Acheron/Larp6 Is a Survival Protein That Protects Skeletal Muscle From Programmed Cell Death During Development

Sheel, Ankur; Shao, Rong; Brown, Christine; Johnson, Joanne E.; Hamilton, Alexandra; Sun, Danhui; Oppenheimer, Julia; Smith, Wendy A.; Visconti, Pablo E.; Markstein, Michele; Bigelow, Carol; Schwartz, Lawrence M.

doi:10.3389/fcell.2020.00622

Cited by 9 publications

(6 citation statements)

References 68 publications

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“…LARP6 , one of the co-expressed genes associated with the lncRNA G8399 encodes a protein that prevents cell death until it is triggered by dephosphorylation and degraded ( Sheel et al, 2020 ). We also found that EOMES, one of the target genes for the lncRNA G8399, is involved in the trophoblast differentiation as well as gastrulation, regulating both the mesodermal delamination, endodermal specification, which are essential for developing the tissues extraembryonic to the uterus during the mammalian embryogenesis ( Kidder and Palmer, 2010 ; Probst and Arnold, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

Gao

Zhang

et al. 2021

Front. Genet.

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Granulosa cells (GCs) are decisive players in follicular development. In this study, the follicle tissues and GCs were isolated from the goose during the peak-laying period to perform hematoxylin-eosin staining and RNA-seq, respectively. Moreover, the dynamic mRNA and lncRNA expression profiles and mRNA-lncRNA network analysis were integrated to identify the important genes and lncRNAs. The morphological analysis showed that the size of the GCs did not significantly change, but the thickness of the granulosa layer cells differed significantly across the developmental stages. Subsequently, 14,286 mRNAs, 3,956 lncRNAs, and 1,329 TUCPs (transcripts with unknown coding potential) were detected in the GCs. We identified 37 common DEGs in the pre-hierarchical and hierarchical follicle stages, respectively, which might be critical for follicle development. Moreover, 3,089 significant time-course DEGs (Differentially expressed genes) and 13 core genes in 4 clusters were screened during goose GCs development. Finally, the network lncRNA G8399 with CADH5 and KLF2, and lncRNA G8399 with LARP6 and EOMES were found to be important for follicular development in GCs. Thus, the results would provide a rich resource for elucidating the reproductive biology of geese and accelerate the improvement of the egg-laying performance of geese.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

Gao

Zhang

et al. 2021

Front. Genet.

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“…Likewise, the interconnection between apoptosis and mitophagy shows interactions between both protagonists and even a dual role for some proteins [1]. In mammals, BNIP3 is associated with a pro-apoptotic function [24], whereas, in Drosophila, few studies have focused on the role of BNIP3 in mitophagy [25][26][27], and, to our knowledge, only one explored the role of BNIP3 in apoptosis [28]. Concerning PINK1, Han et al observed increased levels of apoptosis induced by a mutation altering the PINK1 kinase activity in Drosophila [29].…”

Section: Introductionmentioning

confidence: 99%

PINK1 and BNIP3 mitophagy inducers have an antagonistic effect on Rbf1-induced apoptosis inDrosophila

Fages,

Ruby,

Brusson

et al. 2023

Preprint

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The structure and function of the mitochondrial network are finely regulated. Among the proteins involved in these regulations, mitochondrial dynamics actors have been reported to regulate the apoptotic process. We show here in the Drosophila model that the mitophagy inducers, PINK1 (PTEN-induced putative kinase 1) and BNIP3 (Bcl-2 Interacting Protein 3), modulate mitochondrial apoptosis differently. If close links between the fission-inducing protein DRP1 and Bcl-2 family proteins, regulators of apoptosis, are demonstrated, the connection between mitophagy and apoptosis is still poorly understood. In Drosophila, we have shown that Rbf1, a homolog of the oncosuppressive protein pRb, induces cell death in proliferating larval tissues through a mechanism involving the interaction of Drp1 with Debcl, a pro-apoptotic protein of the Bcl-2 family. This interaction is necessary to induce mitochondrial fission, ROS production, and apoptosis. To better understand the interactions between the proteins involved in mitochondrial homeostasis and the apoptotic process, we focused on the role of two known players in mitophagy, the proteins PINK1 and BNIP3, during mitochondrial apoptosis induced by Rbf1 and Debcl in a proliferating Drosophila larval tissue. We show that Rbf1- or Debcl-induced apoptosis is accompanied by mitophagy. Interestingly, PINK1 and BNIP3 have distinct effects in regulating cell death. PINK1 promotes rbf1- or debcl-induced apoptosis, whereas BNIP3 protects against Rbf1-induced apoptosis but reduces Debcl-induced tissue loss without inhibiting Debcl-induced cell death. Furthermore, our results indicate that BNIP3 is required to induce basal mitophagy while PINK1 is responsible for mitophagy induced by rbf1 overexpression. These results highlight the critical role of mitophagy regulators in controlling homeostasis and cell fate.

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“…The ISMs are a classical model system for skeletal muscle atrophy and death ( Schwartz and Truman 1982 ; Schwartz 2018 ; Sheel et al, 2020 ). These muscles are composed of giant syncytial cells, each of which is about 5 mm long and up to 1 mm in diameter.…”

Section: Introductionmentioning

confidence: 99%

Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death

et al. 2021

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PIWI-interacting RNAs (piRNAs) are small single-stranded RNAs that can repress transposon expression via epigenetic silencing and transcript degradation. They have been identified predominantly in the ovary and testis, where they serve essential roles in transposon silencing in order to protect the integrity of the genome in the germline. The potential expression of piRNAs in somatic cells has been controversial. In the present study we demonstrate the expression of piRNAs derived from both genic and transposon RNAs in the intersegmental muscles (ISMs) from the tobacco hawkmoth Manduca sexta. These piRNAs are abundantly expressed, ∼27 nt long, map antisense to transposons, are oxidation resistant, exhibit a 5’ uridine bias, and amplify via the canonical ping-pong pathway. An RNA-seq analysis demonstrated that 19 piRNA pathway genes are expressed in the ISMs and are developmentally regulated. The abundance of piRNAs does not change when the muscles initiate developmentally-regulated atrophy, but are repressed coincident with the commitment of the muscles undergo programmed cell death at the end of metamorphosis. This change in piRNA expression is correlated with the repression of several retrotransposons and the induction of specific DNA transposons. The developmentally-regulated changes in the expression of piRNAs, piRNA pathway genes, and transposons are all regulated by 20-hydroxyecdysone, the steroid hormone that controls the timing of ISM death. Taken together, these data provide compelling evidence for the existence of piRNA in somatic tissues and suggest that they may play roles in developmental processes such as programmed cell death.

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Acheron/Larp6 Is a Survival Protein That Protects Skeletal Muscle From Programmed Cell Death During Development

Cited by 9 publications

References 68 publications

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

PINK1 and BNIP3 mitophagy inducers have an antagonistic effect on Rbf1-induced apoptosis inDrosophila

Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death

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