Acid and alkali etching of grit blasted zirconia: Impact on adhesion and osteogenic differentiation of MG63 cells in vitro

Abstract

There is a need for evaluating zirconia surface modifications and their potential impact on the biological response of osteogenic cells. Grit blasted zirconia discs were either left untreated or underwent acid or alkaline etching. Adhesion and osteogenic differentiation of MG63 cells was determined after one week of culture. The macro-scaled roughness of the grit blasted zirconia discs, independent of the surface treatment, was within a narrow range and only slightly smoother than titanium discs. However, the alkaline-and acid-etching led to an increase of the micro-roughness of the surface. The surface modifications had no effect on cell spreading and did not cause significant change in the expression of differentiation markers. Thus, in this respective setting, morphologic changes observed upon treatment of grit blasted zirconia discs with acid or alkaline do not translate into changes in MG63 cell adhesion or differentiation and are comparable to findings with anodized titanium discs.

Acid and Alkaline Etching of Sandblasted Zirconia Implants: A Histomorphometric Study in Miniature Pigs

“…38 Although the surface roughness parameters in a recently published study were rather similar among the three examined surface modifications, the scanning electron microscope pictures clearly showed a different surface topography. 39 In the present study, a similar observation was made. To explain if these morphological changes caused the increased MNGCs requires further studies, including in vitro assays.…”

“…In vitro studies suggest that surface topography has an impact on macrophage activation and thus the secretion of proinflammatory cytokines and chemokines . Although the surface roughness parameters in a recently published study were rather similar among the three examined surface modifications, the scanning electron microscope pictures clearly showed a different surface topography . In the present study, a similar observation was made.…”

Regulating the osteogenic function of rhBMP 2 by different titanium surface properties

“…It is quite suitable for the application under loading‐bearing conditions. The acid alkali treatment is another commonly used surface modification method . Similar to the anodic oxidation, this method can improve bone regeneration by attracting Ca 2+ ions and ions orderly.…”

Surface roughened zirconia: towards hydrothermal stability

“…Gahlert et al evidenced that hydrofluoric acid (HF) etching of zirconia implants enhances bone apposition resulting in high removal torque values (Gahlert et al, 2010). Other chemicals, such as hypophosphorous acid or an equimolar mixture of potassium hydroxide and sodium hydroxide, have been reported to successfully etch Y-TZP (Casucci et al, 2010;Gruber et al, 2012).…”