Acholeplasma morum, a New Non-Sterol-Requiring Species

Rose, David L.; Tully, Joseph G.; Giudice, Richard A. Del

doi:10.1099/00207713-30-4-647

Cited by 17 publications

(5 citation statements)

References 6 publications

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“…Isolates were routinely grown in MC broth or in the serum fraction medium described previously (17). We developed a serum-free medium that contained mycoplasma broth base (BBL Microbiology Systems, Cockeysville, Md.)…”

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Acholeplasma florum, a New Species Isolated from Plants

McCoy

Basham

Tully

et al. 1984

International Journal of Systematic Bacteriology

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Three acholeplasmas isolated from floral surfaces of healthy plants in Florida were found to be similar in their biochemical and serological properties. These organisms did not require serum or cholesterol for growth, although addition of some supplementary fatty acids (as represented by Tween 80) was necessary for growth to occur in serum-free medium. The three strains possessed biochemical properties typical of the Acholeplasmataceae and were distinguished from the nine previously recognized Acholeplasma species by serological and deoxyribopucleic acid-deoxyribonucleic acid hybridization techniques. The genome molecular weight of the three Acholeplasma strains was lo9, and the guanine-plus-cytosine content of the deoxyribonucleic acid was 27 to 28 mol%. On the basis of these results and other morphological, biological, and serological properties, we propose that these organisms represent a new species, Acholeplasmaflorurn. Strain L1 (= ATCC 33453) is the type strain.Plant surfaces, particularly flowers, have recently been proven to be fertile sites for isolation of members of the Mycoplasrnatales (5,(11)(12)(13)26 19), species that have been previously identified as having animal origins (22). However, although three isolates from citrus and ornamental flowers in Florida were related to each ather, they were not related to any previously described Acholeplasrna species. In this paper we define the basic characteristics of these flower surface acholeplasmas and describe their unique biological properties. We propose that these organisms be given taxonomic status as a new species of Acholeplasrna. MATERIALS AND METHODSAchdeplusmu strains. Details of the primary isolation technique for flower surface-inhabiting strains have been described previously (13). Strain PP2 was obtained from blossoms of Calliandra haematocephalus, whereas strains LIT (T = type strain) and GF1 were obtained from flowers of lemon (Citrus limon) and grapefruit (Citrus paradisi), respectively. Primary isolation was in MC (14) or SP-4 (24) broth medium without antibiotics. Each Acholeplasma strain was purified by a 3 x filtration-cloning technique (21) 4782.Media and cultivation procedures. Isolates were routinely grown in MC broth or in the serum fraction medium described previously (17). We developed a serum-free medium that contained mycoplasma broth base (BBL Microbiology Systems, Cockeysville, Md.) supplemeuted with 10% fresh 25% yeast extract (Microbiological Associates, Bethesda, Md.), 0.5% glucose, 500 U of penicillin G per ml, 0.002% phenol red, 0.5% bovine serum albumin, 0.04% Tween 80, and 10 pg of palmitic acid per ml. Solid medium was prepared by adding 0.8% Noble agar (Difco Laboratories, Detroit, Mich.) or 1.0% agarose to the broth base before autoclaving. Cultures were grown aerobically at 25 to 30°C.Reversion studies. Primary isolation of strains LIT, PP2, and GF1 was in antibiotic-free media, and more than 30 passages were made in the absence of penicillin. In addition, transfer of these strains to penicillin G-containing s...

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Acholeplasma florum, a New Species Isolated from Plants

McCoy

Basham

Tully

et al. 1984

International Journal of Systematic Bacteriology

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“…Using labeled RNA-DNA hybrid-ization techniques, he reported 32 to 34% homology between A. granularum and A. laidlawii. Recently, we have used nick-translation techniques to show a lack of DNA homology between the established species of Acholeplasma and new strains of acholeplasmas isolated from tissue cultures (2,24) and from plants (26). We report here the relationships among the eight currently recognized Acholeplasma species as determined by DNA-DNA hybridization and serological procedures.…”

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Nucleic acid relationships among Acholeplasma species

Aulakh¹,

Stephens²,

Rose³

et al. 1983

J Bacteriol

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3H-labeled Acholeplasma DNA probes were generated in vitro by the nicktranslation method and used to determine the nucleotide sequence homology among the type strains of the eight currently recognized species of Acholeplasma. Very little nucleotide sequence homology (l18%) was found among the eight species, with heteroduplexes showing at least 12% or more mismatching as determined by thermal elution midpoints. The small amount of nucleotide sequence homology among the eight species indicates that these species are quite distinct and are not closely related to each other genomically.

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“…The description is the same as that for Acholeplasma morum given by Rose et al . [45]. The type strain is 72-043 T (=ATCC 33211 T =NCTC 10188 T ).…”

Section: Description Of Paracholeplasma Morum Comb Novmentioning

confidence: 99%

Mariniplasma anaerobium gen. nov., sp. nov., a novel anaerobic marine mollicute, and proposal of three novel genera to reclassify members of Acholeplasma clusters II–IV

Watanabe

Kojima

Okano

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel strictly anaerobic chemoorganotrophic bacterium, designated Mahy22T, was isolated from sulfidic bottom water of a shallow brackish meromictic lake in Japan. Cells of the strain were Gram-stain-negative, non-motile and coccoid in shape with diameters of about 600–800 nm. The temperature range for growth was 15–37 °C, with optimum growth at 30–32 °C. The pH range for growth was pH 6.2–8.9, with optimum growth at pH 7.2–7.4. The strain grew with NaCl concentrations of 5% or below (optimum, 2–3%). Growth of the strain was enhanced by the addition of thiosulfate. The major cellular fatty acids were C16:0 and anteiso-C15:0. Respiratory quinones were not detected. The complete genome sequence of strain Mahy22T possessed a 1 885 846 bp circular chromosome and a 12 782 bp circular genetic element. The G+C content of the genome sequence was 30.1 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family Acholeplasmataceae , class Mollicutes . The closest relative of strain Mahy22T with a validly published name was Acholeplasma palmae J233T with a 16S rRNA gene sequence similarity of 90.5%. Based on the results of polyphasic analysis, the name Mariniplasma anaerobium gen. nov., sp. nov. is proposed to accommodate strain Mahy22T, along with reclassification of some Acholeplasma species into Alteracholeplasma gen. nov., Haploplasma gen. nov. and Paracholeplasma gen. nov.

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Acholeplasma morum, a New Non-Sterol-Requiring Species

Cited by 17 publications

References 6 publications

Acholeplasma florum, a New Species Isolated from Plants

Acholeplasma florum, a New Species Isolated from Plants

Nucleic acid relationships among Acholeplasma species

Mariniplasma anaerobium gen. nov., sp. nov., a novel anaerobic marine mollicute, and proposal of three novel genera to reclassify members of Acholeplasma clusters II–IV

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