3 and 3.5), residual silanols of the stationary phase are partially ionized, and the ionized silanols exist as anions. 32 A triply charged peptide has a stronger interaction with the negatively charged silanols than a doubly charged peptide, based on the number of positive charges involved alone. If the synergistic effect is taken into consideration, 18,33 the interaction between a triply charged peptide and the silanols is significantly stronger than that between a doubly charged peptide and the silanols.…”

Section: Assessing In Vitro Degradation Of Peptides In Equine Plasmamentioning

confidence: 99%

A comprehensive approach to detecting multitudinous bioactive peptides in equine plasma and urine using hydrophilic interaction liquid chromatography coupled to high resolution mass spectrometry

Guan

You

et al. 2019

Drug Testing and Analysis

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Bioactive peptides possess pharmacological effects and can be illicitly used in sports. To deter such misuse, an untargeted method using high resolution mass spectrometry (HRMS) has been developed for comprehensive detection of multitudinous exogenous peptides in equine plasma and urine. Forty‐four peptides were extracted using mixed‐mode solid‐phase extraction (SPE) from plasma and urine, separated with a hydrophilic interaction liquid chromatography (HILIC) column, and detected on an HRMS instrument. Ammonium formate as a mobile phase additive had effects on HILIC retention and charge state distribution of the peptides. The acetonitrile percentage in the reconstitution solution affected the solubility of peptide neat standards and peptides in plasma and urine extracts differently. The stability of the peptides in plasma at ambient temperature was assessed. The limit of detection (LOD) was 10–50 pg/mL for most of the peptides in plasma, and ≤ 500 pg/mL for the remaining. LOD was 100–400 pg/mL for the majority of the analytes in urine, and ≤ 4000 pg/mL for the others. The method was used successfully to analyze incurred plasma and urine samples from research horses administered dermorphin. Even in the absence of reference standards, dermorphin metabolites (aFGYPS‐NH2, YaFG, and YaF) were identified. These results demonstrate that data generated with this method can be retrospectively reviewed for peptides that are unknown at the time of sample analysis without requiring re‐analysis of the sample. This method provides a powerful novel tool for detection of numerous bioactive peptides and their metabolites in equine plasma and urine for doping control.

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Acid-base dissociation mechanisms and energetics at the silica–water interface: An activationless process

Cited by 102 publications

References 78 publications

Field-effect sensors – from pH sensing to biosensing: sensitivity enhancement using streptavidin–biotin as a model system

Field-effect sensors – from pH sensing to biosensing: sensitivity enhancement using streptavidin–biotin as a model system

Spectroscopic Determination of Water Salinity in Brackish Surface Water in Nandoni Dam, at Vhembe District, Limpopo Province, South Africa

A comprehensive approach to detecting multitudinous bioactive peptides in equine plasma and urine using hydrophilic interaction liquid chromatography coupled to high resolution mass spectrometry

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